PMID- 32901999
OWN - NLM
STAT- MEDLINE
DCOM- 20210430
LR  - 20210430
IS  - 1530-6860 (Electronic)
IS  - 0892-6638 (Linking)
VI  - 34
IP  - 11
DP  - 2020 Nov
TI  - Restriction of chronic Escherichia coli urinary tract infection depends upon T 
      cell-derived interleukin-17, a deficiency of which predisposes to flagella-driven 
      bacterial persistence.
PG  - 14572-14587
LID - 10.1096/fj.202000760R [doi]
AB  - Urinary tract infections (UTI) frequently progress to chronicity in infected 
      individuals but the mechanisms of pathogenesis underlying chronic UTI are not 
      well understood. We examined the role of interleukin (IL)-17A in UTI because this 
      cytokine promotes innate defense against uropathogenic Escherichia coli (UPEC). 
      Analysis of UPEC persistence and pyelonephritis in mice deficient in IL-17A 
      revealed that UPEC CFT073 caused infection at a rate higher than the multidrug 
      resistant strain EC958. Il17a(-/-) mice exhibited pyelonephritis with kidney 
      bacterial burdens higher than those of wild-type (WT) mice. Synthesis of IL-17A 
      in the bladder reflected a combination of gammadelta-T and T(H) 17 cell responses. 
      Analysis of circulating inflammatory mediators at 24h postinoculation identified 
      predictors of progression to chronicity, including IL-6 and monocyte 
      chemoattractant protein-1 (MCP-1). Histological analysis identified infiltrating 
      populations of neutrophils, NK cells, and gammadelta T cells in the bladder, whereas 
      neutrophils predominated in the kidney. Analysis of the contribution of flagella 
      to chronicity using hyper-flagellated and fliC-deficient UPEC in WT and 
      Il17a(-/-) mice revealed that, in a host that is deficient for the production of 
      IL-17A, flagella contribute to bacterial persistence. These findings show a role 
      for IL-17A in defense against chronic UTI and a contribution of flagella to the 
      pathogenesis of infection.
CI  - (c) 2020 Federation of American Societies for Experimental Biology.
FAU - Chamoun, Michelle N
AU  - Chamoun MN
AD  - School of Medical Sciences, And Menzies Health Institute Queensland, Griffith 
      University, Parklands, QLD, Australia.
FAU - Sullivan, Matthew J
AU  - Sullivan MJ
AD  - School of Medical Sciences, And Menzies Health Institute Queensland, Griffith 
      University, Parklands, QLD, Australia.
FAU - Goh, Kelvin G K
AU  - Goh KGK
AD  - School of Medical Sciences, And Menzies Health Institute Queensland, Griffith 
      University, Parklands, QLD, Australia.
FAU - Acharya, Dhruba
AU  - Acharya D
AD  - School of Medical Sciences, And Menzies Health Institute Queensland, Griffith 
      University, Parklands, QLD, Australia.
FAU - Ipe, Deepak S
AU  - Ipe DS
AD  - School of Medical Sciences, And Menzies Health Institute Queensland, Griffith 
      University, Parklands, QLD, Australia.
FAU - Katupitiya, Lahiru
AU  - Katupitiya L
AD  - School of Medical Sciences, And Menzies Health Institute Queensland, Griffith 
      University, Parklands, QLD, Australia.
FAU - Gosling, Dean
AU  - Gosling D
AD  - School of Medical Sciences, And Menzies Health Institute Queensland, Griffith 
      University, Parklands, QLD, Australia.
FAU - Peters, Kate M
AU  - Peters KM
AD  - School of Chemistry and Molecular Biosciences, Australian Infectious Diseases 
      Research Centre, University of Queensland, Brisbane, QLD, Australia.
FAU - Sweet, Matthew J
AU  - Sweet MJ
AD  - Institute for Molecular Bioscience (IMB), IMB Centre for Inflammation and Disease 
      Research, Australian Infectious Diseases Research Centre, The University of 
      Queensland, Brisbane, QLD, Australia.
FAU - Sester, David P
AU  - Sester DP
AD  - TRI Flow Cytometry Suite (TRI.fcs), Translational Research Institute, 
      Wooloongabba, QLD, Australia.
FAU - Schembri, Mark A
AU  - Schembri MA
AD  - School of Chemistry and Molecular Biosciences, Australian Infectious Diseases 
      Research Centre, University of Queensland, Brisbane, QLD, Australia.
FAU - Ulett, Glen C
AU  - Ulett GC
AD  - School of Medical Sciences, And Menzies Health Institute Queensland, Griffith 
      University, Parklands, QLD, Australia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200909
PL  - United States
TA  - FASEB J
JT  - FASEB journal : official publication of the Federation of American Societies for 
      Experimental Biology
JID - 8804484
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (FliC protein, E coli)
RN  - 0 (Il17a protein, mouse)
RN  - 0 (Interleukin-17)
RN  - 12777-81-0 (Flagellin)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2/metabolism
MH  - Escherichia coli Proteins/genetics/metabolism
MH  - Female
MH  - Flagella/genetics/*metabolism
MH  - Flagellin/genetics/metabolism
MH  - Host-Pathogen Interactions
MH  - *Immunity, Innate
MH  - Interleukin-17/genetics/*metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - T-Lymphocyte Subsets/*immunology
MH  - Urinary Bladder/cytology/immunology/microbiology
MH  - Urinary Tract Infections/genetics/*immunology/microbiology
MH  - Uropathogenic Escherichia coli/genetics/*pathogenicity/physiology
OTO - NOTNLM
OT  - Escherichia coli
OT  - Gram-negative pathogens
OT  - bacterial pathogenesis
OT  - innate immunity
OT  - urinary tract infection
EDAT- 2020/09/10 06:00
MHDA- 2021/05/01 06:00
CRDT- 2020/09/09 08:49
PHST- 2020/03/31 00:00 [received]
PHST- 2020/07/30 00:00 [revised]
PHST- 2020/08/18 00:00 [accepted]
PHST- 2020/09/10 06:00 [pubmed]
PHST- 2021/05/01 06:00 [medline]
PHST- 2020/09/09 08:49 [entrez]
AID - 10.1096/fj.202000760R [doi]
PST - ppublish
SO  - FASEB J. 2020 Nov;34(11):14572-14587. doi: 10.1096/fj.202000760R. Epub 2020 Sep 
      9.