PMID- 32887361
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231112
IS  - 2075-4418 (Print)
IS  - 2075-4418 (Electronic)
IS  - 2075-4418 (Linking)
VI  - 10
IP  - 9
DP  - 2020 Sep 2
TI  - Identification the Cross-Reactive or Species-Specific Allergens of Tyrophagus 
      putrescentiae and Development Molecular Diagnostic Kits for Allergic Diseases.
LID - 10.3390/diagnostics10090665 [doi]
LID - 665
AB  - Mite allergens are considerable factors in the genesis of allergic diseases. The 
      storage mite Tyrophagus putrescentiae (Tp) appears in contaminated foods and 
      household surroundings. The current diagnostic tools for Tp allergy are mostly 
      based on crude extracts and still contain shortcomings. This study aimed to 
      investigate the immunoglobulin E (IgE)- responsiveness profiles of Tp-allergic 
      patients and develop a molecular diagnostic method using recombinant allergens. 
      Allergenic components were characterized as cross-reacting or species-specific 
      allergens, in which the effective combinations of recombinant allergens were 
      developed and analyzed in terms of the prediction accuracy for clinical 
      diagnosis. Seven recombinant allergens were cloned and generated to detect the 
      IgE responsiveness of the Tp allergy. A survey on the prevalence of mite allergy 
      showed there were higher sensitizations with IgE responsiveness to house dust 
      mites (HDM) (78.9-80.9%) than to storage mites Tp (35.6%). Prevalence of 
      sensitization to Tp was higher in elderly subjects. The principal IgE-binding 
      components of Tp were Tyr p 1, Tyr p 2 and Tyr p 3. Prediction accuracy for Tp 
      allergy by IgE-responsiveness combination D (Tyr p 1, Tyr p 2 & Tyr p 3) was with 
      high precision (100%). Avoiding the cross-reactivity of Dermatophagoides 
      pteronyssinus, the prediction accuracy of IgE-responsiveness combination H+ (Tyr 
      p 1, Tyr p 2, Tyr p 3, Tyr p 7, Tyr p 8, Tyr p 10 & Tyr p 20) was suitable for 
      Tp-specific diagnosis. Panels of Tp allergens were generated and developed a 
      diagnostic kit able beneficial to identify IgE-mediated Tp hypersensitivity.
FAU - Yu, Ching-Hsiang
AU  - Yu CH
AD  - Department of Medicine, MacKay Medical College, New Taipei City 252, Taiwan.
FAU - Tsai, Jaw-Ji
AU  - Tsai JJ
AD  - Division of Allergy, Immunology & Rheumatology, Department of Internal Medicine, 
      Asia University Hospital, Taichung 413, Taiwan.
FAU - Lin, Yi-Hsueh
AU  - Lin YH
AD  - Institute of Clinical Medicine, National Yang Ming University, Taipei 112, 
      Taiwan.
FAU - Yu, Sheng-Jie
AU  - Yu SJ
AD  - Department of Medical Education and Research, Kaohsiung Veterans General 
      Hospital, Kaohsiung 813, Taiwan.
FAU - Liao, En-Chih
AU  - Liao EC
AUID- ORCID: 0000-0002-8403-3656
AD  - Department of Medicine, MacKay Medical College, New Taipei City 252, Taiwan.
AD  - Department of Medicine, Mackay Medical College No. 46, Sec. 3, Zhongzheng Rd., 
      Sanzhi Dist., New Taipei City 252, Taiwan.
LA  - eng
GR  - 1081B30/MacKay Medical College/
GR  - MMH-MM-10704/MacKay Memorial Hospita/
GR  - MOST 106-2320-B-715-005-MY3/Ministry of Science and Technology, Taiwan, Republic 
      of China/
GR  - MOST 109-2320-B-715-004/Ministry of Science and Technology, Taiwan/
PT  - Journal Article
DEP - 20200902
PL  - Switzerland
TA  - Diagnostics (Basel)
JT  - Diagnostics (Basel, Switzerland)
JID - 101658402
PMC - PMC7555383
OTO - NOTNLM
OT  - Dermatophagoides pteronyssinus
OT  - Tyrophagus putrescentiae
OT  - allergic diseases
OT  - diagnosis technology
OT  - mite allergens
OT  - storage mite
COIS- The authors declared that they have no conflicts of interest.
EDAT- 2020/09/06 06:00
MHDA- 2020/09/06 06:01
PMCR- 2020/09/02
CRDT- 2020/09/05 01:03
PHST- 2020/06/07 00:00 [received]
PHST- 2020/08/26 00:00 [revised]
PHST- 2020/08/28 00:00 [accepted]
PHST- 2020/09/05 01:03 [entrez]
PHST- 2020/09/06 06:00 [pubmed]
PHST- 2020/09/06 06:01 [medline]
PHST- 2020/09/02 00:00 [pmc-release]
AID - diagnostics10090665 [pii]
AID - diagnostics-10-00665 [pii]
AID - 10.3390/diagnostics10090665 [doi]
PST - epublish
SO  - Diagnostics (Basel). 2020 Sep 2;10(9):665. doi: 10.3390/diagnostics10090665.