PMID- 32912133
OWN - NLM
STAT- MEDLINE
DCOM- 20210412
LR  - 20210412
IS  - 1471-2164 (Electronic)
IS  - 1471-2164 (Linking)
VI  - 21
IP  - 1
DP  - 2020 Sep 10
TI  - Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in 
      Crassostrea gigas infected by Ostreid herpesvirus 1.
PG  - 620
LID - 10.1186/s12864-020-07026-7 [doi]
LID - 620
AB  - BACKGROUND: Since 2008, the aquaculture production of Crassostrea gigas was 
      heavily affected by mass mortalities associated to Ostreid herpesvirus 1 (OsHV-1) 
      microvariants worldwide. Transcriptomic studies revealed the major antiviral 
      pathways of the oyster immune response while other findings suggested that also 
      small non-coding RNAs (sncRNA) such as microRNAs might act as key regulators of 
      the oyster response against OsHV-1. To explore the explicit connection between 
      small non-coding and protein-coding transcripts, we performed paired whole 
      transcriptome analysis of sncRNA and messenger RNA (mRNA) in six oysters selected 
      for different intensities of OsHV-1 infection. RESULTS: The mRNA profiles of the 
      naturally infected oysters were mostly governed by the transcriptional activity 
      of OsHV-1, with several differentially expressed genes mapping to the interferon, 
      toll, apoptosis, and pro-PO pathways. In contrast, miRNA profiles suggested more 
      complex regulatory mechanisms, with 15 differentially expressed miRNAs (DE-miRNA) 
      pointing to a possible modulation of the host response during OsHV-1 infection. 
      We predicted 68 interactions between DE-miRNAs and oyster 3'-UTRs, but only few 
      of them involved antiviral genes. The sncRNA reads assigned to OsHV-1 rather 
      resembled mRNA degradation products, suggesting the absence of genuine viral 
      miRNAs. CONCLUSIONS: We provided data describing the miRNAome during OsHV-1 
      infection in C. gigas. This information can be used to understand the role of 
      miRNAs in healthy and diseased oysters, to identify new targets for functional 
      studies and, eventually to disentangle cause and effect relationships during 
      viral infections in marine mollusks.
FAU - Rosani, Umberto
AU  - Rosani U
AUID- ORCID: 0000-0003-0685-1618
AD  - Department of Biology, University of Padova, 35121, Padova, Italy. 
      umberto.rosani@awi.de.
AD  - Coastal Ecology Section, AWI - Alfred Wegener Institute Helmholtz Centre for 
      Polar and Marine Research, Wadden Sea Station Sylt, 25992, List, Germany. 
      umberto.rosani@awi.de.
FAU - Abbadi, Miriam
AU  - Abbadi M
AD  - Istituto Zooprofilattico delle Venezie, Legnaro, Italy.
FAU - Green, Timothy
AU  - Green T
AD  - Centre for Shellfish Research & Department of Fisheries and Aquaculture, 
      Vancouver Island University, Nanaimo, BC, V9R 5S5, Canada.
FAU - Bai, Chang-Ming
AU  - Bai CM
AD  - Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Qingdao, 266071, China.
FAU - Turolla, Edoardo
AU  - Turolla E
AD  - CRIM Laboratory - Delta Institute, Goro, Ferrara, Italy.
FAU - Arcangeli, Giuseppe
AU  - Arcangeli G
AD  - Istituto Zooprofilattico delle Venezie, Legnaro, Italy.
FAU - Wegner, K Mathias
AU  - Wegner KM
AD  - Coastal Ecology Section, AWI - Alfred Wegener Institute Helmholtz Centre for 
      Polar and Marine Research, Wadden Sea Station Sylt, 25992, List, Germany.
FAU - Venier, Paola
AU  - Venier P
AD  - Department of Biology, University of Padova, 35121, Padova, Italy. 
      paola.venier@unipd.it.
LA  - eng
PT  - Journal Article
DEP - 20200910
PL  - England
TA  - BMC Genomics
JT  - BMC genomics
JID - 100965258
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Messenger)
RN  - Ostreid herpesvirus 1
SB  - IM
MH  - Animals
MH  - Crassostrea/*genetics/virology
MH  - DNA Viruses/pathogenicity
MH  - Disease Resistance
MH  - *Gene Regulatory Networks
MH  - MicroRNAs/*genetics/metabolism
MH  - RNA, Messenger/*genetics/metabolism
MH  - Transcriptome
PMC - PMC7488030
OTO - NOTNLM
OT  - ADAR
OT  - C. gigas
OT  - OsHV-1
OT  - Oyster
OT  - miRNA
OT  - miRNAome
COIS- The authors declare that they have no competing interests.
EDAT- 2020/09/12 06:00
MHDA- 2021/04/13 06:00
PMCR- 2020/09/10
CRDT- 2020/09/11 05:30
PHST- 2020/03/30 00:00 [received]
PHST- 2020/08/25 00:00 [accepted]
PHST- 2020/09/11 05:30 [entrez]
PHST- 2020/09/12 06:00 [pubmed]
PHST- 2021/04/13 06:00 [medline]
PHST- 2020/09/10 00:00 [pmc-release]
AID - 10.1186/s12864-020-07026-7 [pii]
AID - 7026 [pii]
AID - 10.1186/s12864-020-07026-7 [doi]
PST - epublish
SO  - BMC Genomics. 2020 Sep 10;21(1):620. doi: 10.1186/s12864-020-07026-7.