PMID- 32894647
OWN - NLM
STAT- MEDLINE
DCOM- 20210623
LR  - 20210623
IS  - 1864-0648 (Electronic)
IS  - 1864-063X (Linking)
VI  - 13
IP  - 12
DP  - 2020 Dec
TI  - UnaG as a reporter in adeno-associated virus-mediated gene transfer for 
      biomedical imaging.
PG  - e202000182
LID - 10.1002/jbio.202000182 [doi]
AB  - Adeno-associated virus (AAV) is one of the most common gene transfer vectors, but 
      it has a limited capacity. A smaller fluorescent protein is urgently needed since 
      it is more suitable to act as a reporter in AAV. In this study, a 
      bilirubin-dependent reporter smaller than EGFP, termed UnaG, was found to have 
      the ability to label the neurons of a mouse brain as clearly as EGFP without the 
      addition of exogenous bilirubin. We also found that UnaG's pH tolerance is better 
      than that of EGFP; however, its fluorescence recovery after protonated quenching 
      is not as good as that of EGFP. In addition, UnaG preserved its fluorescence 
      better than EGFP in SeeDB clearing. Taken together, this study demonstrates that 
      UnaG can act as a small intrinsically fluorescent reporter in the mouse brain 
      without an additional ligand, thus providing an alternative over EGFP for 
      AAV-mediated neuron labeling in mammals.
CI  - (c) 2020 Wiley-VCH GmbH.
FAU - Liu, Yurong
AU  - Liu Y
AD  - Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for 
      Optoelectronics-Huazhong University of Science and Technology, Wuhan, China.
AD  - MOE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for 
      Biomedical Engineering, School of Engineering Sciences, Huazhong University of 
      Science and Technology, Wuhan, China.
FAU - Zhang, Huimin
AU  - Zhang H
AD  - Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for 
      Optoelectronics-Huazhong University of Science and Technology, Wuhan, China.
AD  - MOE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for 
      Biomedical Engineering, School of Engineering Sciences, Huazhong University of 
      Science and Technology, Wuhan, China.
FAU - Chen, Ruixi
AU  - Chen R
AD  - Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for 
      Optoelectronics-Huazhong University of Science and Technology, Wuhan, China.
AD  - MOE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for 
      Biomedical Engineering, School of Engineering Sciences, Huazhong University of 
      Science and Technology, Wuhan, China.
FAU - Wu, Ying
AU  - Wu Y
AD  - Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for 
      Optoelectronics-Huazhong University of Science and Technology, Wuhan, China.
AD  - MOE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for 
      Biomedical Engineering, School of Engineering Sciences, Huazhong University of 
      Science and Technology, Wuhan, China.
FAU - Yang, Xiong
AU  - Yang X
AD  - Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for 
      Optoelectronics-Huazhong University of Science and Technology, Wuhan, China.
AD  - MOE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for 
      Biomedical Engineering, School of Engineering Sciences, Huazhong University of 
      Science and Technology, Wuhan, China.
FAU - Liu, Xiuli
AU  - Liu X
AD  - Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for 
      Optoelectronics-Huazhong University of Science and Technology, Wuhan, China.
AD  - MOE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for 
      Biomedical Engineering, School of Engineering Sciences, Huazhong University of 
      Science and Technology, Wuhan, China.
FAU - Zeng, Shaoqun
AU  - Zeng S
AD  - Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for 
      Optoelectronics-Huazhong University of Science and Technology, Wuhan, China.
AD  - MOE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for 
      Biomedical Engineering, School of Engineering Sciences, Huazhong University of 
      Science and Technology, Wuhan, China.
FAU - Guo, Wenyan
AU  - Guo W
AUID- ORCID: 0000-0002-4574-4092
AD  - Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for 
      Optoelectronics-Huazhong University of Science and Technology, Wuhan, China.
AD  - MOE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for 
      Biomedical Engineering, School of Engineering Sciences, Huazhong University of 
      Science and Technology, Wuhan, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200915
PL  - Germany
TA  - J Biophotonics
JT  - Journal of biophotonics
JID - 101318567
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - *Dependovirus/genetics
MH  - Genetic Therapy
MH  - *Genetic Vectors/genetics
MH  - Green Fluorescent Proteins/genetics
MH  - Mice
MH  - Neurons
OTO - NOTNLM
OT  - UnaG
OT  - adeno-associated viruses
OT  - ligand
OT  - reporter
OT  - tissue optical clearing
EDAT- 2020/09/08 06:00
MHDA- 2021/06/24 06:00
CRDT- 2020/09/07 12:14
PHST- 2020/05/11 00:00 [received]
PHST- 2020/08/21 00:00 [revised]
PHST- 2020/09/02 00:00 [accepted]
PHST- 2020/09/08 06:00 [pubmed]
PHST- 2021/06/24 06:00 [medline]
PHST- 2020/09/07 12:14 [entrez]
AID - 10.1002/jbio.202000182 [doi]
PST - ppublish
SO  - J Biophotonics. 2020 Dec;13(12):e202000182. doi: 10.1002/jbio.202000182. Epub 
      2020 Sep 15.