PMID- 32901501
OWN - NLM
STAT- MEDLINE
DCOM- 20210908
LR  - 20220210
IS  - 1802-9973 (Electronic)
IS  - 0862-8408 (Print)
IS  - 0862-8408 (Linking)
VI  - 69
IP  - 5
DP  - 2020 Nov 16
TI  - Simulated microgravity reduces proliferation and reorganizes the cytoskeleton of 
      human umbilical cord mesenchymal stem cells.
PG  - 897-906
AB  - The cytoskeleton plays a key role in cellular proliferation, cell-shape 
      maintenance and internal cellular organization. Cells are highly sensitive to 
      changes in microgravity, which can induce alterations in the distribution of the 
      cytoskeletal and cell proliferation. This study aimed to assess the effects of 
      simulated microgravity (SMG) on the proliferation and expression of major cell 
      cycle-related regulators and cytoskeletal proteins in human umbilical cord 
      mesenchymal stem cells (hucMSCs). A WST-1 assay showed that the proliferation of 
      SMG-exposed hucMSCs was lower than a control group. Furthermore, flow cytometry 
      analysis demonstrated that the percentage of SMG-exposed hucMSCs in the G0/G1 
      phase was higher than the control group. A western blot analysis revealed there 
      was a downregulation of cyclin A1 and A2 expression in SMG-exposed hucMSCs as 
      well. The expression of cyclin-dependent kinase 4 (cdk4) and 6 (cdk6) were also 
      observed to be reduced in the SMG-exposed hucMSCs. The total nuclear intensity of 
      SMG-exposed hucMSCs was also lower than the control group. However, there were no 
      differences in the nuclear area or nuclear-shape value of hucMSCs from the SMG 
      and control groups. A western blot and quantitative RT-PCR analysis showed that 
      SMG-exposed hucMSCs experienced a downregulation of bata-actin and alpha-tubulin 
      compared to the control group. SMG generated the reorganization of microtubules 
      and microfilaments in hucMSCs. Our study supports the idea that the 
      downregulation of major cell cycle-related proteins and cytoskeletal proteins 
      results in the remodeling of the cytoskeleton and the proliferation of hucMSCs.
FAU - Quynh Chi, H N
AU  - Quynh Chi HN
AD  - Animal Biotechnology Department, Institute of Tropical Biology, Ho Chi Minh City, 
      Vietnam. longlt@itb.ac.vn.
FAU - Nghia Son, H
AU  - Nghia Son H
FAU - Chinh Chung, D
AU  - Chinh Chung D
FAU - Huan, L D
AU  - Huan LD
FAU - Hong Diem, T
AU  - Hong Diem T
FAU - Long, L T
AU  - Long LT
LA  - eng
PT  - Journal Article
DEP - 20200909
PL  - Czech Republic
TA  - Physiol Res
JT  - Physiological research
JID - 9112413
SB  - IM
MH  - Actin Cytoskeleton/metabolism
MH  - Cell Cycle/physiology
MH  - Cell Differentiation/physiology
MH  - Cell Proliferation/physiology
MH  - Cells, Cultured
MH  - Cytoskeleton/*metabolism
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology/metabolism
MH  - Microtubules/metabolism
MH  - Umbilical Cord/*cytology/metabolism
MH  - *Weightlessness Simulation
PMC - PMC8549910
COIS- Conflict of Interest There is no conflict of interest.
EDAT- 2020/09/10 06:00
MHDA- 2021/09/09 06:00
PMCR- 2020/09/09
CRDT- 2020/09/09 08:46
PHST- 2020/09/10 06:00 [pubmed]
PHST- 2021/09/09 06:00 [medline]
PHST- 2020/09/09 08:46 [entrez]
PHST- 2020/09/09 00:00 [pmc-release]
AID - 934472 [pii]
AID - pr69_897 [pii]
AID - 10.33549/physiolres.934472 [doi]
PST - ppublish
SO  - Physiol Res. 2020 Nov 16;69(5):897-906. doi: 10.33549/physiolres.934472. Epub 
      2020 Sep 9.