PMID- 32899112
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201028
IS  - 2075-4426 (Print)
IS  - 2075-4426 (Electronic)
IS  - 2075-4426 (Linking)
VI  - 10
IP  - 3
DP  - 2020 Sep 3
TI  - Induction of Micronuclei in Cervical Cancer Treated with Radiotherapy.
LID - 10.3390/jpm10030110 [doi]
LID - 110
AB  - Micronuclei (MN) trigger antitumor immune responses via the cyclic GMP-AMP 
      synthase-signaling effector stimulator of interferon genes (cGAS-STING) pathway. 
      Radiotherapy induces MN in peripheral blood lymphocytes. However, data for solid 
      tumors are lacking. Here, we analyzed MN post-radiotherapy in solid tumor 
      samples. Tumor biopsy specimens were obtained from seven prospectively recruited 
      patients with cervical cancer, before treatment and after receiving radiotherapy 
      at a dose of 10 Gy (in five fractions). The samples were stained with 
      4',6-diamidino-2-phenylindole dihydrochloride, and 200 nuclei per sample were 
      randomly identified and assessed for the presence of MN or apoptosis, based on 
      nuclear morphology. The median number of MN-harboring nuclei was significantly 
      greater in samples from patients treated with radiotherapy than in pre-treatment 
      samples (151 (range, 16-327) versus 28 (range, 0-61); p = 0.015). No significant 
      differences in the number of apoptotic nuclei were observed between pre-treatment 
      and 10 Gy samples (5 (range, 0-30) versus 12 (range, 2-30); p = 0.30). This is 
      the first report to demonstrate MN induction by radiotherapy in solid tumors. The 
      results provide clinical evidence of the activation of antitumor immune responses 
      by radiotherapy.
FAU - Kobayashi, Daijiro
AU  - Kobayashi D
AUID- ORCID: 0000-0001-8887-9520
AD  - Department of Radiation Oncology, Gunma Prefectural Cancer Center, Gunma 
      373-8550, Japan.
FAU - Oike, Takahiro
AU  - Oike T
AD  - Department of Radiation Oncology, Gunma University Graduate School of Medicine, 
      Gunma 371-8511, Japan.
FAU - Murata, Kazutoshi
AU  - Murata K
AD  - QST Hospital, National Institute for Quantum and Radiological Science and 
      Technology, Chiba 263-8555, Japan.
FAU - Irie, Daisuke
AU  - Irie D
AD  - Department of Radiation Oncology, Gunma University Graduate School of Medicine, 
      Gunma 371-8511, Japan.
FAU - Hirota, Yuka
AU  - Hirota Y
AD  - Department of Radiation Oncology, Gunma University Graduate School of Medicine, 
      Gunma 371-8511, Japan.
FAU - Sato, Hiro
AU  - Sato H
AD  - Department of Radiation Oncology, Gunma University Graduate School of Medicine, 
      Gunma 371-8511, Japan.
FAU - Shibata, Atsushi
AU  - Shibata A
AUID- ORCID: 0000-0001-8912-6977
AD  - Gunma University Initiative for Advanced Research (GIAR), Gunma University, Gunma 
      371-8511, Japan.
FAU - Ohno, Tatsuya
AU  - Ohno T
AUID- ORCID: 0000-0002-6703-3144
AD  - Department of Radiation Oncology, Gunma University Graduate School of Medicine, 
      Gunma 371-8511, Japan.
AD  - Gunma University Heavy Ion Medical Center, Gunma 371-8511, Japan.
LA  - eng
PT  - Journal Article
DEP - 20200903
PL  - Switzerland
TA  - J Pers Med
JT  - Journal of personalized medicine
JID - 101602269
PMC - PMC7563241
OTO - NOTNLM
OT  - STING
OT  - abscopal effect
OT  - cGAS
OT  - immunotherapy
OT  - micronuclei
OT  - radiotherapy
OT  - uterine cervical cancer
COIS- The authors declare no conflict of interest.
EDAT- 2020/09/10 06:00
MHDA- 2020/09/10 06:01
PMCR- 2020/09/01
CRDT- 2020/09/09 01:02
PHST- 2020/08/11 00:00 [received]
PHST- 2020/08/28 00:00 [revised]
PHST- 2020/09/01 00:00 [accepted]
PHST- 2020/09/09 01:02 [entrez]
PHST- 2020/09/10 06:00 [pubmed]
PHST- 2020/09/10 06:01 [medline]
PHST- 2020/09/01 00:00 [pmc-release]
AID - jpm10030110 [pii]
AID - jpm-10-00110 [pii]
AID - 10.3390/jpm10030110 [doi]
PST - epublish
SO  - J Pers Med. 2020 Sep 3;10(3):110. doi: 10.3390/jpm10030110.