PMID- 32903481
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1664-042X (Print)
IS  - 1664-042X (Electronic)
IS  - 1664-042X (Linking)
VI  - 11
DP  - 2020
TI  - Effects of Human Umbilical Cord-Derived Mesenchymal Stem Cells on the Acute 
      Cigarette Smoke-Induced Pulmonary Inflammation Model.
PG  - 962
LID - 10.3389/fphys.2020.00962 [doi]
LID - 962
AB  - Cigarette smoke (CS) has been reported to induce oxidative stress and 
      inflammatory process in the lungs. However, the role of human umbilical 
      cord-derived mesenchymal stem cells (hUC-MSCs) in the regulation of pulmonary 
      inflammation remains unclear. The objective of this study is to investigate the 
      effects of hUC-MSCs on lung inflammation in the acute CS-induced pulmonary 
      inflammation animal model. Eight-week-old male C57BL/6 mice were intravenously 
      administered 3 x 10(6), 1 x 10(7), and 3 x 10(7) cells/kg of hUC-MSCs as well as 
      normal saline alone (control) after 3 days of CS exposure. Mice exposed to 
      high-efficiency particulate air (HEPA)-filtered room air served as the CS control 
      group. High-dose (3 x 10(7) cells/kg) hUC-MSC administration significantly 
      decreased tumor necrosis factor (TNF)-alpha in the bronchoalveolar lavage fluid 
      (BALF) of CS-exposed mice (p < 0.05). The chemokine (CXC motif) ligand 
      1/keratinocyte chemoattractant (CXCL1/KC) in BALF were significantly reduced by 
      low-dose (3 x 10(6) cells/kg) and high-dose (3 x 10(7) cells/kg) hUC-MSC (p < 
      0.05). Medium-dose hUC-MSC administration decreased interleukin (IL)-1beta in lung 
      of mice, and TNF-alpha and caspase-3 were decreased in the lung of CS-exposed mice by 
      medium- and high-dose MSC (p < 0.05). Low-dose hUC-MSCs significantly elevated 
      serum CXCL1/KC and IL-1beta in CS-exposed mice (p < 0.05). Our results suggest that 
      high-dose hUC-MSCs reduced pulmonary inflammation and had antiapoptotic effects 
      in acute pulmonary inflammation.
CI  - Copyright (c) 2020 Chen, Chen, Lin, Chien, Chen, Wen, Hsiao and Chuang.
FAU - Chen, Xiao-Yue
AU  - Chen XY
AD  - School of Respiratory Therapy, College of Medicine, Taipei Medical University, 
      Taipei, Taiwan.
FAU - Chen, Yi-Ying
AU  - Chen YY
AD  - School of Respiratory Therapy, College of Medicine, Taipei Medical University, 
      Taipei, Taiwan.
FAU - Lin, Willie
AU  - Lin W
AD  - Meridigen Biotech Co. Ltd., Taipei, Taiwan.
FAU - Chien, Chia-Wen
AU  - Chien CW
AD  - Meridigen Biotech Co. Ltd., Taipei, Taiwan.
FAU - Chen, Chien-Han
AU  - Chen CH
AD  - Meridigen Biotech Co. Ltd., Taipei, Taiwan.
FAU - Wen, Yu-Chieh
AU  - Wen YC
AD  - Meridigen Biotech Co. Ltd., Taipei, Taiwan.
FAU - Hsiao, Ta-Chih
AU  - Hsiao TC
AD  - Graduate Institute of Environmental Engineering, National Taiwan University, 
      Taipei, Taiwan.
FAU - Chuang, Hsiao-Chi
AU  - Chuang HC
AD  - School of Respiratory Therapy, College of Medicine, Taipei Medical University, 
      Taipei, Taiwan.
AD  - Cell Physiology and Molecular Image Research Center, Wan Fang Hospital, Taipei 
      Medical University, Taipei, Taiwan.
AD  - Division of Pulmonary Medicine, Department of Internal Medicine, Shuang Ho 
      Hospital, Taipei Medical University, New Taipei City, Taiwan.
LA  - eng
PT  - Journal Article
DEP - 20200812
PL  - Switzerland
TA  - Front Physiol
JT  - Frontiers in physiology
JID - 101549006
PMC - PMC7434987
OTO - NOTNLM
OT  - cigarette smoke
OT  - inflammation
OT  - lung
OT  - mesenchymal stem cells
OT  - oxidative stress
EDAT- 2020/09/10 06:00
MHDA- 2020/09/10 06:01
PMCR- 2020/08/12
CRDT- 2020/09/09 17:54
PHST- 2020/05/14 00:00 [received]
PHST- 2020/07/15 00:00 [accepted]
PHST- 2020/09/09 17:54 [entrez]
PHST- 2020/09/10 06:00 [pubmed]
PHST- 2020/09/10 06:01 [medline]
PHST- 2020/08/12 00:00 [pmc-release]
AID - 10.3389/fphys.2020.00962 [doi]
PST - epublish
SO  - Front Physiol. 2020 Aug 12;11:962. doi: 10.3389/fphys.2020.00962. eCollection 
      2020.