PMID- 32915230
OWN - NLM
STAT- MEDLINE
DCOM- 20210329
LR  - 20210329
IS  - 1573-4935 (Electronic)
IS  - 0144-8463 (Print)
IS  - 0144-8463 (Linking)
VI  - 40
IP  - 9
DP  - 2020 Sep 30
TI  - Emodin protects against intestinal and lung injury induced by acute intestinal 
      injury by modulating SP-A and TLR4/NF-kappaB pathway.
LID - 10.1042/BSR20201605 [doi]
LID - BSR20201605
AB  - OBJECTIVE: Our aim was to investigate the effect of emodin on intestinal and lung 
      injury induced by acute intestinal injury in rats and explore potential molecular 
      mechanisms. METHODS: Healthy male Sprague-Dawley (SD) rats were randomly divided 
      into five groups (n=10, each group): normal group; saline group; acute intestinal 
      injury model group; model + emodin group; model+NF-kappaB inhibitor pynolidine 
      dithiocarbamate (PDTC) group. Histopathological changes in intestine/lung tissues 
      were observed by Hematoxylin and Eosin (H&E) and terminal deoxynucleotidyl 
      transferase biotin-dUTP nick-end labeling (TUNEL) staining. Serum IKBalpha, p-IKBalpha, 
      surfactant protein-A (SP-A) and toll-like receptor 4 (TLR4) levels were examined 
      using enzyme-linked immunosorbent assay (ELISA). RT-qPCR was performed to detect 
      the mRNA expression levels of IKBalpha, SP-A and TLR4 in intestine/lung tissues. 
      Furthermore, the protein expression levels of IKBalpha, p-IKBalpha, SP-A and TLR4 were 
      detected by Western blot. RESULTS: The pathological injury of intestinal/lung 
      tissues was remarkedly ameliorated in models treated with emodin and PDTC. 
      Furthermore, the intestinal/lung injury scores were significantly decreased after 
      emodin or PDTC treatment. TUNEL results showed that both emodin and PDTC 
      treatment distinctly attenuated the apoptosis of intestine/lung tissues induced 
      by acute intestinal injury. At the mRNA level, emodin significantly increased the 
      expression levels of SP-A and decreased the expression levels of IKBalpha and TLR4 in 
      intestine/lung tissues. According to ELISA and Western blot, emodin remarkedly 
      inhibited the expression of p-IKBalpha protein and elevated the expression of SP-A 
      and TLR4 in serum and intestine/lung tissues induced by acute intestinal injury. 
      CONCLUSION: Our findings suggested that emodin could protect against intestinal 
      and lung injury induced by acute intestinal injury by modulating SP-A and 
      TLR4/NF-kappaB pathway.
CI  - (c) 2020 The Author(s).
FAU - Qian, Jingli
AU  - Qian J
AD  - Department of Respiration, The Second Affiliated Hospital of Zhejiang Chinese 
      Medical University, Hangzhou 310005, Zhejiang, China.
FAU - Li, Guoping
AU  - Li G
AD  - Department of Respiration, Tongde Hospital of Zhejiang Province, Hangzhou 310012, 
      Zhejiang, China.
FAU - Jin, Xiaosheng
AU  - Jin X
AD  - Department of Respiration, Tongde Hospital of Zhejiang Province, Hangzhou 310012, 
      Zhejiang, China.
FAU - Ma, Chunfang
AU  - Ma C
AD  - Clinical Laboratory, Zhejiang Provincial People's Hospital, Hangzhou 310014, 
      Zhejiang, China.
FAU - Cai, Wanru
AU  - Cai W
AD  - Department of Respiration, The Second Affiliated Hospital of Zhejiang Chinese 
      Medical University, Hangzhou 310005, Zhejiang, China.
FAU - Jiang, Na
AU  - Jiang N
AD  - Department of Respiration, Tongde Hospital of Zhejiang Province, Hangzhou 310012, 
      Zhejiang, China.
FAU - Zheng, Jisheng
AU  - Zheng J
AD  - Department of Respiration, Tongde Hospital of Zhejiang Province, Hangzhou 310012, 
      Zhejiang, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biosci Rep
JT  - Bioscience reports
JID - 8102797
RN  - 0 (NF-kappa B)
RN  - 0 (Pulmonary Surfactant-Associated Protein A)
RN  - 0 (Pyrrolidines)
RN  - 0 (Thiocarbamates)
RN  - 0 (Tlr4 protein, rat)
RN  - 0 (Toll-Like Receptor 4)
RN  - 25769-03-3 (pyrrolidine dithiocarbamic acid)
RN  - KA46RNI6HN (Emodin)
RN  - Q40Q9N063P (Acetic Acid)
SB  - IM
MH  - Acetic Acid/administration & dosage/toxicity
MH  - Acute Lung Injury/etiology/pathology/*prevention & control
MH  - Animals
MH  - Colon/blood supply/*drug effects/pathology
MH  - Disease Models, Animal
MH  - Emodin/*administration & dosage
MH  - Humans
MH  - Intestinal Mucosa/blood supply/*drug effects/pathology
MH  - Ischemia/chemically induced/complications/*drug therapy
MH  - Lung/drug effects/pathology
MH  - Male
MH  - NF-kappa B/antagonists & inhibitors/metabolism
MH  - Pulmonary Surfactant-Associated Protein A/metabolism
MH  - Pyrrolidines/administration & dosage
MH  - Rats
MH  - Signal Transduction/drug effects
MH  - Thiocarbamates/administration & dosage
MH  - Toll-Like Receptor 4/metabolism
PMC - PMC7517261
OTO - NOTNLM
OT  - acute intestinal injury
OT  - emodin
OT  - lung injury
OT  - surfactant protein-A
OT  - toll-like receptor 4
COIS- The authors declare that there are no competing interests associated with the 
      manuscript.
EDAT- 2020/09/12 06:00
MHDA- 2021/03/30 06:00
PMCR- 2020/09/24
CRDT- 2020/09/11 12:12
PHST- 2020/05/30 00:00 [received]
PHST- 2020/09/02 00:00 [revised]
PHST- 2020/09/10 00:00 [accepted]
PHST- 2020/09/12 06:00 [pubmed]
PHST- 2021/03/30 06:00 [medline]
PHST- 2020/09/11 12:12 [entrez]
PHST- 2020/09/24 00:00 [pmc-release]
AID - 226403 [pii]
AID - BSR20201605 [pii]
AID - 10.1042/BSR20201605 [doi]
PST - ppublish
SO  - Biosci Rep. 2020 Sep 30;40(9):BSR20201605. doi: 10.1042/BSR20201605.