PMID- 32899579
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201027
IS  - 2076-393X (Print)
IS  - 2076-393X (Electronic)
IS  - 2076-393X (Linking)
VI  - 8
IP  - 3
DP  - 2020 Sep 5
TI  - Porcine Reproductive and Respiratory Syndrome Virus Interferes with Swine 
      Influenza A Virus Infection of Epithelial Cells.
LID - 10.3390/vaccines8030508 [doi]
LID - 508
AB  - Respiratory infections are still a major concern in pigs. Amongst the involved 
      viruses, the porcine reproductive and respiratory syndrome virus (PRRSV) and the 
      swine influenza type A virus (swIAV) have a major impact. These viruses 
      frequently encounter and dual infections are reported. We analyzed here the 
      molecular interactions between viruses and porcine tracheal epithelial cells as 
      well as lung tissue. PRRSV-1 species do not infect porcine respiratory epithelial 
      cells. However, PRRSV-1, when inoculated simultaneously or shortly before swIAV, 
      was able to inhibit swIAV H1N2 infection, modulate the interferon response and 
      alter signaling protein phosphorylations (ERK, AKT, AMPK, and JAK2), in our 
      conditions. SwIAV inhibition was also observed, although at a lower level, by 
      inactivated PRRSV-1, whereas acid wash treatment inactivating non-penetrated 
      viruses suppressed the interference effect. PRRSV-1 and swIAV may interact at 
      several stages, before their attachment to the cells, when they attach to their 
      receptors, and later on. In conclusion, we showed for the first time that PRRSV 
      can alter the relation between swIAV and its main target cells, opening the doors 
      to further studies on the interplay between viruses. Consequences of these 
      peculiar interactions on viral infections and vaccinations using modified live 
      vaccines require further investigations.
FAU - Saade, Georges
AU  - Saade G
AUID- ORCID: 0000-0001-6504-9604
AD  - BIOEPAR, INRAE, Oniris, 44307 Nantes, France.
FAU - Menard, Deborah
AU  - Menard D
AD  - BIOEPAR, INRAE, Oniris, 44307 Nantes, France.
FAU - Hervet, Caroline
AU  - Hervet C
AUID- ORCID: 0000-0003-3249-292X
AD  - BIOEPAR, INRAE, Oniris, 44307 Nantes, France.
FAU - Renson, Patricia
AU  - Renson P
AUID- ORCID: 0000-0001-8329-8479
AD  - ANSES, Ploufragan-Plouzane-Niort Laboratory, Swine Virology Immunology Unit, 
      22440 Ploufragan, France.
FAU - Hue, Erika
AU  - Hue E
AUID- ORCID: 0000-0001-6930-3606
AD  - LABEO Frank Duncombe, 14280 Saint-Contest, France.
AD  - Normandy University, UNICAEN ImpedanCELL, Federative Structure 4206 ICORE, 14280 
      Saint-Contest, France.
FAU - Zhu, Jianzhong
AU  - Zhu J
AUID- ORCID: 0000-0002-7082-1993
AD  - College of Veterinary Medicine, Comparative Medicine Research Institute, Yangzhou 
      University, Yangzhou 225009, China.
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety, 
      Yangzhou 225009, China.
FAU - Dubreil, Laurence
AU  - Dubreil L
AD  - PAnTher, INRAE, Oniris, 44307 Nantes, France.
FAU - Paillot, Romain
AU  - Paillot R
AUID- ORCID: 0000-0003-3081-2037
AD  - LABEO Frank Duncombe, 14280 Saint-Contest, France.
AD  - Normandy University, UNICAEN ImpedanCELL, Federative Structure 4206 ICORE, 14280 
      Saint-Contest, France.
FAU - Pronost, Stephane
AU  - Pronost S
AD  - LABEO Frank Duncombe, 14280 Saint-Contest, France.
AD  - Normandy University, UNICAEN ImpedanCELL, Federative Structure 4206 ICORE, 14280 
      Saint-Contest, France.
FAU - Bourry, Olivier
AU  - Bourry O
AUID- ORCID: 0000-0003-0220-0922
AD  - ANSES, Ploufragan-Plouzane-Niort Laboratory, Swine Virology Immunology Unit, 
      22440 Ploufragan, France.
FAU - Simon, Gaelle
AU  - Simon G
AD  - ANSES, Ploufragan-Plouzane-Niort Laboratory, Swine Virology Immunology Unit, 
      22440 Ploufragan, France.
FAU - Dupont, Joelle
AU  - Dupont J
AD  - PRC, INRAE, 37380 Nouzilly, France.
FAU - Bertho, Nicolas
AU  - Bertho N
AD  - BIOEPAR, INRAE, Oniris, 44307 Nantes, France.
FAU - Meurens, Francois
AU  - Meurens F
AUID- ORCID: 0000-0002-0353-4871
AD  - BIOEPAR, INRAE, Oniris, 44307 Nantes, France.
LA  - eng
GR  - CENTAURE European project/Normandy County Council and European Union in the 
      framework of the ERDF-ESF operational programme 2014-2020/
GR  - RFI Food for tomorrow-Cap aliment - Establishment grant F. Meurens/Conseil 
      Regional des Pays de la Loire/
PT  - Journal Article
DEP - 20200905
PL  - Switzerland
TA  - Vaccines (Basel)
JT  - Vaccines
JID - 101629355
PMC - PMC7565700
OTO - NOTNLM
OT  - PRRSV
OT  - coinfection
OT  - epithelial cell
OT  - interference
OT  - pig
OT  - swIAV
COIS- The authors declare no conflict of interest.
EDAT- 2020/09/10 06:00
MHDA- 2020/09/10 06:01
PMCR- 2020/09/05
CRDT- 2020/09/09 01:04
PHST- 2020/08/13 00:00 [received]
PHST- 2020/09/01 00:00 [revised]
PHST- 2020/09/03 00:00 [accepted]
PHST- 2020/09/09 01:04 [entrez]
PHST- 2020/09/10 06:00 [pubmed]
PHST- 2020/09/10 06:01 [medline]
PHST- 2020/09/05 00:00 [pmc-release]
AID - vaccines8030508 [pii]
AID - vaccines-08-00508 [pii]
AID - 10.3390/vaccines8030508 [doi]
PST - epublish
SO  - Vaccines (Basel). 2020 Sep 5;8(3):508. doi: 10.3390/vaccines8030508.