PMID- 32897396
OWN - NLM
STAT- MEDLINE
DCOM- 20210602
LR  - 20220418
IS  - 1432-203X (Electronic)
IS  - 0721-7714 (Linking)
VI  - 39
IP  - 12
DP  - 2020 Dec
TI  - Localization and characterization of Citrus centromeres by combining half-tetrad 
      analysis and CenH3-associated sequence profiling.
PG  - 1609-1622
LID - 10.1007/s00299-020-02587-z [doi]
AB  - The physical locations of citrus centromere are revealed by combining genetic and 
      immunological assays for the first time and nine citrus centromere-specific 
      markers for cytogenetics are mined. Centromere localization is challenging, 
      because highly redundant repetitive sequences in centromeric regions make 
      sequence assembly difficult. Although several citrus genomes have been released, 
      the centromeric regions and their characteristics remain to be elucidated. Here, 
      we mapped citrus centromeres through half-tetrad analysis (HTA) that included the 
      genotyping of 54 tetraploid hybrids derived from 2n megagametophytes of Nadorcott 
      tangor with 212 single nucleotide polymorphism (SNP) markers. The sizes of 
      centromeric regions, which estimated based on the heterozygosity restitution rate 
      pattern along the chromosomes, ranged from 1.12 to 18.19 Mb. We also profiled the 
      binding sequences with the centromere-specific histone variant CenH3 by chromatin 
      immunoprecipitation sequencing (ChIP-seq). Based on the positions of the top ten 
      CenH3-enriched contigs, the sizes of centromeric regions were estimated to range 
      from 0.01 to 7.60 Mb and were either adjacent to or included in the centromeric 
      regions identified by HTA. We used DNA probes from two repeats selected from 
      the centromeric regions and seven CenH3-binding centromeric repeats to verify 
      centromeric locations by fluorescence in situ hybridization (FISH). Centromere 
      localization in citrus will contribute to the mining of 
      centromeric/pericentromeric markers, thus to facilitate the rapid identification 
      of mechanisms underlying 2n gamete formation and serve the polyploidy breeding.
FAU - Xia, Qiang-Ming
AU  - Xia QM
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 
      430070, China.
FAU - Miao, Lu-Ke
AU  - Miao LK
AD  - College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 
      430070, China.
FAU - Xie, Kai-Dong
AU  - Xie KD
AUID- ORCID: 0000-0003-4242-2423
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 
      430070, China. xiekaidong@mail.hzau.edu.cn.
FAU - Yin, Zhao-Ping
AU  - Yin ZP
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 
      430070, China.
FAU - Wu, Xiao-Meng
AU  - Wu XM
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 
      430070, China.
FAU - Chen, Chun-Li
AU  - Chen CL
AD  - College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 
      430070, China.
FAU - Grosser, Jude W
AU  - Grosser JW
AD  - Citrus Research and Education Center, University of Florida, 700 Experiment 
      Station Road, Lake Alfred, FL, 33850, USA.
FAU - Guo, Wen-Wu
AU  - Guo WW
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 
      430070, China.
LA  - eng
GR  - 2018YFD1000200/the National Key R&D Program of China/
GR  - 31820103011/National Natural Science Foundation of China/
GR  - 31701873/National Natural Science Foundation of China/
GR  - 31530065/National Natural Science Foundation of China/
GR  - 2662018PY007/Fundamental Research Funds for the Central Universities (CN)/
PT  - Journal Article
DEP - 20200908
PL  - Germany
TA  - Plant Cell Rep
JT  - Plant cell reports
JID - 9880970
SB  - IM
MH  - Antibody Specificity
MH  - Centromere/*genetics
MH  - Chromatin Immunoprecipitation Sequencing
MH  - Citrus/*genetics
MH  - Cytogenetics/*methods
MH  - Genes, Plant/immunology
MH  - Genotyping Techniques/methods
MH  - In Situ Hybridization, Fluorescence
MH  - Polymorphism, Single Nucleotide
MH  - Tetraploidy
OTO - NOTNLM
OT  - 2n megagametophyte
OT  - CenH3
OT  - Centromere location
OT  - Chromatin immunoprecipitation
OT  - Citrus
EDAT- 2020/09/09 06:00
MHDA- 2021/06/03 06:00
CRDT- 2020/09/08 12:14
PHST- 2020/06/07 00:00 [received]
PHST- 2020/08/26 00:00 [accepted]
PHST- 2020/09/09 06:00 [pubmed]
PHST- 2021/06/03 06:00 [medline]
PHST- 2020/09/08 12:14 [entrez]
AID - 10.1007/s00299-020-02587-z [pii]
AID - 10.1007/s00299-020-02587-z [doi]
PST - ppublish
SO  - Plant Cell Rep. 2020 Dec;39(12):1609-1622. doi: 10.1007/s00299-020-02587-z. Epub 
      2020 Sep 8.