PMID- 32913414
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1348-589X (Print)
IS  - 1349-0923 (Electronic)
IS  - 1348-589X (Linking)
VI  - 45
IP  - 3
DP  - 2020 Aug 20
TI  - Biochemical characterization of NADH:FMN oxidoreductase HcbA3 from Nocardioides 
      sp. PD653 in catalyzing aerobic HCB dechlorination.
PG  - 125-131
LID - 10.1584/jpestics.D20-23 [doi]
AB  - Nocardioides sp. PD653 genes hcbA1, hcbA2, and hcbA3 encode enzymes that catalyze 
      the oxidative dehalogenation of hexachlorobenzene (HCB), which is one of the most 
      recalcitrant persistent organic pollutants (POPs). In this study, HcbA1, HcbA2, 
      and HcbA3 were heterologously expressed and characterized. Among the flavin 
      species tested, HcbA3 showed the highest affinity for FMN with a K (d) value of 
      0.75+/-0.17 microM. Kinetic assays revealed that HcbA3 followed a ping-pong bi-bi 
      mechanism for the reduction of flavins. The K (m) for NADH and FMN was 
      51.66+/-11.58 microM and 4.43+/-0.69 microM, respectively. For both NADH and FMN, the V (max) 
      and k (cat) were 2.21+/-0.86 microM and 66.74+/-5.91 sec(-1), respectively. We also 
      successfully reconstituted the oxidative dehalogenase reaction in vitro, which 
      consisted of HcbA1, HcbA3, FMN, and NADH, suggesting that HcbA3 may be the 
      partner reductase component for HcbA1 in Nocardioides sp. PD653.
CI  - (c) Pesticide Science Society of Japan 2020. This is an open access article 
      distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 
      4.0 International (CC BY-NC-ND 4.0) License 
      (https://creativecommons.org/licenses/by-nc-nd/4.0/).
FAU - Ito, Koji
AU  - Ito K
AD  - Hazardous Chemical Division, Institute for Agro-Environmental Sciences, National 
      Agriculture and Food Research Organisation, Kannondai, Tsukuba-city, Ibaraki, 
      Japan.
FAU - Takagi, Kazuhiro
AU  - Takagi K
AD  - Hazardous Chemical Division, Institute for Agro-Environmental Sciences, National 
      Agriculture and Food Research Organisation, Kannondai, Tsukuba-city, Ibaraki, 
      Japan.
FAU - Kataoka, Ryota
AU  - Kataoka R
AD  - Department of Environmental Sciences, University of Yamanashi, Kofu-city, 
      Yamanashi, Japan.
FAU - Kiyota, Hiromasa
AU  - Kiyota H
AD  - Graduate School of Environmental and Life Science, Okayama University, Tsushima, 
      Okayama-city, Okayama, Japan.
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - J Pestic Sci
JT  - Journal of pesticide science
JID - 101580833
PMC - PMC7453296
OTO - NOTNLM
OT  - HcbA3
OT  - Nocardioides sp. PD653
OT  - TC-FDM
OT  - flavin reductase
OT  - hexachlorobenzene
EDAT- 2020/09/12 06:00
MHDA- 2020/09/12 06:01
PMCR- 2020/08/20
CRDT- 2020/09/11 05:43
PHST- 2020/09/11 05:43 [entrez]
PHST- 2020/09/12 06:00 [pubmed]
PHST- 2020/09/12 06:01 [medline]
PHST- 2020/08/20 00:00 [pmc-release]
AID - 10.1584/jpestics.D20-23 [doi]
PST - ppublish
SO  - J Pestic Sci. 2020 Aug 20;45(3):125-131. doi: 10.1584/jpestics.D20-23.