PMID- 32909489
OWN - NLM
STAT- MEDLINE
DCOM- 20201127
LR  - 20210312
IS  - 1743-5404 (Electronic)
IS  - 1743-5390 (Print)
IS  - 1743-5390 (Linking)
VI  - 14
IP  - 8
DP  - 2020 Oct
TI  - Evaluation of the skin-sensitizing potential of gold nanoparticles and the impact 
      of established dermal sensitivity on the pulmonary immune response to various 
      forms of gold.
PG  - 1096-1117
LID - 10.1080/17435390.2020.1808107 [doi]
AB  - Gold nanoparticles (AuNP) are largely biocompatible; however, many studies have 
      demonstrated their potential to modulate various immune cell functions. The 
      potential allergenicity of AuNP remains unclear despite the recognition of gold 
      as a common contact allergen. In these studies, AuNP (29 nm) dermal sensitization 
      potential was assessed via Local Lymph Node Assay (LLNA). Soluble gold (III) 
      chloride (AuCl(3)) caused lymph node (LN) expansion (SI 10.9), whereas bulk 
      particles (Au, 942 nm) and AuNP did not. Next, the pulmonary immune effects of 
      AuNP (10, 30, 90 microg) were assessed 1, 4, and 8 days post-aspiration. All markers 
      of lung injury and inflammation remained unaltered, but a dose-responsive 
      increase in LN size was observed. Finally, mice were dermally-sensitized to 
      AuCl(3) then aspirated once, twice, or three times with Au or AuNP in doses 
      normalized for mass or surface area (SA) to assess the impact of existing contact 
      sensitivity to gold on lung immune responses. Sensitized animals exhibited 
      enhanced responsivity to the metal, wherein subsequent immune alterations were 
      largely conserved with respect to dose SA. The greatest increase in 
      bronchoalveolar lavage (BAL) lymphocyte number was observed in the high dose 
      group - simultaneous to preferential expansion of BAL/LN CD8+ T-cells. 
      Comparatively, the lower SA-based doses of Au/AuNP caused more modest elevations 
      in BAL lymphocyte influx (predominantly CD4+ phenotype), exposure-dependent 
      increases in serum IgE, and selective expansion/activation of LN CD4+ T-cells and 
      B-cells. Overall, these findings suggest that AuNP are unlikely to cause 
      sensitization; however, established contact sensitivity to gold may increase 
      immune responsivity following pulmonary AuNP exposure.
FAU - Roach, K A
AU  - Roach KA
AD  - Allergy and Clinical Immunology Branch (ACIB), National Institute of Occupational 
      Safety and Health (NIOSH), Morgantown, WV, USA.
FAU - Anderson, S E
AU  - Anderson SE
AD  - Allergy and Clinical Immunology Branch (ACIB), National Institute of Occupational 
      Safety and Health (NIOSH), Morgantown, WV, USA.
FAU - Stefaniak, A B
AU  - Stefaniak AB
AD  - Respiratory Health Division (RHD), National Institute of Occupational Safety and 
      Health (NIOSH), Morgantown, WV, USA.
FAU - Shane, H L
AU  - Shane HL
AD  - Allergy and Clinical Immunology Branch (ACIB), National Institute of Occupational 
      Safety and Health (NIOSH), Morgantown, WV, USA.
FAU - Boyce, G R
AU  - Boyce GR
AD  - Allergy and Clinical Immunology Branch (ACIB), National Institute of Occupational 
      Safety and Health (NIOSH), Morgantown, WV, USA.
FAU - Roberts, J R
AU  - Roberts JR
AD  - Allergy and Clinical Immunology Branch (ACIB), National Institute of Occupational 
      Safety and Health (NIOSH), Morgantown, WV, USA.
LA  - eng
GR  - CC999999/ImCDC/Intramural CDC HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20200910
PL  - England
TA  - Nanotoxicology
JT  - Nanotoxicology
JID - 101233132
RN  - 0 (Allergens)
RN  - 0 (Blood Proteins)
RN  - 0 (Cytokines)
RN  - 0 (Gold Compounds)
RN  - 11118-27-7 (gold chloride)
RN  - 7440-57-5 (Gold)
SB  - IM
MH  - Allergens/*toxicity
MH  - Animals
MH  - Blood Proteins/metabolism
MH  - Bronchoalveolar Lavage Fluid/cytology/immunology
MH  - Cytokines/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Gold/*toxicity
MH  - Gold Compounds/*toxicity
MH  - Local Lymph Node Assay
MH  - Lung/*drug effects/immunology
MH  - Lymph Nodes/drug effects/immunology
MH  - Metal Nanoparticles/*toxicity
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Particle Size
MH  - Skin/*drug effects/immunology
MH  - Surface Properties
PMC - PMC7945003
MID - NIHMS1672922
OTO - NOTNLM
OT  - Nanotoxicology
OT  - allergic contact dermatitis
OT  - allergic sensitization
OT  - gold nanoparticles
OT  - pulmonary immune response
COIS- Disclosure statement No potential conflict of interest was reported by the 
      author(s).
EDAT- 2020/09/11 06:00
MHDA- 2020/11/28 06:00
PMCR- 2021/03/10
CRDT- 2020/09/10 08:41
PHST- 2020/09/11 06:00 [pubmed]
PHST- 2020/11/28 06:00 [medline]
PHST- 2020/09/10 08:41 [entrez]
PHST- 2021/03/10 00:00 [pmc-release]
AID - 10.1080/17435390.2020.1808107 [doi]
PST - ppublish
SO  - Nanotoxicology. 2020 Oct;14(8):1096-1117. doi: 10.1080/17435390.2020.1808107. 
      Epub 2020 Sep 10.