PMID- 32902370
OWN - NLM
STAT- MEDLINE
DCOM- 20210609
LR  - 20210609
IS  - 2222-1751 (Electronic)
IS  - 2222-1751 (Linking)
VI  - 9
IP  - 1
DP  - 2020 Dec
TI  - mRNA and miRNA profiling of Zika virus-infected human umbilical cord mesenchymal 
      stem cells identifies miR-142-5p as an antiviral factor.
PG  - 2061-2075
LID - 10.1080/22221751.2020.1821581 [doi]
AB  - Zika virus (ZIKV) infection during pregnancy is associated with congenital brain 
      abnormalities, a finding that highlights the urgent need to understand 
      mother-to-fetus transmission mechanisms. Human umbilical cord mesenchymal stem 
      cells (hUCMSCs) are susceptible to ZIKV infection but the underlying mechanisms 
      of viral susceptibility remain largely unexplored. In this study, we have 
      characterized and compared host mRNA and miRNA expression profiles in hUCMSCs 
      after infection with two lineages of ZIKV, African (MR766) and Asian (PRVABC59). 
      RNA sequencing analysis identified differentially expressed genes involved in 
      anti-viral immunity and mitochondrial dynamics following ZIKV infection. In 
      particular, ZIKV-infected hUCMSCs displayed mitochondrial elongation and the 
      treatment of hUCMSCs with mitochondrial fission inhibitor led to a dose-dependent 
      increase in ZIKV gene expression and decrease in anti-viral signalling pathways. 
      Moreover, small RNA sequencing analysis identified several significantly up- or 
      down-regulated microRNAs. Interestingly, miR-142-5p was significantly 
      downregulated upon ZIKV infection, whereas cellular targets of miR-142-5p, IL6ST 
      and ITGAV, were upregulated. Overexpression of miR-142-5p resulted in the 
      suppression of ZIKV replication. Furthermore, blocking ITGAV expression resulted 
      in a significant suppression of ZIKV binding to cells, suggesting a potential 
      role of ITGAV in ZIKV entry. In conclusion, these results demonstrate both common 
      and specific host responses to African and Asian ZIKV lineages and indicate 
      miR-142-5p as a key regulator of ZIKV replication in the umbilical cords.
FAU - Seong, Rak-Kyun
AU  - Seong RK
AD  - Department of Biomedical Sciences, College of Medicine, Korea University Guro 
      Hospital, Seoul, Republic of Korea.
FAU - Lee, Jae Kyung
AU  - Lee JK
AD  - Department of Biomedical Sciences, College of Medicine, Korea University Guro 
      Hospital, Seoul, Republic of Korea.
FAU - Cho, Geum Joon
AU  - Cho GJ
AD  - Department of Obstetrics and Gynaecology, College of Medicine, Korea University 
      Guro Hospital, Seoul, Republic of Korea.
FAU - Kumar, Mukesh
AU  - Kumar M
AUID- ORCID: 0000-0003-0970-4875
AD  - Department of Biology, Georgia State University, Atlanta, Georgia, USA.
FAU - Shin, Ok Sarah
AU  - Shin OS
AD  - Department of Biomedical Sciences, College of Medicine, Korea University Guro 
      Hospital, Seoul, Republic of Korea.
LA  - eng
GR  - R21 NS099838/NS/NINDS NIH HHS/United States
GR  - R21 OD024896/OD/NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - Emerg Microbes Infect
JT  - Emerging microbes & infections
JID - 101594885
RN  - 0 (Antiviral Agents)
RN  - 0 (MIRN142 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - A549 Cells
MH  - Animals
MH  - Antiviral Agents/immunology
MH  - Cell Line
MH  - Cells, Cultured
MH  - Chlorocebus aethiops
MH  - *Gene Expression Profiling
MH  - Gene Expression Regulation
MH  - HeLa Cells
MH  - Host Microbial Interactions
MH  - Humans
MH  - Immunity, Innate
MH  - Mesenchymal Stem Cells/virology
MH  - MicroRNAs/*genetics
MH  - Mitochondria/*metabolism/virology
MH  - RNA, Messenger/*genetics
MH  - Sequence Analysis, RNA
MH  - Signal Transduction
MH  - Umbilical Cord/virology
MH  - Vero Cells
MH  - Virus Attachment
MH  - Virus Internalization
MH  - Virus Replication
MH  - Zika Virus
MH  - Zika Virus Infection/*genetics/immunology
PMC - PMC7534337
OTO - NOTNLM
OT  - RNA-seq
OT  - ZIKV
OT  - hUCMSCs
OT  - innate immunity
OT  - miRNA
OT  - small RNA-seq
COIS- No potential conflict of interest was reported by the author(s).
EDAT- 2020/09/10 06:00
MHDA- 2021/06/10 06:00
PMCR- 2020/09/22
CRDT- 2020/09/09 12:23
PHST- 2020/09/10 06:00 [pubmed]
PHST- 2021/06/10 06:00 [medline]
PHST- 2020/09/09 12:23 [entrez]
PHST- 2020/09/22 00:00 [pmc-release]
AID - 1821581 [pii]
AID - 10.1080/22221751.2020.1821581 [doi]
PST - ppublish
SO  - Emerg Microbes Infect. 2020 Dec;9(1):2061-2075. doi: 
      10.1080/22221751.2020.1821581.