PMID- 32913529
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 12
IP  - 8
DP  - 2020
TI  - Fluorescence in situ hybridization for WWTR1-CAMTA1 has higher sensitivity and 
      specificity for epithelioid hemangioendothelioma diagnosis.
PG  - 4561-4568
AB  - Epithelioid hemangioendothelioma (EHE) is a rare medium-to-low-grade malignant 
      vascular tumor characterized by vascular differentiation along with specific 
      morphological and genetic alterations. Approximately 90% and 5% of EHE cases are 
      associated with the WWTR1-CAMTA1 and YAP1/TFE3 fusion gene, respectively. 
      Therefore, nuclear CAMTA1 protein expression is considered to be an effective 
      marker for EHE diagnosis. However, the specificity and reliability of this 
      approach have recently been put into question. The purpose of this study was to 
      compare the detection of CAMTA1 expression in cases of EHE and histologic mimics 
      using fluorescence in situ hybridization (FISH) and conventional protein 
      immunohistochemistry via hematoxylin and eosin staining. Fifteen EHE and 37 
      histologic mimic samples were immunohistochemically stained with polyclonal 
      anti-CAMTA1 antibody to evaluate the nuclear protein expression level of CAMTA1. 
      In addition, 15 EHE samples and 10 vascular tumor samples were subjected to FISH 
      to detect the WWTR1-CAMTA1 fusion gene. Histologically, EHE typically showed a 
      mucous hyaline or cartilaginous stroma, often forming a primitive vascular lumen, 
      and expressed vascular endothelial markers. Twelve of the 15 EHE samples showed 
      positive nuclear CAMTA1 expression with immunohistochemistry, whereas six of the 
      37 histologic mimics showed positive nuclear expression. FISH detected a 
      red-green signal fusion in 14 of the 15 cases of EHE, but in none of the 10 
      vascular tumors. These results indicate that CAMTA1 is an effective and useful 
      EHE marker, but that FISH fusion gene detection has better diagnostic value and 
      clinical significance.
CI  - AJTR Copyright (c) 2020.
FAU - Yang, Panpan
AU  - Yang P
AD  - Department of Pathology, The First Affiliated Hospital of Bengbu Medical College, 
      Bengbu Medical College Anhui, China.
FAU - Zhang, Shan
AU  - Zhang S
AD  - Department of Pathology, The First Affiliated Hospital of Bengbu Medical College, 
      Bengbu Medical College Anhui, China.
FAU - Yu, Chaowen
AU  - Yu C
AD  - Department of Vascular Surgery, The First Affiliated Hospital of Bengbu Medical 
      College Bengbu 233030, Anhui, China.
FAU - Yan, Wentian
AU  - Yan W
AD  - Department of Pathology, The First Affiliated Hospital of Bengbu Medical College, 
      Bengbu Medical College Anhui, China.
FAU - Yang, Ningning
AU  - Yang N
AD  - Department of Pathology, The First Affiliated Hospital of Bengbu Medical College, 
      Bengbu Medical College Anhui, China.
FAU - Li, Nan
AU  - Li N
AD  - Department of Pathology, The First Affiliated Hospital of Bengbu Medical College, 
      Bengbu Medical College Anhui, China.
FAU - Huang, Yuchen
AU  - Huang Y
AD  - Department of Pathology, The First Affiliated Hospital of Bengbu Medical College, 
      Bengbu Medical College Anhui, China.
FAU - Chen, Hongchun
AU  - Chen H
AD  - Department of Pathology, The First Affiliated Hospital of Bengbu Medical College, 
      Bengbu Medical College Anhui, China.
FAU - Cai, Zhaogen
AU  - Cai Z
AD  - Department of Pathology, The First Affiliated Hospital of Bengbu Medical College, 
      Bengbu Medical College Anhui, China.
FAU - Zhao, Yan
AU  - Zhao Y
AD  - Department of Pathology, The First Affiliated Hospital of Bengbu Medical College, 
      Bengbu Medical College Anhui, China.
FAU - Qin, Yanzi
AU  - Qin Y
AD  - Department of Pathology, The First Affiliated Hospital of Bengbu Medical College, 
      Bengbu Medical College Anhui, China.
FAU - Gong, Xiaomeng
AU  - Gong X
AD  - Department of Pathology, The First Affiliated Hospital of Bengbu Medical College, 
      Bengbu Medical College Anhui, China.
FAU - Feng, Zhenzhong
AU  - Feng Z
AD  - Department of Pathology, The First Affiliated Hospital of Bengbu Medical College, 
      Bengbu Medical College Anhui, China.
LA  - eng
PT  - Journal Article
DEP - 20200815
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC7476104
OTO - NOTNLM
OT  - CAMTA1
OT  - FISH
OT  - epithelioid hemangioendothelioma
OT  - immunohistochemistry
COIS- None.
EDAT- 2020/09/12 06:00
MHDA- 2020/09/12 06:01
PMCR- 2020/08/15
CRDT- 2020/09/11 05:44
PHST- 2020/01/08 00:00 [received]
PHST- 2020/07/03 00:00 [accepted]
PHST- 2020/09/11 05:44 [entrez]
PHST- 2020/09/12 06:00 [pubmed]
PHST- 2020/09/12 06:01 [medline]
PHST- 2020/08/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2020 Aug 15;12(8):4561-4568. eCollection 2020.