PMID- 32914944
OWN - NLM
STAT- MEDLINE
DCOM- 20211213
LR  - 20211214
IS  - 1764-1489 (Print)
IS  - 1764-1489 (Linking)
VI  - 53
IP  - 3
DP  - 2021 May
TI  - Immune signature of CCR7(+) central memory T cells associates with disease 
      severity and Immunoglobulin E in bronchial asthma.
PG  - 115-127
LID - 10.23822/EurAnnACI.1764-1489.168 [doi]
AB  - Objective. CD4(+)T cell subtypes are the central orchestrators of airway 
      inflammation in bronchialasthma (BA); however, the mechanisms that regulate their 
      accumulation in asthmatic airways are still a challenging subject. In addition, 
      neutrophils play a significant role in the development of airway remodeling and 
      their presence may influence clinical presentation of BA being linked to the 
      development of severe BA. Neutrophils have also been found to acquireantigen 
      presenting functions, enabling them to directly activate T cells. The study aimed 
      to evaluate the possible association of chemokine receptor 7 (CCR7)(+) memory 
      CD4(+)T cells andCCR4(+) effector T cells with disease severity and 
      immunoglobulin E (IgE) production as well as to explore the relationship between 
      these cells and neutrophil function in both allergic andnon-allergic asthmatic 
      patients. Methods. Flow cytometry was used to determine the expression of 
      different T cell subset phenotypes (CCR7 memory CD4(+) and CCR4(+)T cells using 
      anti-human CD3, CD4, CD45RO, CCR4 and CCR7 monoclonal antibodies) utilizing 
      peripheral blood mononuclear cells (PBMCs) isolated from 78 allergic asthmatic 
      patients, 41 non-allergic asthmatic patients, and 40 healthy individuals. 
      Moreover, neutrophils' phagocytic activity was assessed by ingestion of candida 
      particles. Results. We demonstrated increased percentages of CCR7(+) memory 
      CD4(+)T cells and CCR4(+)CD4(+)T cells in patients compared to control, where 
      this up regulation was significantly higher in allergic than non-allergic 
      asthmatic patients. Additionally, these cells were negatively correlated with 
      improved pulmonary tests and significantly associated with disease severity 
      scores and IgE levels. The neutrophil phagocytic activity was markedly increased 
      in patients compared to control, showing a significant positive correlation with 
      disease severity. Conclusions. These findings suggest that increased CCR4(+) 
      CD4(+) T cells and CCR7(+) memory CD4(+) T cells (Tcm) may be associated with BA 
      severity, especially in allergic BA patients and can potentially contribute to 
      the rational design of new therapeutic approaches for asthma in the future.
FAU - Moaaz, M
AU  - Moaaz M
AD  - Department of Immunology and Allergy, Medical Research Institute, Alexandria 
      University, Alexandria, Egypt.
FAU - Youssry, S
AU  - Youssry S
AD  - Department of Immunology and Allergy, Medical Research Institute, Alexandria 
      University, Alexandria, Egypt.
FAU - Baess, A
AU  - Baess A
AD  - Department of Chest Diseases, Faculty of Medicine, Alexandria University, 
      Alexandria, Egypt.
FAU - Abed, A
AU  - Abed A
AD  - College of Health and Medical Technology, Baghdad, Iraq.
FAU - Moaaz, M
AU  - Moaaz M
AD  - Department of Human Physiology, Clinical Respiratory Physiology Unit, Medical 
      Research Institute, Alexandria University, Alexandria, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20200911
PL  - Italy
TA  - Eur Ann Allergy Clin Immunol
JT  - European annals of allergy and clinical immunology
JID - 101466614
RN  - 0 (CCR7 protein, human)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Receptors, CCR7)
RN  - 0 (Receptors, Chemokine)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Asthma/diagnosis/*immunology
MH  - CD4-Positive T-Lymphocytes/*immunology
MH  - Female
MH  - Humans
MH  - *Immunoglobulin E
MH  - Inflammation Mediators/metabolism
MH  - Leukocytes, Mononuclear
MH  - Male
MH  - *Memory T Cells
MH  - Middle Aged
MH  - Receptors, CCR7
MH  - Receptors, Chemokine/*analysis
MH  - Severity of Illness Index
MH  - T-Lymphocyte Subsets/*immunology
OTO - NOTNLM
OT  - Asthma
OT  - CCR4
OT  - CCR7
OT  - IgE
OT  - allergic
OT  - phagocytic activity
EDAT- 2020/09/12 06:00
MHDA- 2021/12/15 06:00
CRDT- 2020/09/11 10:58
PHST- 2020/09/12 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
PHST- 2020/09/11 10:58 [entrez]
AID - 10.23822/EurAnnACI.1764-1489.168 [doi]
PST - ppublish
SO  - Eur Ann Allergy Clin Immunol. 2021 May;53(3):115-127. doi: 
      10.23822/EurAnnACI.1764-1489.168. Epub 2020 Sep 11.