PMID- 32891815 OWN - NLM STAT- MEDLINE DCOM- 20210226 LR - 20240109 IS - 1872-7573 (Electronic) IS - 0378-8741 (Linking) VI - 265 DP - 2021 Jan 30 TI - Sargassum horneri (Turner) C. Agardh containing polyphenols attenuates particulate matter-induced inflammatory response by blocking TLR-mediated MYD88-dependent MAPK signaling pathway in MLE-12 cells. PG - 113340 LID - S0378-8741(20)33222-0 [pii] LID - 10.1016/j.jep.2020.113340 [doi] AB - ETHNOPHARMACOLOGICAL RELEVANCE: Sargassum horneri (Turner) C. Agardh (S. horneri), an edible brown marine algae, is known to have immunomodulatory effects and has been used in oriental medicine to treat inflammatory diseases. It is well known that ambient particulate matter (PM) is closely related to increased respiratory diseases inducing lung inflammation. AIM: Considering the use of Sargassum horneri in traditional medicine to treat inflammatory diseases, we hypothesized and investigated the use of Sargassum horneri containing polyphenols against PM-induced inflammatory responses. MATERIALS AND METHODS: In this study, we evaluated the impact of PM (majority <2.5 mum in diameter) on deep bronchial penetration ability upon inhalation and a therapeutic approach to mitigate its harmful effects using an ethanol extract of Sargassum horneri, an edible brown algae, containing polyphenols on a type II alveolar epithelial cell line, MLE-12. RESULTS: PM triggered mRNA expression of toll-like receptors (TLRs) TLR2/4/7, and those TLRs were significantly attenuated by Sargassum horneri extract (SHE). SHE further attenuated the phosphorylation of mitogen-activated protein kinase (MAPK) p38, extracellular signal-regulated kinase 1/2 (Erk1/2), and c-Jun NH (2)-terminal kinase (JNK), which were also activated in PM-exposed cells. Altogether, SHE subdued the PM-induced mRNA expression of pro-inflammatory cytokines (interleukin (IL)-1beta, tumor necrosis factor (TNF)-alpha, IL-6) and lung epithelial cell derived-chemokines (IL-8, monocyte chemoattractant protein-1 (MCP-1), and chemokine (C-C motif) ligand 5 (CCL5)). SHE also suppressed the mRNA expression of PM-induced pro-allergic cytokines thymic stromal lymphopoietin (TSLP) and interleukin (IL)-33. Furthermore, we showed that SHE suppressed the MAPK-dependent signaling pathway by attenuating receptor-associated factor (TRAF) 6 activation of proteins MyD88 and TNF. CONCLUSION: Taking all the data together, we suggest that the anti-inflammatory potential of SHE on PM-exposed MLE-12 cells is mediated by the inhibition of PM-triggered downstream signaling along the TLR2/4/7-MyD88-TRAF6 axis of MAPK signaling. CI - Copyright (c) 2020 Elsevier B.V. All rights reserved. FAU - Herath, Kalahe Hewage Iresha Nadeeka Madushani AU - Herath KHINM AD - Interdisciplinary Graduate Program in Advanced Convergence Technology & Science, Jeju National University, Jeju, 63243, Republic of Korea. Electronic address: madushaniherath001@gmail.com. FAU - Kim, Hyo Jin AU - Kim HJ AD - Department of Food Bioengineering, Jeju National University, 102 JeJudaehakro, Jeju, 63243, Republic of Korea. Electronic address: jyouding@gmail.com. FAU - Lee, Ju Hee AU - Lee JH AD - Department of Food Bioengineering, Jeju National University, 102 JeJudaehakro, Jeju, 63243, Republic of Korea. Electronic address: wngml9993@naver.com. FAU - Je, Jun Geon AU - Je JG AD - Department of Marine Life Science, Jeju National University, Jeju, 63243, Republic of Korea. FAU - Yu, Hak-Sun AU - Yu HS AD - Department of Parasitology, Pusan National University School of Medicine, Yangsan, Republic of Korea. Electronic address: hsyu@pusan.ac.kr. FAU - Jeon, You-Jin AU - Jeon YJ AD - Department of Marine Life Science, Jeju National University, Jeju, 63243, Republic of Korea. Electronic address: youjin2014@gmail.com. FAU - Kim, Hyun Jung AU - Kim HJ AD - Department of Food Bioengineering, Jeju National University, 102 JeJudaehakro, Jeju, 63243, Republic of Korea. Electronic address: hyunjkim@jejunu.ac.kr. FAU - Jee, Youngheun AU - Jee Y AD - Interdisciplinary Graduate Program in Advanced Convergence Technology & Science, Jeju National University, Jeju, 63243, Republic of Korea; Department of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University, Jeju, 63243, Republic of Korea. Electronic address: yhjee@jejunu.ac.kr. LA - eng PT - Journal Article DEP - 20200904 PL - Ireland TA - J Ethnopharmacol JT - Journal of ethnopharmacology JID - 7903310 RN - 0 (Anti-Inflammatory Agents) RN - 0 (Myd88 protein, mouse) RN - 0 (Myeloid Differentiation Factor 88) RN - 0 (Particulate Matter) RN - 0 (Polyphenols) RN - 0 (Toll-Like Receptors) SB - IM MH - Animals MH - Anti-Inflammatory Agents/isolation & purification/*pharmacology MH - Cell Line MH - Inflammation/*drug therapy/pathology MH - MAP Kinase Signaling System/drug effects MH - Mice MH - Myeloid Differentiation Factor 88/metabolism MH - Particulate Matter/toxicity MH - Polyphenols/isolation & purification/*pharmacology MH - Sargassum/*chemistry MH - Signal Transduction/drug effects MH - Toll-Like Receptors/metabolism OTO - NOTNLM OT - MyD88 OT - Particulate matter OT - Polyphenols OT - TLR 2 OT - TLR4 OT - TLR7 OT - TSLP EDAT- 2020/09/07 06:00 MHDA- 2021/02/27 06:00 CRDT- 2020/09/06 20:25 PHST- 2020/03/24 00:00 [received] PHST- 2020/08/17 00:00 [revised] PHST- 2020/08/26 00:00 [accepted] PHST- 2020/09/07 06:00 [pubmed] PHST- 2021/02/27 06:00 [medline] PHST- 2020/09/06 20:25 [entrez] AID - S0378-8741(20)33222-0 [pii] AID - 10.1016/j.jep.2020.113340 [doi] PST - ppublish SO - J Ethnopharmacol. 2021 Jan 30;265:113340. doi: 10.1016/j.jep.2020.113340. Epub 2020 Sep 4.