PMID- 32896319
OWN - NLM
STAT- MEDLINE
DCOM- 20210706
LR  - 20210706
IS  - 0091-679X (Print)
IS  - 0091-679X (Linking)
VI  - 160
DP  - 2020
TI  - Super-resolution imaging of microtubules in Medicago sativa.
PG  - 237-251
LID - S0091-679X(20)30032-7 [pii]
LID - 10.1016/bs.mcb.2020.03.004 [doi]
AB  - Study of microtubules on cellular and subcellular levels is compromised by 
      limited resolution of conventional fluorescence microscopy. However, it is 
      possible to improve Abbe's diffraction-limited resolution by employment of 
      super-resolution microscopy methods. Two of them, described herein, are 
      structured-illumination microscopy (SIM) and Airyscan laser scanning microscopy 
      (AM). Both methods allow high-resolution imaging of cortical microtubules in 
      plant cells, thus contributing to the current knowledge on plant morphogenesis, 
      growth and development. Both SIM and AM provide certain advantages and 
      characteristic features, which are described here. We present immunofluorescence 
      localization methods for microtubules in fixed plant cells achieving high signal 
      efficiency, superb sample stability and sub-diffraction resolution. These 
      protocols were developed for whole-mount immunolabeling of root samples of legume 
      crop species Medicago sativa. They also contain tips for optimal sample 
      preparation of plants germinated from seeds as well as plantlets regenerated from 
      somatic embryos in vitro. We describe in detail all steps of optimized protocols 
      for sample preparation, microtubule immunolabeling and super-resolution imaging.
CI  - (c) 2020 Elsevier Inc. All rights reserved.
FAU - Ticha, Michaela
AU  - Ticha M
AD  - Department of Cell Biology, Centre of the Region Hana for Biotechnological and 
      Agricultural Research, Faculty of Science, Palacky University Olomouc, Olomouc, 
      Czech Republic.
FAU - Hlavackova, Katerina
AU  - Hlavackova K
AD  - Department of Cell Biology, Centre of the Region Hana for Biotechnological and 
      Agricultural Research, Faculty of Science, Palacky University Olomouc, Olomouc, 
      Czech Republic.
FAU - Hrbackova, Miroslava
AU  - Hrbackova M
AD  - Department of Cell Biology, Centre of the Region Hana for Biotechnological and 
      Agricultural Research, Faculty of Science, Palacky University Olomouc, Olomouc, 
      Czech Republic.
FAU - Ovecka, Miroslav
AU  - Ovecka M
AD  - Department of Cell Biology, Centre of the Region Hana for Biotechnological and 
      Agricultural Research, Faculty of Science, Palacky University Olomouc, Olomouc, 
      Czech Republic.
FAU - Samajova, Olga
AU  - Samajova O
AD  - Department of Cell Biology, Centre of the Region Hana for Biotechnological and 
      Agricultural Research, Faculty of Science, Palacky University Olomouc, Olomouc, 
      Czech Republic.
FAU - Samaj, Jozef
AU  - Samaj J
AD  - Department of Cell Biology, Centre of the Region Hana for Biotechnological and 
      Agricultural Research, Faculty of Science, Palacky University Olomouc, Olomouc, 
      Czech Republic. Electronic address: jozef.samaj@upol.cz.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200429
PL  - United States
TA  - Methods Cell Biol
JT  - Methods in cell biology
JID - 0373334
SB  - IM
MH  - Imaging, Three-Dimensional/*methods
MH  - Medicago sativa/cytology/*metabolism
MH  - Microscopy, Confocal
MH  - Microtubules/*metabolism
MH  - Plant Epidermis/cytology
MH  - Plant Roots/cytology
MH  - Seedlings/metabolism
OTO - NOTNLM
OT  - Airyscan microscopy
OT  - CLSM
OT  - Immunofluorescence labeling
OT  - Medicago sativa
OT  - Microtubules
OT  - Structured-illumination microscopy
EDAT- 2020/09/09 06:00
MHDA- 2021/07/07 06:00
CRDT- 2020/09/08 08:46
PHST- 2020/09/08 08:46 [entrez]
PHST- 2020/09/09 06:00 [pubmed]
PHST- 2021/07/07 06:00 [medline]
AID - S0091-679X(20)30032-7 [pii]
AID - 10.1016/bs.mcb.2020.03.004 [doi]
PST - ppublish
SO  - Methods Cell Biol. 2020;160:237-251. doi: 10.1016/bs.mcb.2020.03.004. Epub 2020 
      Apr 29.