PMID- 32904847
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231104
IS  - 2347-3584 (Print)
IS  - 2347-3517 (Electronic)
IS  - 2347-3584 (Linking)
VI  - 31
IP  - 3
DP  - 2020 Sep
TI  - Newcastle disease virus suppress glycolysis pathway and induce breast cancer 
      cells death.
PG  - 341-348
LID - 10.1007/s13337-020-00612-z [doi]
AB  - Newcastle disease virus (NDV) can modulate cancer cell signaling pathway and 
      induce apoptosis in cancer cells. Cancer cells increase their glycolysis rates to 
      meet the energy demands for their survival and generate ATP as the primary energy 
      source for cell growth and proliferation. Interfering the glycolysis pathway may 
      be a valuable antitumor strategy. This study aimed to assess the effect of NDV on 
      the glycolysis pathway in infected breast cancer cells. Oncolytic NDV attenuated 
      AMHA1 strain was used in this study. AMJ13 and MCF7 breast cancer cell lines and 
      a normal embryonic REF cell line were infected with NDV with different 
      multiplicity of infections (moi) to determine the IC50 of NDV through MTT assay. 
      Crystal violet staining was done to study the morphological changes. NDV 
      apoptosis induction was assessed using AO/PI assay. NDV interference with the 
      glycolysis pathway was examined through measuring hexokinase (HK) activity, 
      pyruvate, and ATP concentrations, and pH levels in NDV infected and non-infected 
      breast cancer cells and in normal embryonic cells. The results showed that NDV 
      replicates efficiently in cancer cells and spare normal cells and induce 
      morphological changes and apoptosis in breast cancer cells but not in normal 
      cells. NDV infected cancer cells showed decreased in the HK activity, pyruvate 
      and ATP concentrations, and acidity, which reflect a significant decrease in the 
      glycolysis activity of the NDV infected tumor cells. No effects on the normal 
      cells were observed. In conclusion, oncolytic NDV ability to reduce glycolysis 
      pathway activity in cancer cells can be an exciting module to improve antitumor 
      therapeutics.
CI  - (c) Indian Virological Society 2020.
FAU - Al-Ziaydi, Ahmed Ghdhban
AU  - Al-Ziaydi AG
AD  - Department of Medical Chemistry, College of Medicine, University of Al-Qadisiyah, 
      Al Diwaniyah, Iraq. GRID: grid.440842.e. ISNI: 0000 0004 7474 9217
FAU - Al-Shammari, Ahmed Majeed
AU  - Al-Shammari AM
AUID- ORCID: 0000-0002-2699-1514
AD  - Department of Experimental Therapy, Iraqi Center of Cancer and Medical Genetics 
      Research, Mustansiriyah University, Baghdad, Iraq. GRID: grid.411309.e
FAU - Hamzah, Mohammed I
AU  - Hamzah MI
AD  - College of Medicine, University of Al-Nahrain, Baghdad, Iraq.
FAU - Kadhim, Haider Sabah
AU  - Kadhim HS
AD  - Department of Microbiology, College of Medicine, Al-Nahrain University, Baghdad, 
      Iraq. GRID: grid.411310.6. ISNI: 0000 0004 0636 1464
FAU - Jabir, Majid Sakhi
AU  - Jabir MS
AD  - Department of Applied Sciences, Technology University, Baghdad, Iraq.
LA  - eng
PT  - Journal Article
DEP - 20200627
PL  - India
TA  - Virusdisease
JT  - Virusdisease
JID - 101624144
PMC - PMC7458979
OTO - NOTNLM
OT  - Cancer metabolism
OT  - Oncolytics
OT  - Pyruvate
OT  - Virotherapy
OT  - Warburg effect
COIS- Conflict of interestThe authors disclose no potential conflict of interest.
EDAT- 2020/09/10 06:00
MHDA- 2020/09/10 06:01
PMCR- 2021/09/01
CRDT- 2020/09/09 18:08
PHST- 2020/01/17 00:00 [received]
PHST- 2020/06/18 00:00 [accepted]
PHST- 2020/09/09 18:08 [entrez]
PHST- 2020/09/10 06:00 [pubmed]
PHST- 2020/09/10 06:01 [medline]
PHST- 2021/09/01 00:00 [pmc-release]
AID - 612 [pii]
AID - 10.1007/s13337-020-00612-z [doi]
PST - ppublish
SO  - Virusdisease. 2020 Sep;31(3):341-348. doi: 10.1007/s13337-020-00612-z. Epub 2020 
      Jun 27.