PMID- 32904666
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220416
IS  - 1178-6930 (Print)
IS  - 1178-6930 (Electronic)
IS  - 1178-6930 (Linking)
VI  - 13
DP  - 2020
TI  - Exosomal-lncRNA DLEU1 Accelerates the Proliferation, Migration, and Invasion of 
      Endometrial Carcinoma Cells by Regulating microRNA-E2F3.
PG  - 8651-8663
LID - 10.2147/OTT.S262661 [doi]
AB  - PURPOSE: Long non-coding RNAs (lncRNAs) may act as oncogenes in several cancers, 
      including endometrial carcinoma (EC). The purpose of the current study is to 
      investigate the regulatory mechanism of exosomal-lncRNA deleted in lymphocytic 
      leukemia1 (DLEU1) on EC. METHODS: The expression levels of lncRNA DLEU1, 
      microRNA-381-3p and E2F Transcription Factor 3 (E2F3) in EC tissues or cells were 
      detected using quantitative reverse transcription-polymerase chain reaction 
      (qRT-PCR). We then analysed the proliferation, migration, and invasion of EC 
      cells by performing the MTT assay, wound healing assay, and transwell invasion 
      assay, respectively. Identification of exosomes was detected using Western blot 
      assay. The uptake of exosomes was detected by a confocal microscope. The effects 
      of exosomes on EC cells were investigated by construction of cell co-culture 
      system. The interactions among DLEU1, miR-381-3p and E2F3 were confirmed using 
      the dual-luciferase reporter (DLR) assay. RESULTS: LncRNA DLEU1 expression was 
      highly up-regulated in EC tissues and cells. Knockdown of DLEU1 inhibited the 
      proliferation, migration, and invasion of EC cells. Exosomes could be uptaken by 
      the ambient EC cells. MiR-381-3p was a target of DLEU1 and was negatively 
      modulated by DLEU1. Overexpression of miR-381-3p suppressed the proliferation, 
      migration, and invasion of EC cells. Additionally, E2F3 was the target gene of 
      miR-381-3p and was negatively modulated by miR-381-3p. Upregulation of miR-381-3p 
      and down-regulation of E2F3 reversed the promoting effect of exosomal DLEU1 on EC 
      cells. CONCLUSION: Exosomal DLEU1 accelerates the development of EC by regulating 
      the miR-381-3p/E2F3 axis, thus DLEU1 may act as a possible therapeutic target for 
      treating EC.
CI  - (c) 2020 Jia et al.
FAU - Jia, Jianjun
AU  - Jia J
AD  - Department of Obstetrics and Gynecology, The First Affiliated Hospital, Jinan 
      University, Guangzhou City, Guangdong Province 510632, People's Republic of 
      China.
FAU - Guo, Suiqun
AU  - Guo S
AD  - Department of Obstetrics and Gynecology, The Third Affiliated Hospital, Southern 
      Medical University, Guangzhou City, Guangdong Province, People's Republic of 
      China.
FAU - Zhang, Dong
AU  - Zhang D
AD  - Department of Obstetrics and Gynecology, The First Affiliated Hospital, Jinan 
      University, Guangzhou City, Guangdong Province 510632, People's Republic of 
      China.
FAU - Tian, Xiaohui
AU  - Tian X
AD  - Department of Obstetrics and Gynecology, The Seventh Affiliated Hospital, Sun 
      Yat-Sen University, Shenzhen City, Guangdong Province, People's Republic of 
      China.
FAU - Xie, Xingmei
AU  - Xie X
AD  - Department of Obstetrics and Gynecology, The First Affiliated Hospital, Jinan 
      University, Guangzhou City, Guangdong Province 510632, People's Republic of 
      China.
LA  - eng
PT  - Journal Article
DEP - 20200825
PL  - New Zealand
TA  - Onco Targets Ther
JT  - OncoTargets and therapy
JID - 101514322
PMC - PMC7457553
OTO - NOTNLM
OT  - E2F3
OT  - endometrial carcinoma
OT  - exosomes
OT  - lncRNA DLEU1
OT  - miR-381-3p
COIS- The authors report no conflicts of interest for this work.
EDAT- 2020/09/10 06:00
MHDA- 2020/09/10 06:01
PMCR- 2020/08/25
CRDT- 2020/09/09 18:06
PHST- 2020/05/13 00:00 [received]
PHST- 2020/07/24 00:00 [accepted]
PHST- 2020/09/09 18:06 [entrez]
PHST- 2020/09/10 06:00 [pubmed]
PHST- 2020/09/10 06:01 [medline]
PHST- 2020/08/25 00:00 [pmc-release]
AID - 262661 [pii]
AID - 10.2147/OTT.S262661 [doi]
PST - epublish
SO  - Onco Targets Ther. 2020 Aug 25;13:8651-8663. doi: 10.2147/OTT.S262661. 
      eCollection 2020.