PMID- 32909191 OWN - NLM STAT- MEDLINE DCOM- 20211123 LR - 20240226 IS - 1933-7205 (Electronic) IS - 1933-7191 (Linking) VI - 28 IP - 3 DP - 2021 Mar TI - TGF-beta1 Neutralization Improves Pregnancy Outcomes by Restoring Endometrial Receptivity in Mice with Adenomyosis. PG - 877-887 LID - 10.1007/s43032-020-00308-1 [doi] AB - The objective of this research is to study the effects of TGF-beta1 inhibition on endometrial receptivity and pregnancy outcomes in mice with adenomyosis. Experiments were done using a mouse model of adenomyosis which took place in a hospital-affiliated laboratory. The mouse model used for this research is ICR mouse. Adenomyosis was induced by oral gavage of tamoxifen (TAM) from postnatal days (PNDs) 1 to 4 in ICR mice. Bilateral intrauterine injection of anti-TGF-beta1-neutralizing antibody or isotype IgG or PBS was performed at PND42. The mice were then either sacrificed or mated at PND64 followed by sacrificing at gestational day (GD) 4 or proceeding to delivery. Implantation numbers, rate of dams with live birth, live birth numbers, survival at 1 week old, and pup mortality rate after weaning were recorded. Collagen was demonstrated by Masson's trichrome and Van Gieson's stains. Uterine expression of a receptivity marker, leukemia inhibitory factor (LIF), was examined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR), Western blot, and immunohistochemistry (IHC). Anti-TGF-beta1 treatment increased the mean implantation numbers, fecundity rate, the rate of dams with live birth, pup survival rate at 1 week old, and pup mortality rate after weaning. Collagen expression in uteri with adenomyosis was attenuated by anti-TGF-beta1 treatment. Increased LIF expression by anti-TGF-beta1 treatment was detected by qRT-PCR, Western blot, and IHC. The results suggest that inhibition of TGF-beta1 improves pregnancy outcomes by restoring endometrial receptivity in mice with adenomyosis. FAU - Kay, Nari AU - Kay N AD - Department of Obstetrics and Gynecology, E-Da Hospital, 6 Yida Rd., Jiaosu Village, Yanchao District, Kaohsiung, Taiwan. AD - Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, #100, Tzyou 1st Road, Kaohsiung, 807, Taiwan. AD - School of Medicine, College of Medicine, I-Shou University, Kaohsiung, Taiwan. FAU - Huang, Chun-Yen AU - Huang CY AD - Department of Medical Research, E-Da Hospital, Kaohsiung, Taiwan. FAU - Shiu, Li-Yen AU - Shiu LY AD - Department of Medical Research, E-Da Hospital, Kaohsiung, Taiwan. FAU - Yu, Ya-Chun AU - Yu YC AD - Department of Medical Research, E-Da Hospital, Kaohsiung, Taiwan. FAU - Chang, Yu AU - Chang Y AD - Department of Obstetrics and Gynecology, E-Da Hospital, 6 Yida Rd., Jiaosu Village, Yanchao District, Kaohsiung, Taiwan. AD - School of Medicine, College of Medicine, I-Shou University, Kaohsiung, Taiwan. FAU - Schatz, Frederick AU - Schatz F AD - Department of Obstetrics and Gynecology, Morsani College of Medicine, University of South Florida, 12901 Bruce B Downs Blvd., MDC48, Tampa, FL, 33612, USA. FAU - Suen, Jau-Ling AU - Suen JL AD - Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, #100, Tzyou 1st Road, Kaohsiung, 807, Taiwan. AD - Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan. FAU - Tsai, Eing-Mei AU - Tsai EM AD - Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, #100, Tzyou 1st Road, Kaohsiung, 807, Taiwan. tsaieing@yahoo.com. AD - Department of Obstetrics and Gynecology, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan. tsaieing@yahoo.com. FAU - Huang, S Joseph AU - Huang SJ AUID- ORCID: 0000-0003-4987-4756 AD - Department of Obstetrics and Gynecology, E-Da Hospital, 6 Yida Rd., Jiaosu Village, Yanchao District, Kaohsiung, Taiwan. jhuang3@usf.edu. AD - School of Medicine, College of Medicine, I-Shou University, Kaohsiung, Taiwan. jhuang3@usf.edu. AD - Department of Obstetrics and Gynecology, Morsani College of Medicine, University of South Florida, 12901 Bruce B Downs Blvd., MDC48, Tampa, FL, 33612, USA. jhuang3@usf.edu. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20200909 PL - United States TA - Reprod Sci JT - Reproductive sciences (Thousand Oaks, Calif.) JID - 101291249 RN - 0 (Antibodies, Neutralizing) RN - 0 (Leukemia Inhibitory Factor) RN - 0 (Lif protein, mouse) RN - 0 (Tgfb1 protein, mouse) RN - 0 (Transforming Growth Factor beta1) RN - 9007-34-5 (Collagen) SB - IM MH - Adenomyosis/complications/*drug therapy/metabolism/physiopathology MH - Animals MH - Antibodies, Neutralizing/*pharmacology MH - Collagen/metabolism MH - Disease Models, Animal MH - Embryo Implantation/*drug effects MH - Endometrium/*drug effects/metabolism/physiopathology MH - Female MH - Infertility, Female/etiology/metabolism/physiopathology/*prevention & control MH - Leukemia Inhibitory Factor/genetics/metabolism MH - Mice, Inbred ICR MH - Pregnancy MH - Transforming Growth Factor beta1/*antagonists & inhibitors/metabolism MH - Mice OTO - NOTNLM OT - Adenomyosis OT - Endometrial receptivity OT - Leukemia inhibitory factor OT - Tamoxifen OT - Transforming growth factor-beta1 EDAT- 2020/09/11 06:00 MHDA- 2021/11/24 06:00 CRDT- 2020/09/10 05:38 PHST- 2020/01/17 00:00 [received] PHST- 2020/08/27 00:00 [accepted] PHST- 2020/09/11 06:00 [pubmed] PHST- 2021/11/24 06:00 [medline] PHST- 2020/09/10 05:38 [entrez] AID - 10.1007/s43032-020-00308-1 [pii] AID - 10.1007/s43032-020-00308-1 [doi] PST - ppublish SO - Reprod Sci. 2021 Mar;28(3):877-887. doi: 10.1007/s43032-020-00308-1. Epub 2020 Sep 9.