PMID- 32916215
OWN - NLM
STAT- MEDLINE
DCOM- 20210114
LR  - 20220413
IS  - 1873-2399 (Electronic)
IS  - 0301-472X (Print)
IS  - 0301-472X (Linking)
VI  - 90
DP  - 2020 Oct
TI  - Interleukin 7 receptor is required for myeloid cell homeostasis and 
      reconstitution by hematopoietic stem cells.
PG  - 39-45.e3
LID - S0301-472X(20)30359-3 [pii]
LID - 10.1016/j.exphem.2020.09.001 [doi]
AB  - Respiratory diseases are a leading cause of death worldwide, with vulnerability 
      to disease varying greatly between individuals. The reasons underlying disease 
      susceptibility are unknown, but there is often a variable immune response in 
      lungs often. Recently, we identified a surprising novel role for the interleukin 
      7 receptor (IL7R), a primarily lymphoid-associated regulator, in fetal-specified, 
      lung-resident macrophage development. Here, we report that traditional, 
      hematopoietic stem cell-derived myeloid cells in the adult lung, peripheral 
      blood, and bone marrow also depend on IL7R expression. Using single- and 
      double-germline knockout models, we found that eosinophil numbers were reduced on 
      deletion of IL7Ralpha. We then employed two Cre recombinase models in lineage tracing 
      experiments to test whether these cells developed through an IL7Ralpha+ pathway. 
      Despite the impact of IL7Ralpha deletion, IL7R-Cre labeled only a minimal fraction of 
      eosinophils. We therefore examined the intrinsic versus extrinsic requirement for 
      IL7R in the production of eosinophils using reciprocal hematopoietic stem cell 
      transplantation assays. These assays revealed that extrinsic, but not 
      eosinophil-intrinsic, IL7R is required for eosinophil reconstitution by HSCs in 
      the adult lung. To determine which external factors may be influencing eosinophil 
      development and survival, we performed a cytokine array analysis between 
      wild-type and IL7Ralpha-deficient mice and found several differentially regulated 
      proteins. These findings expand on our previous report that IL7R is required not 
      only for proper lymphoid cell development and homeostasis, but also for myeloid 
      cell homeostasis in tissues.
CI  - Copyright (c) 2020 ISEH -- Society for Hematology and Stem Cells. Published by 
      Elsevier Inc. All rights reserved.
FAU - Cool, Taylor
AU  - Cool T
AD  - Institute for the Biology of Stem Cells, University of California-Santa Cruz, 
      Santa Cruz, CA; Program in Biomedical Sciences and Engineering, Department of 
      Molecular, Cell, and Developmental Biology, University of California-Santa Cruz, 
      Santa Cruz, CA.
FAU - Worthington, Atesh
AU  - Worthington A
AD  - Institute for the Biology of Stem Cells, University of California-Santa Cruz, 
      Santa Cruz, CA; Program in Biomedical Sciences and Engineering, Department of 
      Molecular, Cell, and Developmental Biology, University of California-Santa Cruz, 
      Santa Cruz, CA.
FAU - Poscablo, Donna
AU  - Poscablo D
AD  - Institute for the Biology of Stem Cells, University of California-Santa Cruz, 
      Santa Cruz, CA; Program in Biomedical Sciences and Engineering, Department of 
      Molecular, Cell, and Developmental Biology, University of California-Santa Cruz, 
      Santa Cruz, CA.
FAU - Hussaini, Adeel
AU  - Hussaini A
AD  - Institute for the Biology of Stem Cells, University of California-Santa Cruz, 
      Santa Cruz, CA.
FAU - Forsberg, E Camilla
AU  - Forsberg EC
AD  - Institute for the Biology of Stem Cells, University of California-Santa Cruz, 
      Santa Cruz, CA; Biomolecular Engineering, University of California-Santa Cruz, 
      Santa Cruz, CA. Electronic address: cforsber@ucsc.edu.
LA  - eng
GR  - F31 HL151199/HL/NHLBI NIH HHS/United States
GR  - R01 DK100917/DK/NIDDK NIH HHS/United States
GR  - R25 GM058903/GM/NIGMS NIH HHS/United States
PT  - Journal Article
DEP - 20200908
PL  - Netherlands
TA  - Exp Hematol
JT  - Experimental hematology
JID - 0402313
RN  - 0 (Receptors, Interleukin-7)
SB  - IM
MH  - Animals
MH  - Female
MH  - Hematopoietic Stem Cells/*immunology
MH  - Homeostasis/genetics/*immunology
MH  - Lung/cytology/*immunology
MH  - Lymphocytes/cytology/immunology
MH  - Male
MH  - Mice
MH  - Mice, Knockout
MH  - Myeloid Cells/cytology/*immunology
MH  - Receptors, Interleukin-7/genetics/*immunology
MH  - Signal Transduction/genetics/*immunology
PMC - PMC7951140
MID - NIHMS1630871
COIS- Competing interests: The authors have no conflicting financial interests.
EDAT- 2020/09/12 06:00
MHDA- 2021/01/15 06:00
PMCR- 2021/10/01
CRDT- 2020/09/11 20:11
PHST- 2020/05/02 00:00 [received]
PHST- 2020/09/01 00:00 [revised]
PHST- 2020/09/02 00:00 [accepted]
PHST- 2020/09/12 06:00 [pubmed]
PHST- 2021/01/15 06:00 [medline]
PHST- 2020/09/11 20:11 [entrez]
PHST- 2021/10/01 00:00 [pmc-release]
AID - S0301-472X(20)30359-3 [pii]
AID - 10.1016/j.exphem.2020.09.001 [doi]
PST - ppublish
SO  - Exp Hematol. 2020 Oct;90:39-45.e3. doi: 10.1016/j.exphem.2020.09.001. Epub 2020 
      Sep 8.