PMID- 32907406
OWN - NLM
STAT- MEDLINE
DCOM- 20220408
LR  - 20220408
IS  - 1525-6014 (Electronic)
IS  - 0148-0545 (Linking)
VI  - 45
IP  - 3
DP  - 2022 May
TI  - Hepatotoxic effect of lead and hepatoprotective effect of Hydrilla verticillata 
      on hepatic transcriptional and physiological response in edible fish Labeo 
      rohita.
PG  - 1276-1283
LID - 10.1080/01480545.2020.1815762 [doi]
AB  - Lead tops the list of developmental toxicants released by industry into the 
      environment according to Commission for Environmental Corporation. Hydrilla 
      verticillata is an aquatic weed and rich source of variable nutrients and 
      chemical constituents like saponins, vitamins, minerals, antioxidants, amino 
      acids, detoxifying agents, etc. This weed is used in a beneficial way to detoxify 
      lead in fish model. The fish were treated with sublethal concentration of lead to 
      induce liver damage and fed with supplementary feed containing 20% of hydrilla 
      dry powder. The present work revealed that lead accumulation is highly toxic to 
      liver. Lead toxicity increased the expressions of cytochrome P450 1 A (CYP1A) and 
      cytochrome P450 3 A (CYP3A) when compared to control. The metal binding stress 
      proteins, heat shock proteins (Hsp60 & Hsp70) and metallothionein were also 
      upregulated due to lead toxicity. Lead intoxicated fish exhibited reduced 
      delta-aminolevulinic acid dehydratase activity in the blood, leading to reduced red 
      blood cell count and changes in cell morphology. The lead toxicity also decreases 
      the level of liver marker enzymes alanine transaminase, aspartate transaminase in 
      serum. In hydrilla supplemented protective group, there was amelioration of these 
      changes. Administration of supplementary feed to the lead intoxicated protective 
      group significantly decreased the expressions of CYP1A, CYP3A, Hsp60, Hsp70 and 
      metallothionein genes. The red blood cell count in lead intoxicated fish was 
      maintained almost normal due to the protective action of Hydrilla verticillata in 
      the diet. The observed increase in the enzymes, which further confirmed the 
      protective effect of Hydrilla verticillata against lead induced toxicity.
FAU - S, Pandi Prabha
AU  - S PP
AD  - Department of Biotechnology, Sri Venkateswara College of Engineering, Chennai, 
      India.
FAU - Rajkumar, Johanna
AU  - Rajkumar J
AD  - Department of Biotechnology, Rajalakshmi Engineering College, Chennai, India.
FAU - C, Karthik
AU  - C K
AD  - Department of Biotechnology, St. Joseph's College of Engineering, OMR, Chennai, 
      India.
LA  - eng
PT  - Journal Article
DEP - 20200909
PL  - United States
TA  - Drug Chem Toxicol
JT  - Drug and chemical toxicology
JID - 7801723
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 2P299V784P (Lead)
RN  - 9038-94-2 (Metallothionein)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP3A)
SB  - IM
MH  - Animals
MH  - *Cyprinidae
MH  - Cytochrome P-450 CYP3A/metabolism
MH  - HSP70 Heat-Shock Proteins/genetics/metabolism
MH  - *Hydrocharitaceae
MH  - Lead/metabolism/toxicity
MH  - Liver
MH  - Metallothionein/metabolism
OTO - NOTNLM
OT  - Alanine transaminase
OT  - Aspartate transaminase
OT  - Cytochrome P450
OT  - Red blood cell count
OT  - mRNA transcript
OT  - delta-Aminolevulinic acid dehydratase
EDAT- 2020/09/11 06:00
MHDA- 2022/04/09 06:00
CRDT- 2020/09/10 05:24
PHST- 2020/09/11 06:00 [pubmed]
PHST- 2022/04/09 06:00 [medline]
PHST- 2020/09/10 05:24 [entrez]
AID - 10.1080/01480545.2020.1815762 [doi]
PST - ppublish
SO  - Drug Chem Toxicol. 2022 May;45(3):1276-1283. doi: 10.1080/01480545.2020.1815762. 
      Epub 2020 Sep 9.