PMID- 32898329
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211211
IS  - 2001-1326 (Print)
IS  - 2001-1326 (Electronic)
IS  - 2001-1326 (Linking)
VI  - 10
IP  - 4
DP  - 2020 Aug
TI  - Mir-27a-3p attenuates bronchiolitis obliterans in vivo via the regulation of 
      dendritic cells' maturation and the suppression of myofibroblasts' 
      differentiation.
PG  - e140
LID - 10.1002/ctm2.140 [doi]
LID - e140
AB  - Bronchiolitis obliterans (BO), is a chronic rejection phenotype characterized by 
      chronic small airway fibrous obliteration, hinders the patients who suffer from 
      lung transplanting for surviving longer. Cell-based therapies using dendritic 
      cells (DCs) and T regulatory cells (Tregs) have been developed to regulate 
      allograft rejection, and to induce and maintain immune tolerance. In the present 
      study, the effects of mir-27a-3p on regulating DCs as well as resulting effects 
      on BO attenuation have been investigated. According to our reporter assays, the 
      potential targets of mir-27a-3p were Smad2, sprouty2, and Smad4, respectively. 
      Furthermore, sprouty2 inhibition by mir-27-3p indirectly activated extracellular 
      regulated protein kinases (ERK) and increased IL-10 production in DCs. This led 
      to a positive feedback loop that maintained the immature state of DCs via 
      IL-10/JAK/STAT3 pathway, and caused an increase in Foxp3(+) CD4(+) T cells amount 
      as well as TGF-beta level. Furthermore, mir-27a-3p regulated TGF-beta function, 
      inhibited TGF-beta/Smad pathway, and suppressed myofibroblast differentiation 
      through influencing the function of Smad2 and Smad4. In short, the study 
      indicated the effect of mir-27a-3p on suppressing DC maturation, which implicated 
      the potential clinical application in treating postlung transplant BO.
CI  - (c) 2020 The Authors. Clinical and Translational Medicine published by John Wiley & 
      Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics.
FAU - Dong, Ming
AU  - Dong M
AUID- ORCID: 0000-0002-0296-3519
AD  - Department of Lung Cancer Surgery, Tianjin Medical University General Hospital, 
      Tianjin, P. R. China.
FAU - Wang, Xin
AU  - Wang X
AD  - Department of Pediatric Surgery, Tianjin Children's Hospital, Tianjin, P. R. 
      China.
FAU - L, Tong
AU  - L T
AD  - Department of Lung Cancer Surgery, Tianjin Medical University General Hospital, 
      Tianjin, P. R. China.
FAU - Wang, Jing
AU  - Wang J
AD  - Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, 
      Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, 
      Tianjin, P. R. China.
FAU - Yang, Yunwei
AU  - Yang Y
AD  - School of Basic Medical Sciences, Tianjin Medical University, Tianjin, P. R. 
      China.
FAU - Liu, Yi
AU  - Liu Y
AD  - School of Basic Medical Sciences, Tianjin Medical University, Tianjin, P. R. 
      China.
FAU - Jing, Yaqing
AU  - Jing Y
AD  - School of Basic Medical Sciences, Tianjin Medical University, Tianjin, P. R. 
      China.
FAU - Zhao, Honglin
AU  - Zhao H
AD  - Department of Lung Cancer Surgery, Tianjin Medical University General Hospital, 
      Tianjin, P. R. China.
FAU - Chen, Jun
AU  - Chen J
AD  - Department of Lung Cancer Surgery, Tianjin Medical University General Hospital, 
      Tianjin, P. R. China.
LA  - eng
GR  - 18JCYBJC92600/Natural Science Foundation of Tianjin City/
GR  - 81600073/National Natural Science Foundation of China/
GR  - 320.6750.18157/Wu Jieping Medical Foundation/
GR  - HZB-20181119-7/Bethune Ethicon Excellent Surgery Foundation/
PT  - Journal Article
PL  - United States
TA  - Clin Transl Med
JT  - Clinical and translational medicine
JID - 101597971
EIN - Clin Transl Med. 2021 Jun;11(6):e433. PMID: 34185434
PMC - PMC7423186
OTO - NOTNLM
OT  - bronchiolitis obliterans
OT  - dendritic cells
OT  - lung transplantation
OT  - mir-27a-3p
COIS- The authors declare that they have no conflict of interest.
EDAT- 2020/09/09 06:00
MHDA- 2020/09/09 06:01
PMCR- 2020/08/12
CRDT- 2020/09/08 17:13
PHST- 2020/05/05 00:00 [received]
PHST- 2020/07/16 00:00 [revised]
PHST- 2020/07/17 00:00 [accepted]
PHST- 2020/09/08 17:13 [entrez]
PHST- 2020/09/09 06:00 [pubmed]
PHST- 2020/09/09 06:01 [medline]
PHST- 2020/08/12 00:00 [pmc-release]
AID - CTM2140 [pii]
AID - 10.1002/ctm2.140 [doi]
PST - ppublish
SO  - Clin Transl Med. 2020 Aug;10(4):e140. doi: 10.1002/ctm2.140.