PMID- 32897801
OWN - NLM
STAT- MEDLINE
DCOM- 20211207
LR  - 20211214
IS  - 1551-4005 (Electronic)
IS  - 1538-4101 (Print)
IS  - 1551-4005 (Linking)
VI  - 19
IP  - 20
DP  - 2020 Oct
TI  - Knockdown of circular RNA circMAT2B reduces oxygen-glucose deprivation-induced 
      inflammatory injury in H9c2 cells through up-regulating miR-133.
PG  - 2622-2630
LID - 10.1080/15384101.2020.1814025 [doi]
AB  - Myocardial infarction (MI) is the main cause of morbidity and mortality. 
      Reperfusion ways can cause damage to cardiomyocytes. CircMAT2B, a novel circRNA, 
      takes positive roles in regulating glucose metabolism under hypoxia. Therefore, 
      we aimed to explore the effects of circMAT2B on MI. Oxygen-glucose deprivation 
      (OGD)-induced H9c2 cell model was employed to stimulate MI. Ex-circMAT2B, 
      si-circMAT2B, miR-133 inhibitor and relative control were transfected into H9c2 
      cells. qRT-PCR was employed to examine levels of circMAT2B and miR-133. Cell 
      activity, apoptosis, ROS generation and release of inflammatory factors were 
      assessed by CCK-8, flow cytometry, ROS species assay kit and ELISA, respectively. 
      Moreover, the expression of apoptosis-related and pathway-related factors was 
      detected through western blot analysis. The results showed that circMAT2B 
      expression was notably up-regulated by OGD treatment. Moreover, circMAT2B 
      knockdown could effectively decrease OGD-induced the increasing of apoptosis, ROS 
      generation and the expression of IL-1beta, IL-6 and TNF-alpha. Besides, miR-133 was 
      positively regulated by si-circMAT2B. CircMAT2B knockdown attenuated OGD-induced 
      H9c2 cell damage and alleviated OGD-induced the inhibition of PI3K/AKT and 
      Raf/MEK/ERK pathways through up-regulating miR-133. In brief, circMAT2B knockdown 
      works as an inflammatory inhibitor in OGD-induced H9c2 cells inflammatory injury 
      through up-regulating miR-133.
FAU - Zhu, Yanhui
AU  - Zhu Y
AD  - Department of Cardiac Surgery, Shandong Provincial Hospital Affiliated to 
      Shandong University , Jinan, Shandong, China.
FAU - Zou, Chengwei
AU  - Zou C
AD  - Department of Cardiac Surgery, Shandong Provincial Hospital Affiliated to 
      Shandong University , Jinan, Shandong, China.
FAU - Jia, Yanting
AU  - Jia Y
AD  - Department of Cardiac Surgery, Shandong Provincial Hospital Affiliated to 
      Shandong University , Jinan, Shandong, China.
FAU - Zhang, Haizhou
AU  - Zhang H
AD  - Department of Cardiac Surgery, Shandong Provincial Hospital Affiliated to 
      Shandong University , Jinan, Shandong, China.
FAU - Ma, Xiaochun
AU  - Ma X
AD  - Department of Cardiac Surgery, Shandong Provincial Hospital Affiliated to 
      Shandong University , Jinan, Shandong, China.
FAU - Zhang, Jun
AU  - Zhang J
AUID- ORCID: 0000-0002-2267-0182
AD  - Department of Cardiac Surgery, Shandong Provincial Hospital Affiliated to 
      Shandong First Medical University , Jinan, Shandong, China.
LA  - eng
PT  - Journal Article
DEP - 20200908
PL  - United States
TA  - Cell Cycle
JT  - Cell cycle (Georgetown, Tex.)
JID - 101137841
RN  - 0 (MIRN133 microRNA, rat)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Circular)
RN  - 0 (RNA, Long Noncoding)
RN  - IY9XDZ35W2 (Glucose)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Apoptosis/genetics
MH  - Cell Line
MH  - Glucose/*metabolism
MH  - Inflammation/*genetics/*metabolism
MH  - MicroRNAs/*genetics
MH  - Myocardial Infarction/genetics/metabolism
MH  - Myocytes, Cardiac/metabolism
MH  - Oxygen/*metabolism
MH  - RNA, Circular/*genetics
MH  - RNA, Long Noncoding/genetics
MH  - Rats
MH  - Transcriptional Activation/genetics
MH  - Up-Regulation/*genetics
PMC - PMC7644149
OTO - NOTNLM
OT  - Circular RNA circMAT2B
OT  - inflammatory injury
OT  - miR-133
OT  - oxygen-glucose deprivation
COIS- The authors declare that they have no conflict of interest.
EDAT- 2020/09/09 06:00
MHDA- 2021/12/15 06:00
PMCR- 2021/09/08
CRDT- 2020/09/08 17:11
PHST- 2020/09/09 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
PHST- 2020/09/08 17:11 [entrez]
PHST- 2021/09/08 00:00 [pmc-release]
AID - 1814025 [pii]
AID - 10.1080/15384101.2020.1814025 [doi]
PST - ppublish
SO  - Cell Cycle. 2020 Oct;19(20):2622-2630. doi: 10.1080/15384101.2020.1814025. Epub 
      2020 Sep 8.