PMID- 32901885
OWN - NLM
STAT- MEDLINE
DCOM- 20210426
LR  - 20210426
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 22
IP  - 5
DP  - 2020 Nov
TI  - Ghrelin system is involved in improvements in glucose metabolism mediated by 
      hyperbaric oxygen treatment in a streptozotocin‑induced type 1 diabetes mouse 
      model.
PG  - 3767-3776
LID - 10.3892/mmr.2020.11481 [doi]
AB  - Type 1 diabetes mellitus (T1DM) is an autoimmune disorder for which the only 
      effective therapy is insulin replacement. Hyperbaric oxygen (HBO) therapy has 
      demonstrated potential in improving hyperglycemia and as a treatment option for 
      T1DM. Ghrelin and HBO have been previously reported to exert proliferative, 
      anti‑apoptotic and anti‑inflammatory effects in pancreatic cells. The present 
      study investigated the mechanism underlying HBO‑ and ghrelin system‑mediated 
      regulation of glucose metabolism. Male C57BL/6 mice were intraperitoneally 
      injected with streptozotocin (STZ; 150 mg/kg) to induce T1DM before the diabetic 
      mice were randomly assigned into the T1DM and T1DM + HBO groups. Mice in the T1DM 
      + HBO group received HBO (1 h; 100% oxygen; 2 atmospheres absolute) daily for 
      2 weeks. Significantly lower blood glucose levels and food intake were observed 
      in mice in the T1DM + HBO group. Following HBO treatment, islet beta‑cell area were 
      increased whereas those of alpha‑cell were decreased in the pancreas. In addition, 
      greater hepatic glycogen storage in liver was observed, which coincided with 
      higher pancreatic glucose transporter 2 (GLUT2) expression levels and reduced 
      hepatic GLUT2 membrane trafficking. There were also substantially higher total 
      plasma ghrelin concentrations and gastric ghrelin‑O‑acyl transferase (GOAT) 
      expression levels in mice in the T1DM + HBO group. HBO treatment also abolished 
      reductions in pancreatic GOAT expression levels in T1DM mice. Additionally, 
      hepatic growth hormone secretagogue receptor‑1a levels were found to be lower in 
      mice in the T1DM + HBO group compared with those in the T1DM group. These results 
      suggest that HBO administration improved glucose metabolism in a STZ‑induced T1DM 
      mouse model. The underlying mechanism involves improved insulin‑release, 
      glucose‑sensing and regulation of hepatic glycogen storage, an observation that 
      was also likely dependent on the ghrelin signalling system.
FAU - Song, Limin
AU  - Song L
AD  - Department of Special Medicine, Medical College, Qingdao University, Qingdao, 
      Shandong 266071, P.R. China.
FAU - Yuan, Junhua
AU  - Yuan J
AD  - Department of Special Medicine, Medical College, Qingdao University, Qingdao, 
      Shandong 266071, P.R. China.
FAU - Liu, Yuan
AU  - Liu Y
AD  - Department of Special Medicine, Medical College, Qingdao University, Qingdao, 
      Shandong 266071, P.R. China.
FAU - Zhang, Di
AU  - Zhang D
AD  - Department of Special Medicine, Medical College, Qingdao University, Qingdao, 
      Shandong 266071, P.R. China.
FAU - Zhang, Caishun
AU  - Zhang C
AD  - Department of Special Medicine, Medical College, Qingdao University, Qingdao, 
      Shandong 266071, P.R. China.
FAU - Lin, Qian
AU  - Lin Q
AD  - Department of Special Medicine, Medical College, Qingdao University, Qingdao, 
      Shandong 266071, P.R. China.
FAU - Li, Manwen
AU  - Li M
AD  - Department of Special Medicine, Medical College, Qingdao University, Qingdao, 
      Shandong 266071, P.R. China.
FAU - Su, Kaizhen
AU  - Su K
AD  - Department of Clinical Medicine, Medical College, Qingdao University, Qingdao, 
      Shandong 266071, P.R. China.
FAU - Li, Yanrun
AU  - Li Y
AD  - Department of Clinical Medicine, Medical College, Qingdao University, Qingdao, 
      Shandong 266071, P.R. China.
FAU - Gao, Guangkai
AU  - Gao G
AD  - Department of Hyperbaric Medicine, Hospital of Chinese People's Liberation Army, 
      Qingdao, Shandong 266072, P.R. China.
FAU - Ma, Ruixia
AU  - Ma R
AD  - Department of Nephrology, Affiliated Hospital of Qingdao University, Qingdao, 
      Shandong 266005, P.R. China.
FAU - Dong, Jing
AU  - Dong J
AD  - Department of Special Medicine, Medical College, Qingdao University, Qingdao, 
      Shandong 266071, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20200902
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Blood Glucose)
RN  - 0 (Ghrelin)
RN  - 0 (Glucose Transporter Type 2)
RN  - 0 (Slc2a2 protein, mouse)
RN  - 5W494URQ81 (Streptozocin)
SB  - IM
MH  - Animals
MH  - Blood Glucose/drug effects
MH  - Diabetes Mellitus, Experimental/metabolism/*therapy
MH  - Diabetes Mellitus, Type 1/chemically induced/metabolism/*therapy
MH  - Ghrelin/*metabolism
MH  - Glucose Transporter Type 2/metabolism
MH  - Hyperbaric Oxygenation/*methods
MH  - Islets of Langerhans/drug effects/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Streptozocin
MH  - Treatment Outcome
PMC - PMC7533472
EDAT- 2020/09/10 06:00
MHDA- 2021/04/27 06:00
PMCR- 2020/09/02
CRDT- 2020/09/09 08:47
PHST- 2020/04/23 00:00 [received]
PHST- 2020/07/28 00:00 [accepted]
PHST- 2020/09/10 06:00 [pubmed]
PHST- 2021/04/27 06:00 [medline]
PHST- 2020/09/09 08:47 [entrez]
PHST- 2020/09/02 00:00 [pmc-release]
AID - mmr-22-05-3767 [pii]
AID - 10.3892/mmr.2020.11481 [doi]
PST - ppublish
SO  - Mol Med Rep. 2020 Nov;22(5):3767-3776. doi: 10.3892/mmr.2020.11481. Epub 2020 Sep 
      2.