PMID- 32894933
OWN - NLM
STAT- MEDLINE
DCOM- 20201105
LR  - 20201105
IS  - 0393-974X (Print)
IS  - 0393-974X (Linking)
VI  - 34
IP  - 4
DP  - 2020 Jul-Aug
TI  - MiR-424 Inhibits neuronal apoptosis in rats with cerebral infarction through 
      regulating TGF-beta1/Smad3 signaling pathway.
PG  - 1285-1296
LID - 10.23812/20-176-A [doi]
AB  - It has been reported that micro ribonucleic acid (miR)-424 is an important 
      molecule in cerebral ischemia. However, the precise mechanism of action and 
      biological effects of miR-424 remain to be further explored. miR-424 mimic and 
      miR-424 inhibitor were injected via the caudal vein in rats, and the effect of 
      miR-424 expression on brain tissue damage induced by middle cerebral artery 
      occlusion (MCAO) was detected. The miR-424 mimic-induced changes in genomic 
      levels were detected via the gene chip assay, and the signaling pathways 
      regulated by miR-424 and its potential targets were explored combined with target 
      prediction. Then the effect of miR-424 mimic on apoptosis of PC12 cells induced 
      by oxygen-glucose deprivation (OGD) was determined using Annexin V/PI assay. 
      Finally, drosophila mothers against decapentaplegic protein 7 (Smad7) was 
      overexpressed to further verify the mechanism of action of miR-424 mimic. 
      Compared with that in the sham group, the expression of miR-424 in brain tissues 
      significantly declined in the model group. The results of 
      2,3,5-triphenyltetrazolium chloride (TTC) staining and terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) assay revealed that the 
      miR-424 mimic obviously reduced the cerebral infarction area and apoptosis level 
      of brain tissues, while the miR-424 inhibitor obviously increased the cerebral 
      infarction area and apoptosis level of brain tissues. It was found, using 
      bioinformatics and KEGG enrichment analysis, that differentially expressed genes 
      induced by miR-424 were significantly enriched in the transforming growth 
      factor-beta (TGF-beta) signaling pathway. According to the results of reverse 
      transcription-quantitative polymerase chain reaction (RT-qPCR) and Western 
      blotting, the miR-424 mimic could evidently lower the expression of Smad7, thus 
      activating the TGF-beta1/Smad3 signaling pathway. Overexpression of Smad7 could 
      weaken the protective effect of miR-424 mimic on ischemic-hypoxic cells. 
      Increasing the expression of miR-424 can inhibit Smad7 to activate the 
      TGF-beta1/Smad3 signaling pathway, thereby exerting a protective effect against the 
      brain tissue damage induced by MCAO.
CI  - Copyright 2020 Biolife Sas. www.biolifesas.org.
FAU - Gui, S H
AU  - Gui SH
AD  - Department of Neurology, Affiliated Hospital of Jiangnan University/The Third 
      People's Hospital of Wuxi, Wuxi, China.
FAU - Wang, Y M
AU  - Wang YM
AD  - Department of Neurology, Affiliated Hospital of Jiangnan University/The Third 
      People's Hospital of Wuxi, Wuxi, China.
FAU - Fan, C Y
AU  - Fan CY
AD  - Department of Neurology, Affiliated Hospital of Jiangnan University/The Third 
      People's Hospital of Wuxi, Wuxi, China.
FAU - Han, L K
AU  - Han LK
AD  - Department of Neurology, Affiliated Hospital of Jiangnan University/The Third 
      People's Hospital of Wuxi, Wuxi, China.
FAU - Wang, T G
AU  - Wang TG
AD  - Department of Neurology, Affiliated Hospital of Jiangnan University/The Third 
      People's Hospital of Wuxi, Wuxi, China.
FAU - Di, M Q
AU  - Di MQ
AD  - Department of Neurology, Affiliated Hospital of Jiangnan University/The Third 
      People's Hospital of Wuxi, Wuxi, China.
FAU - Gu, J Q
AU  - Gu JQ
AD  - Department of Neurology, Affiliated Hospital of Jiangnan University/The Third 
      People's Hospital of Wuxi, Wuxi, China.
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - J Biol Regul Homeost Agents
JT  - Journal of biological regulators and homeostatic agents
JID - 8809253
RN  - 0 (MicroRNAs)
RN  - 0 (Smad3 Protein)
RN  - 0 (Smad3 protein, rat)
RN  - 0 (Transforming Growth Factor beta1)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - MicroRNAs/genetics
MH  - Neurons
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Signal Transduction
MH  - Smad3 Protein
MH  - Transforming Growth Factor beta1/genetics
OTO - NOTNLM
OT  - TGF-beta1/Smad3 signaling pathway
OT  - apoptosis
OT  - cerebral infarction
OT  - miR-424
OT  - neurons
EDAT- 2020/09/09 06:00
MHDA- 2020/11/06 06:00
CRDT- 2020/09/08 04:19
PHST- 2020/09/08 04:19 [entrez]
PHST- 2020/09/09 06:00 [pubmed]
PHST- 2020/11/06 06:00 [medline]
AID - 36 [pii]
AID - 10.23812/20-176-A [doi]
PST - ppublish
SO  - J Biol Regul Homeost Agents. 2020 Jul-Aug;34(4):1285-1296. doi: 
      10.23812/20-176-A.