PMID- 32899126
OWN - NLM
STAT- MEDLINE
DCOM- 20210325
LR  - 20210325
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 21
IP  - 17
DP  - 2020 Sep 3
TI  - Impact of Human Cytomegalovirus and Human Herpesvirus 6 Infection on the 
      Expression of Factors Associated with Cell Fibrosis and Apoptosis: Clues for 
      Implication in Systemic Sclerosis Development.
LID - 10.3390/ijms21176397 [doi]
LID - 6397
AB  - Systemic sclerosis (SSc) is a severe autoimmune disorder characterized by 
      vasculopathy and multi-organ fibrosis; its etiology and pathogenesis are still 
      largely unknown. Herpesvirus infections, particularly by human cytomegalovirus 
      (HCMV) and human herpesvirus 6 (HHV-6), have been suggested among triggers of the 
      disease based on virological and immunological observations. However, the direct 
      impact of HCMV and/or HHV-6 infection on cell fibrosis and apoptosis at the cell 
      microenvironment level has not yet been clarified. Thus, this study aimed to 
      investigate the effects of HCMV and HHV-6 infection on the induction of 
      pro-fibrosis or pro-apoptosis conditions in primary human dermal fibroblasts, one 
      of the relevant SSc target cells. The analysis, performed by microarray in in 
      vitro HCMV- or HHV-6-infected vs. uninfected cells, using specific panels for the 
      detection of the main cellular factors associated with fibrosis or apoptosis, 
      showed that both viruses significantly modified the expression of at least 30 
      pro-fibrotic and 20 pro-apoptotic factors. Notably, several recognized 
      pro-fibrotic factors were highly induced, and most of them were reported to be 
      involved in vivo in the multifactorial and multistep pathogenic process of SSc, 
      thus suggesting a potential role of both HCMV and HHV-6.
FAU - Arcangeletti, Maria-Cristina
AU  - Arcangeletti MC
AD  - Department of Medicine and Surgery, Unit of Virology, University-Hospital of 
      Parma, University of Parma, 43126 Parma, Italy.
FAU - D'Accolti, Maria
AU  - D'Accolti M
AUID- ORCID: 0000-0003-1937-5534
AD  - Department of Chemical and Pharmaceutical Sciences, Section of Microbiology and 
      Medical Genetics, University of Ferrara, 44121 Ferrara, Italy.
FAU - Maccari, Clara
AU  - Maccari C
AD  - Department of Medicine and Surgery, Unit of Virology, University-Hospital of 
      Parma, University of Parma, 43126 Parma, Italy.
FAU - Soffritti, Irene
AU  - Soffritti I
AUID- ORCID: 0000-0002-5274-295X
AD  - Department of Chemical and Pharmaceutical Sciences, Section of Microbiology and 
      Medical Genetics, University of Ferrara, 44121 Ferrara, Italy.
FAU - Conto, Flora De
AU  - Conto F
AD  - Department of Medicine and Surgery, Unit of Virology, University-Hospital of 
      Parma, University of Parma, 43126 Parma, Italy.
FAU - Chezzi, Carlo
AU  - Chezzi C
AD  - Department of Medicine and Surgery, Unit of Virology, University-Hospital of 
      Parma, University of Parma, 43126 Parma, Italy.
FAU - Calderaro, Adriana
AU  - Calderaro A
AD  - Department of Medicine and Surgery, Unit of Virology, University-Hospital of 
      Parma, University of Parma, 43126 Parma, Italy.
FAU - Ferri, Clodoveo
AU  - Ferri C
AD  - Department of Medical and Surgical Sciences for Children and Adults, Rheumatology 
      Unit, University-Hospital Policlinico of Modena, University of Modena and Reggio 
      Emilia, 41121 Modena, Italy.
FAU - Caselli, Elisabetta
AU  - Caselli E
AUID- ORCID: 0000-0001-6048-9141
AD  - Department of Chemical and Pharmaceutical Sciences, Section of Microbiology and 
      Medical Genetics, University of Ferrara, 44121 Ferrara, Italy.
LA  - eng
GR  - D92F16000490005/Ministero dell'Istruzione, dell'Universita e della Ricerca/
GR  - 2015YZB22C/Ministero dell'Istruzione, dell'Universita e della Ricerca/
PT  - Journal Article
DEP - 20200903
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Apoptosis Regulatory Proteins)
SB  - IM
MH  - *Apoptosis
MH  - Apoptosis Regulatory Proteins/*metabolism
MH  - Cells, Cultured
MH  - Cytomegalovirus/isolation & purification
MH  - Cytomegalovirus Infections/*complications/virology
MH  - Dermis/metabolism/pathology/virology
MH  - Fibroblasts/metabolism/*pathology/virology
MH  - Fibrosis/metabolism/*pathology/virology
MH  - Herpesviridae Infections/*complications/virology
MH  - Herpesvirus 6, Human/isolation & purification
MH  - Humans
MH  - Scleroderma, Systemic/metabolism/*pathology/virology
PMC - PMC7504027
OTO - NOTNLM
OT  - apoptosis
OT  - fibrosis
OT  - human cytomegalovirus
OT  - human herpesvirus 6
OT  - systemic sclerosis
COIS- The authors declare no conflict of interest.
EDAT- 2020/09/10 06:00
MHDA- 2021/03/26 06:00
PMCR- 2020/09/01
CRDT- 2020/09/09 01:02
PHST- 2020/07/29 00:00 [received]
PHST- 2020/08/26 00:00 [revised]
PHST- 2020/08/31 00:00 [accepted]
PHST- 2020/09/09 01:02 [entrez]
PHST- 2020/09/10 06:00 [pubmed]
PHST- 2021/03/26 06:00 [medline]
PHST- 2020/09/01 00:00 [pmc-release]
AID - ijms21176397 [pii]
AID - ijms-21-06397 [pii]
AID - 10.3390/ijms21176397 [doi]
PST - epublish
SO  - Int J Mol Sci. 2020 Sep 3;21(17):6397. doi: 10.3390/ijms21176397.