PMID- 32894184 OWN - NLM STAT- MEDLINE DCOM- 20210618 LR - 20210618 IS - 1757-6512 (Electronic) IS - 1757-6512 (Linking) VI - 11 IP - 1 DP - 2020 Sep 7 TI - Effect of local anesthetics on viability and differentiation of various adult stem/progenitor cells. PG - 385 LID - 10.1186/s13287-020-01905-2 [doi] LID - 385 AB - BACKGROUND: Local anesthetics (LAs) are widely used to control pain during various clinical treatments. One of the side effects of LAs, cytotoxicity, has been investigated in various cells including stem/progenitor cells. However, our understanding of the effects of LAs on the differentiation capacity of stem/progenitor cells still remains limited. Therefore, a comparative study was conducted to investigate the effects of multiple LAs on viability and multi-lineage differentiation of stem/progenitor cells that originated from various adult tissues. METHOD: Multiple types of stem/progenitor cells, including bone marrow mesenchymal stem/progenitor cells (MSCs), dental pulp stem/progenitor cells (DPSCs), periodontal ligament stem/progenitor cells (PDLSCs), and tendon-derived stem/progenitor cells, were either obtained from a commercial provider or isolated from adult human donors. Lidocaine (LD) and bupivacaine (BP) at various doses (1x, 0.75x, 0.5x, and 0.25x of each physiological dose) were applied to the different stem/progenitor cells for an hour, followed by induction of fibrogenic, chondrogenic, osteogenic, and adipogenic differentiation. Live/dead and MTT assays were performed at 24 h after the LD or BP treatment. At 2 weeks, qRT-PCR was conducted to evaluate the gene expressions associated with differentiation. After 4 weeks, multiple biochemical staining was performed to evaluate matrix deposition. RESULTS: At 24 h after LD or BP treatment, 1x and 0.75x physiological doses of LD and BP showed significant cytotoxicity in all the tested adult stem/progenitor cells. At 0.5x, BP resulted in higher viability than the same dose LD, with variance between cell types. Overall, the gene expressions associated with fibrogenic, chondrogenic, osteogenic, and adipogenic differentiation were attenuated in LD or BP pre-treated stem/progenitor cells, with notable dose-effect and dependence on types. In contrast, certain doses of LD and/or BP were found to increase specific gene expression, depending on the cell types. CONCLUSION: Our data suggest that LAs such as LD and BP affect not only the viability but also the differentiation capacity of adult stem/progenitor cells from various anatomical sites. This study sheds light on stem cell applications for tissue regeneration in which isolation and transplantation of stem cells frequently involve LA administration. FAU - Kim, Young Hoon AU - Kim YH AD - Department of Anesthesiology and Pain Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea. FAU - Park, Ga Young AU - Park GY AD - Regenerative Engineering Laboratory, Center for Dental and Craniofacial Research, Columbia University Irving Medical Center, 630 West 168th Street, VC12-211, New York, NY, 10032, USA. FAU - Rabinovitch, Nechama AU - Rabinovitch N AD - Regenerative Engineering Laboratory, Center for Dental and Craniofacial Research, Columbia University Irving Medical Center, 630 West 168th Street, VC12-211, New York, NY, 10032, USA. FAU - Tarafder, Solaiman AU - Tarafder S AD - Regenerative Engineering Laboratory, Center for Dental and Craniofacial Research, Columbia University Irving Medical Center, 630 West 168th Street, VC12-211, New York, NY, 10032, USA. FAU - Lee, Chang H AU - Lee CH AUID- ORCID: 0000-0002-0081-1191 AD - Regenerative Engineering Laboratory, Center for Dental and Craniofacial Research, Columbia University Irving Medical Center, 630 West 168th Street, VC12-211, New York, NY, 10032, USA. chl2109@cumc.columbia.edu. LA - eng PT - Journal Article PT - Research Support, N.I.H., Extramural DEP - 20200907 PL - England TA - Stem Cell Res Ther JT - Stem cell research & therapy JID - 101527581 RN - 0 (Anesthetics, Local) SB - IM MH - Adult MH - *Anesthetics, Local/toxicity MH - Cell Differentiation MH - Cell Proliferation MH - Cells, Cultured MH - Humans MH - *Mesenchymal Stem Cells MH - Osteogenesis MH - Stem Cells PMC - PMC7487635 OTO - NOTNLM OT - Bupivacaine OT - Lidocaine OT - Local anesthetics OT - Regenerative medicine OT - Stem cells COIS- Authors have no competing interests related to the present study. EDAT- 2020/09/08 06:00 MHDA- 2021/06/22 06:00 PMCR- 2020/09/07 CRDT- 2020/09/07 12:10 PHST- 2020/05/04 00:00 [received] PHST- 2020/08/26 00:00 [accepted] PHST- 2020/08/11 00:00 [revised] PHST- 2020/09/07 12:10 [entrez] PHST- 2020/09/08 06:00 [pubmed] PHST- 2021/06/22 06:00 [medline] PHST- 2020/09/07 00:00 [pmc-release] AID - 10.1186/s13287-020-01905-2 [pii] AID - 1905 [pii] AID - 10.1186/s13287-020-01905-2 [doi] PST - epublish SO - Stem Cell Res Ther. 2020 Sep 7;11(1):385. doi: 10.1186/s13287-020-01905-2.