PMID- 32892254
OWN - NLM
STAT- MEDLINE
DCOM- 20210305
LR  - 20220416
IS  - 1432-2048 (Electronic)
IS  - 0032-0935 (Linking)
VI  - 252
IP  - 4
DP  - 2020 Sep 5
TI  - Self-transcriptional repression of the Arabidopsis NAC transcription factor ATAF2 
      and its genetic interaction with phytochrome A in modulating seedling 
      photomorphogenesis.
PG  - 48
LID - 10.1007/s00425-020-03456-5 [doi]
AB  - The NAC transcription factor ATAF2 suppresses its own transcription via 
      self-promoter binding. ATAF2 genetically interacts with the circadian regulator 
      CCA1 and phytochrome A to modulate seedling photomorphogenesis in Arabidopsis 
      thaliana. ATAF2 (ANAC081) is a NAC (NAM, ATAF and CUC) transcription factor (TF) 
      that participates in the regulation of disease resistance, stress tolerance and 
      hormone metabolism in Arabidopsis thaliana. We previously reported that ATAF2 
      promotes Arabidopsis hypocotyl growth in a light-dependent manner via 
      transcriptionally suppressing the brassinosteroid (BR)-inactivating cytochrome 
      P450 genes BAS1 (CYP734A1, formerly CYP72B1) and SOB7 (CYP72C1). Assays using low 
      light intensities suggest that the photoreceptor phytochrome A (PHYA) may play a 
      more critical role in ATAF2-regulated photomorphogenesis than phytochrome B 
      (PHYB) and cryptochrome 1 (CRY1). In addition, ATAF2 is also regulated by the 
      circadian clock. The core circadian TF CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) 
      physically interacts with ATAF2 at the DNA-protein and protein-protein levels, 
      and both differentially suppress BAS1- and SOB7-mediated BR catabolism. In this 
      research, we show that ATAF2 can bind its own promoter as a transcriptional 
      self-repressor. This self-feedback-suppression loop is a typical feature of 
      multiple circadian-regulated genes. Additionally, ATAF2 and CCA1 synergistically 
      suppress seedling photomorphogenesis as reflected by the light-dependent 
      hypocotyl growth analysis of their single and double gene knock-out mutants. 
      Similar fluence-rate response assays using ATAF2 and photoreceptor (PHYB, CRY1 
      and PHYA) knock-out mutants demonstrate that PHYA is required for ATAF2-regulated 
      photomorphogenesis in a wide range of light intensities. Furthermore, disruption 
      of PHYA can suppress the BR-insensitive hypocotyl-growth phenotype of ATAF2 
      loss-of-function seedlings in the light, but not in darkness. Collectively, our 
      results provide a genetic interaction synopsis of the 
      circadian-clock-photomorphogenesis-BR integration node involving ATAF2, CCA1 and 
      PHYA.
FAU - Peng, Hao
AU  - Peng H
AUID- ORCID: 0000-0003-1915-6104
AD  - Department of Crop and Soil Sciences, Washington State University, Pullman, WA, 
      99164, USA.
FAU - Phung, Jessica
AU  - Phung J
AD  - Department of Crop and Soil Sciences, Washington State University, Pullman, WA, 
      99164, USA.
FAU - Zhai, Ying
AU  - Zhai Y
AUID- ORCID: 0000-0001-7997-7794
AD  - Department of Plant Pathology, Washington State University, Pullman, WA, 99164, 
      USA.
FAU - Neff, Michael M
AU  - Neff MM
AUID- ORCID: 0000-0003-2170-5555
AD  - Department of Crop and Soil Sciences, Washington State University, Pullman, WA, 
      99164, USA. mmneff@wsu.edu.
LA  - eng
GR  - 1656265/National Science Foundation/
GR  - 1015621/National Institute of Food and Agriculture/
PT  - Journal Article
DEP - 20200905
PL  - Germany
TA  - Planta
JT  - Planta
JID - 1250576
RN  - 0 (ATAF2 protein, Arabidopsis)
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Phytochrome A)
RN  - 0 (Repressor Proteins)
RN  - 136250-22-1 (Phytochrome B)
SB  - IM
MH  - *Arabidopsis/genetics/growth & development/metabolism
MH  - *Arabidopsis Proteins/genetics/metabolism
MH  - *Gene Expression Regulation, Plant
MH  - Mutation
MH  - *Phytochrome A/metabolism
MH  - Phytochrome B/genetics
MH  - *Plant Development/genetics
MH  - *Repressor Proteins/genetics/metabolism
MH  - Seedlings/genetics
OTO - NOTNLM
OT  - ATAF2
OT  - Brassinosteroid
OT  - CCA1
OT  - Hypocotyl growth
OT  - Photomorphogenesis
OT  - Phytochrome A
EDAT- 2020/09/07 06:00
MHDA- 2021/03/06 06:00
CRDT- 2020/09/06 20:36
PHST- 2020/05/08 00:00 [received]
PHST- 2020/08/27 00:00 [accepted]
PHST- 2020/09/06 20:36 [entrez]
PHST- 2020/09/07 06:00 [pubmed]
PHST- 2021/03/06 06:00 [medline]
AID - 10.1007/s00425-020-03456-5 [pii]
AID - 10.1007/s00425-020-03456-5 [doi]
PST - epublish
SO  - Planta. 2020 Sep 5;252(4):48. doi: 10.1007/s00425-020-03456-5.