PMID- 32910787
OWN - NLM
STAT- MEDLINE
DCOM- 20210419
LR  - 20231207
IS  - 1945-4589 (Electronic)
IS  - 1945-4589 (Linking)
VI  - 12
IP  - 20
DP  - 2020 Sep 10
TI  - Long non-coding RNA DLEUI promotes papillary thyroid carcinoma progression by 
      sponging miR-421 and increasing ROCK1 expression.
PG  - 20127-20138
LID - 10.18632/aging.103642 [doi]
AB  - We investigated the role of long non-coding RNA DLEU1 (deleted in lymphocytic 
      leukemia 1) in the progression of papillary thyroid carcinoma (PTC). DLEU1 levels 
      were higher in PTC cell lines (BHP5-16, TPC-1,8505C, and SW1736) and patient 
      tissues (n=54) than in a human thyroid follicular epithelial cell line 
      (Nthy-ori3-1) or adjacent normal thyroid tissues. High DLEU1 expression 
      correlated positively with lymph node metastasis and advanced clinical stages in 
      PTC patients. Bioinformatics, dual luciferase reporter, and RNA pulldown assays 
      confirmed that DLEU1 directly binds to miR-421. Moreover, bioinformatics and dual 
      luciferase reporter assays showed that miR-421 directly binds to the 
      3'untranslated region of the rho-related coiled-coil kinase 1 (ROCK1) in TPC-1 
      cells. PTC patient tissues and cell lines showed high ROCK1 mRNA and protein 
      levels as well as low miR-421 levels. CCK-8, flow cytometry, wound healing, and 
      Transwell invasion assays demonstrated that DLEU1 silencing decreases TPC-1 cell 
      proliferation, survival and progression, but they can be rescued by miR-421 
      knockdown or ROCK1 overexpression. DLEU1 knockdown in TPC-1 cells decreased in 
      vivo xenograft tumor size and weight compared to controls in nude mice. These 
      findings demonstrate that DLEU1 promotes PTC progression by sponging miR-421 and 
      increasing ROCK1 expression.
FAU - Li, Rui
AU  - Li R
AD  - Department of Thyroid Surgery, The First Hospital of Jilin University, Changchun 
      130021, P.R. China.
FAU - Wan, Taihu
AU  - Wan T
AD  - Department of Division of Interventional Radiology, China-Japan Union Hospital of 
      Jilin University, Changchun 130033, China.
FAU - Qu, Jie
AU  - Qu J
AD  - Department of VIP Unit, China-Japan Union Hospital of Jilin University, Changchun 
      130033, China.
FAU - Yu, Yang
AU  - Yu Y
AD  - Department of General Surgery, China-Japan Union Hospital of Jilin University, 
      Changchun 130033, China.
FAU - Zheng, Ruipeng
AU  - Zheng R
AD  - Department of Interventional Therapy, The First Hospital of Jilin University, 
      Changchun 130021, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20200910
PL  - United States
TA  - Aging (Albany NY)
JT  - Aging
JID - 101508617
RN  - 0 (MIRN421 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - EC 2.7.11.1 (ROCK1 protein, human)
RN  - EC 2.7.11.1 (rho-Associated Kinases)
SB  - IM
EIN - Aging (Albany NY). 2023 Jan 31;15(2):595-596. PMID: 36719851
MH  - Animals
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Disease Progression
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Lymphatic Metastasis
MH  - Male
MH  - Mice, Nude
MH  - MicroRNAs/genetics/*metabolism
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - RNA, Long Noncoding/genetics/*metabolism
MH  - Signal Transduction
MH  - Thyroid Cancer, Papillary/*enzymology/genetics/secondary
MH  - Thyroid Neoplasms/*enzymology/genetics/pathology
MH  - Tumor Burden
MH  - rho-Associated Kinases/genetics/*metabolism
PMC - PMC7655200
OTO - NOTNLM
OT  - DLEU1
OT  - ROCK1
OT  - miR-421
OT  - papillary thyroid carcinoma
COIS- CONFLICTS OF INTEREST: The authors declare that there are no conflicts of 
      interest.
EDAT- 2020/09/11 06:00
MHDA- 2021/04/20 06:00
PMCR- 2020/10/31
CRDT- 2020/09/10 17:09
PHST- 2019/02/03 00:00 [received]
PHST- 2020/06/22 00:00 [accepted]
PHST- 2020/09/11 06:00 [pubmed]
PHST- 2021/04/20 06:00 [medline]
PHST- 2020/09/10 17:09 [entrez]
PHST- 2020/10/31 00:00 [pmc-release]
AID - 103642 [pii]
AID - 10.18632/aging.103642 [doi]
PST - ppublish
SO  - Aging (Albany NY). 2020 Sep 10;12(20):20127-20138. doi: 10.18632/aging.103642. 
      Epub 2020 Sep 10.