PMID- 32902972
OWN - NLM
STAT- MEDLINE
DCOM- 20210106
LR  - 20220531
IS  - 1520-5851 (Electronic)
IS  - 0013-936X (Linking)
VI  - 54
IP  - 19
DP  - 2020 Oct 6
TI  - Nontarget Screening and Toxicity Evaluation of Diol Esters of Okadaic Acid and 
      Dinophysistoxins Reveal Intraspecies Difference of Prorocentrum lima.
PG  - 12366-12375
LID - 10.1021/acs.est.0c03691 [doi]
AB  - High-resolution mass spectrometry (HRMS) analysis with the assistance of 
      molecular networking was used to investigate intracellular toxin profiles of five 
      Prorocentrum lima (P. lima) strains sampled from the north Yellow Sea and South 
      China Sea. Mice were used as a model species for testing the acute toxicity of 
      intracellular okadaic acid (OA) and dinophysistoxins (DTXs) in free and 
      esterified states. Results showed that OA and DTX1 esterified derivatives were 
      detected in all P. lima samples, accounting for 55%-96% of total toxins in five 
      strains. A total of 24 esters and 1 stereoisomer of DTX1 (35S DTX1) were 
      identified based on molecular networking and MS data analysis, 15 esters of which 
      have been reported first. All P. lima strains displayed specific toxin profiles, 
      and preliminary analysis suggested that toxin profiles of the five P. lima 
      strains might be region-related. Moreover, acute toxicity in mice suggested 
      higher toxicity of esters compared with free toxins, which highlights the 
      importance and urgency of attention to esterified toxins in P. lima.
FAU - Wu, Haiyan
AU  - Wu H
AUID- ORCID: 0000-0001-5880-3998
AD  - Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, 
      Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research 
      Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
FAU - Chen, Jiaqi
AU  - Chen J
AUID- ORCID: 0000-0002-0925-8492
AD  - Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, 
      Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research 
      Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
FAU - Peng, Jixing
AU  - Peng J
AD  - Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, 
      Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research 
      Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
FAU - Zhong, Yun
AU  - Zhong Y
AD  - Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, 
      Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research 
      Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
FAU - Zheng, Guanchao
AU  - Zheng G
AD  - Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, 
      Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research 
      Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
FAU - Guo, Mengmeng
AU  - Guo M
AD  - Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, 
      Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research 
      Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
FAU - Tan, Zhijun
AU  - Tan Z
AUID- ORCID: 0000-0002-7216-4034
AD  - Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, 
      Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research 
      Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
AD  - Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 
      266071, China.
FAU - Zhai, Yuxiu
AU  - Zhai Y
AD  - Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, 
      Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research 
      Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
AD  - Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 
      266071, China.
FAU - Lu, Songhui
AU  - Lu S
AD  - College of Life Science and Technology, Jinan University, Guangzhou 510632, 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200920
PL  - United States
TA  - Environ Sci Technol
JT  - Environmental science & technology
JID - 0213155
RN  - 0 (Esters)
RN  - 0 (Marine Toxins)
RN  - 1W21G5Q4N2 (Okadaic Acid)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - Animals
MH  - China
MH  - *Dinoflagellida
MH  - Esters
MH  - *Marine Toxins/toxicity
MH  - Mice
MH  - Okadaic Acid/toxicity
MH  - Ubiquitin-Protein Ligases
EDAT- 2020/09/10 06:00
MHDA- 2021/01/07 06:00
CRDT- 2020/09/09 17:45
PHST- 2020/09/10 06:00 [pubmed]
PHST- 2021/01/07 06:00 [medline]
PHST- 2020/09/09 17:45 [entrez]
AID - 10.1021/acs.est.0c03691 [doi]
PST - ppublish
SO  - Environ Sci Technol. 2020 Oct 6;54(19):12366-12375. doi: 10.1021/acs.est.0c03691. 
      Epub 2020 Sep 20.