PMID- 32917613
OWN - NLM
STAT- MEDLINE
DCOM- 20220412
LR  - 20220412
IS  - 2375-2548 (Electronic)
IS  - 2375-2548 (Linking)
VI  - 6
IP  - 36
DP  - 2020 Sep
TI  - Revealing the in vivo growth and division patterns of mouse gut bacteria.
LID - 10.1126/sciadv.abb2531 [doi]
LID - eabb2531
AB  - Current techniques for studying gut microbiota are unable to answer some 
      important microbiology questions, like how different bacteria grow and divide in 
      the gut. We propose a method that integrates the use of sequential d-amino 
      acid-based in vivo metabolic labeling with fluorescence in situ hybridization 
      (FISH), for characterizing the growth and division patterns of gut bacteria. 
      After sequentially administering two d-amino acid-based probes containing 
      different fluorophores to mice by gavage, the resulting dual-labeled 
      peptidoglycans provide temporal information on cell wall synthesis of gut 
      bacteria. Following taxonomic identification with FISH probes, the growth and 
      division patterns of the corresponding bacterial taxa, including species that 
      cannot be cultured separately in vitro, are revealed. Our method offers a facile 
      yet powerful tool for investigating the in vivo growth dynamics of the bacterial 
      gut microbiota, which will advance our understanding of bacterial cytology and 
      facilitate elucidation of the basic microbiology of this gut "dark matter."
CI  - Copyright (c) 2020 The Authors, some rights reserved; exclusive licensee American 
      Association for the Advancement of Science. No claim to original U.S. Government 
      Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 
      (CC BY-NC).
FAU - Lin, Liyuan
AU  - Lin L
AUID- ORCID: 0000-0002-9202-0652
AD  - Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid 
      Chemistry and Nanomedicine, Renji Hospital, Shanghai Jiao Tong University School 
      of Medicine, Shanghai 200127, China.
FAU - Wu, Qiuyue
AU  - Wu Q
AD  - Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid 
      Chemistry and Nanomedicine, Renji Hospital, Shanghai Jiao Tong University School 
      of Medicine, Shanghai 200127, China.
AD  - The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Key 
      Laboratory for Chemical Biology of Fujian Province State Key Laboratory of 
      Physical Chemistry of Solid Surfaces, Department of Chemical Biology, College of 
      Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
FAU - Song, Jia
AU  - Song J
AUID- ORCID: 0000-0002-0122-8360
AD  - Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid 
      Chemistry and Nanomedicine, Renji Hospital, Shanghai Jiao Tong University School 
      of Medicine, Shanghai 200127, China.
FAU - Du, Yahui
AU  - Du Y
AD  - The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Key 
      Laboratory for Chemical Biology of Fujian Province State Key Laboratory of 
      Physical Chemistry of Solid Surfaces, Department of Chemical Biology, College of 
      Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
FAU - Gao, Juan
AU  - Gao J
AUID- ORCID: 0000-0001-6608-0312
AD  - Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid 
      Chemistry and Nanomedicine, Renji Hospital, Shanghai Jiao Tong University School 
      of Medicine, Shanghai 200127, China.
FAU - Song, Yanling
AU  - Song Y
AD  - Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid 
      Chemistry and Nanomedicine, Renji Hospital, Shanghai Jiao Tong University School 
      of Medicine, Shanghai 200127, China.
AD  - The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Key 
      Laboratory for Chemical Biology of Fujian Province State Key Laboratory of 
      Physical Chemistry of Solid Surfaces, Department of Chemical Biology, College of 
      Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
FAU - Wang, Wei
AU  - Wang W
AUID- ORCID: 0000-0002-5539-4532
AD  - Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid 
      Chemistry and Nanomedicine, Renji Hospital, Shanghai Jiao Tong University School 
      of Medicine, Shanghai 200127, China. wwang@shsmu.edu.cn cyyang@xmu.edu.cn.
FAU - Yang, Chaoyong
AU  - Yang C
AUID- ORCID: 0000-0002-2374-5342
AD  - Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid 
      Chemistry and Nanomedicine, Renji Hospital, Shanghai Jiao Tong University School 
      of Medicine, Shanghai 200127, China. wwang@shsmu.edu.cn cyyang@xmu.edu.cn.
AD  - The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Key 
      Laboratory for Chemical Biology of Fujian Province State Key Laboratory of 
      Physical Chemistry of Solid Surfaces, Department of Chemical Biology, College of 
      Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200904
PL  - United States
TA  - Sci Adv
JT  - Science advances
JID - 101653440
RN  - 0 (Amino Acids)
RN  - 0 (Fluorescent Dyes)
SB  - IM
MH  - Amino Acids
MH  - Animals
MH  - *Bacteria
MH  - Fluorescent Dyes/metabolism
MH  - *Gastrointestinal Microbiome
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
PMC - PMC7473744
EDAT- 2020/09/13 06:00
MHDA- 2022/04/13 06:00
PMCR- 2020/09/04
CRDT- 2020/09/12 05:28
PHST- 2020/02/11 00:00 [received]
PHST- 2020/07/13 00:00 [accepted]
PHST- 2020/09/12 05:28 [entrez]
PHST- 2020/09/13 06:00 [pubmed]
PHST- 2022/04/13 06:00 [medline]
PHST- 2020/09/04 00:00 [pmc-release]
AID - 6/36/eabb2531 [pii]
AID - abb2531 [pii]
AID - 10.1126/sciadv.abb2531 [doi]
PST - epublish
SO  - Sci Adv. 2020 Sep 4;6(36):eabb2531. doi: 10.1126/sciadv.abb2531. Print 2020 Sep.