PMID- 32908557
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240329
IS  - 1741-427X (Print)
IS  - 1741-4288 (Electronic)
IS  - 1741-427X (Linking)
VI  - 2020
DP  - 2020
TI  - Network Pharmacology-Based Approach to Revealing Biological Mechanisms of 
      Qingkailing Injection against IschemicStroke: Focusing on Blood-Brain Barrier.
PG  - 2914579
LID - 10.1155/2020/2914579 [doi]
LID - 2914579
AB  - Ischemic stroke is the most common type of cerebrovascular accident worldwide. It 
      causes long-term disability and death. Qingkailing (QKL) injection is a 
      traditional Chinese patent medicine which has been clinically applied in the 
      treatment of ischemic stroke for nearly thirty years. In the present study, 
      network pharmacology combined with experimentation was used to elucidate the 
      mechanisms of QKL. ADME screening and target prediction identified 62 active 
      compounds and 275 targets for QKL. Topological screening of the protein-protein 
      interaction (PPI) network was used to build a core PPI network consisting of 408 
      nodes and 17,830 edges. KEGG enrichment indicated that the main signaling pathway 
      implicated in ischemic stroke involved hypoxia-inducible factor-1 (HIF-1). 
      Experimentation showed that QKL alleviated neurological deficits, brain 
      infraction, blood-brain barrier (BBB) leakage, and tight junction degeneration in 
      a mouse ischemic stroke model. Two-photon laser scanning microscopy was used to 
      evaluate BBB permeability and cerebral microvessel structure in living mice. 
      HIF-1alpha, matrix metalloproteinase-9 (MMP-9), and tight junction proteins such as 
      occludin, zonula occludins-1 (ZO-1), claudin-5, and VE-Cadherin were measured by 
      western blotting. QKL upregulated ZO-1 and downregulated HIF-1alpha and MMP-9. QKL 
      has a multiapproach, multitarget, and synergistic effect against ischemic stroke.
CI  - Copyright (c) 2020 Shuang Zhang et al.
FAU - Zhang, Shuang
AU  - Zhang S
AD  - Beijing Key Laboratory, School of Basic Medical Sciences, Beijing University of 
      Chinese Medicine, 11 Beisanhuandong Road, Chaoyang District, Beijing 100029, 
      China.
FAU - Wang, Xueqian
AU  - Wang X
AUID- ORCID: 0000-0001-9138-1491
AD  - Beijing Key Laboratory, School of Basic Medical Sciences, Beijing University of 
      Chinese Medicine, 11 Beisanhuandong Road, Chaoyang District, Beijing 100029, 
      China.
FAU - Cheng, Fafeng
AU  - Cheng F
AUID- ORCID: 0000-0002-3409-1196
AD  - Beijing Key Laboratory, School of Basic Medical Sciences, Beijing University of 
      Chinese Medicine, 11 Beisanhuandong Road, Chaoyang District, Beijing 100029, 
      China.
FAU - Ma, Chongyang
AU  - Ma C
AD  - Beijing Key Laboratory, School of Basic Medical Sciences, Beijing University of 
      Chinese Medicine, 11 Beisanhuandong Road, Chaoyang District, Beijing 100029, 
      China.
AD  - School of Traditional Chinese Medicine, Capital Medical University, Beijing 
      100069, China.
FAU - Fan, Shuning
AU  - Fan S
AD  - Beijing Key Laboratory, School of Basic Medical Sciences, Beijing University of 
      Chinese Medicine, 11 Beisanhuandong Road, Chaoyang District, Beijing 100029, 
      China.
FAU - Xu, Wenxiu
AU  - Xu W
AD  - Beijing Key Laboratory, School of Basic Medical Sciences, Beijing University of 
      Chinese Medicine, 11 Beisanhuandong Road, Chaoyang District, Beijing 100029, 
      China.
FAU - Jin, Na
AU  - Jin N
AD  - Beijing Key Laboratory, School of Basic Medical Sciences, Beijing University of 
      Chinese Medicine, 11 Beisanhuandong Road, Chaoyang District, Beijing 100029, 
      China.
FAU - Liu, Shuling
AU  - Liu S
AD  - Beijing Key Laboratory, School of Basic Medical Sciences, Beijing University of 
      Chinese Medicine, 11 Beisanhuandong Road, Chaoyang District, Beijing 100029, 
      China.
FAU - Lv, Kai
AU  - Lv K
AUID- ORCID: 0000-0002-8579-4342
AD  - The Third Affiliated Hospital of Beijing University of Chinese Medicine, 51 An 
      Wai Xiaoguan Street, Chaoyang District, Beijing 100029, China.
FAU - Wang, Qingguo
AU  - Wang Q
AUID- ORCID: 0000-0002-2865-903X
AD  - Beijing Key Laboratory, School of Basic Medical Sciences, Beijing University of 
      Chinese Medicine, 11 Beisanhuandong Road, Chaoyang District, Beijing 100029, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20200827
PL  - United States
TA  - Evid Based Complement Alternat Med
JT  - Evidence-based complementary and alternative medicine : eCAM
JID - 101215021
PMC - PMC7474352
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as potential 
      conflicts of interest.
EDAT- 2020/09/11 06:00
MHDA- 2020/09/11 06:01
PMCR- 2020/08/27
CRDT- 2020/09/10 05:35
PHST- 2020/03/02 00:00 [received]
PHST- 2020/06/18 00:00 [revised]
PHST- 2020/07/30 00:00 [accepted]
PHST- 2020/09/10 05:35 [entrez]
PHST- 2020/09/11 06:00 [pubmed]
PHST- 2020/09/11 06:01 [medline]
PHST- 2020/08/27 00:00 [pmc-release]
AID - 10.1155/2020/2914579 [doi]
PST - epublish
SO  - Evid Based Complement Alternat Med. 2020 Aug 27;2020:2914579. doi: 
      10.1155/2020/2914579. eCollection 2020.