PMID- 32918398
OWN - NLM
STAT- MEDLINE
DCOM- 20211129
LR  - 20211129
IS  - 1744-7917 (Electronic)
IS  - 1672-9609 (Linking)
VI  - 28
IP  - 5
DP  - 2021 Oct
TI  - Molecular basis of the silkworm mutant re(l) causing red egg color and embryonic 
      death.
PG  - 1290-1299
LID - 10.1111/1744-7917.12871 [doi]
AB  - The coloration and hatchability of insect eggs can affect individual and 
      population survival. However, few genetic loci have been documented to affect 
      both traits, and the genes involved in regulating these two traits are unclear. 
      The silkworm recessive mutant re(l) shows both red egg color and embryo 
      mortality. We studied the molecular basis of the re(l) phenotype formation. 
      Through genetic analysis, gene screening and sequencing, we found that two 
      closely linked genes, BGIBMGA003497 (Bm-re) and BGIBMGA003697 (BmSema1a), control 
      egg color and embryo mortality, respectively. Six base pairs of the Bm-re gene 
      are deleted in its open reading frame, and BmSema1a is expressed at abnormally 
      low levels in mutant re(l) . BmSema1a gene function verification was performed 
      using RNA interference and clustered randomly interspersed palindromic repeats 
      (CRISPR)/CRISPR-associate protein 9. Deficiency of the BmSema1a gene can cause 
      the death of silkworm embryos. This study revealed the molecular basis of 
      silkworm re(l) mutant formation and indicated that the Sema1a gene is essential 
      for insect embryo development.
CI  - (c) 2020 Institute of Zoology, Chinese Academy of Sciences.
FAU - Luo, Jiang-Wen
AU  - Luo JW
AD  - State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural 
      Biology and Genetic Breeding, College of Biotechnology, Southwest University, 
      Chongqing, 400715, China.
FAU - An, Er-Xia
AU  - An EX
AD  - State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural 
      Biology and Genetic Breeding, College of Biotechnology, Southwest University, 
      Chongqing, 400715, China.
FAU - Lu, Ya-Ru
AU  - Lu YR
AD  - State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural 
      Biology and Genetic Breeding, College of Biotechnology, Southwest University, 
      Chongqing, 400715, China.
FAU - Yang, Ling
AU  - Yang L
AD  - State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural 
      Biology and Genetic Breeding, College of Biotechnology, Southwest University, 
      Chongqing, 400715, China.
FAU - Gai, Ting-Ting
AU  - Gai TT
AD  - State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural 
      Biology and Genetic Breeding, College of Biotechnology, Southwest University, 
      Chongqing, 400715, China.
FAU - He, Song-Zhen
AU  - He SZ
AD  - State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural 
      Biology and Genetic Breeding, College of Biotechnology, Southwest University, 
      Chongqing, 400715, China.
FAU - Wu, Song-Yuan
AU  - Wu SY
AD  - State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural 
      Biology and Genetic Breeding, College of Biotechnology, Southwest University, 
      Chongqing, 400715, China.
FAU - Hu, Hai
AU  - Hu H
AD  - State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural 
      Biology and Genetic Breeding, College of Biotechnology, Southwest University, 
      Chongqing, 400715, China.
FAU - Li, Chun-Lin
AU  - Li CL
AD  - State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural 
      Biology and Genetic Breeding, College of Biotechnology, Southwest University, 
      Chongqing, 400715, China.
FAU - Lu, Cheng
AU  - Lu C
AD  - State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural 
      Biology and Genetic Breeding, College of Biotechnology, Southwest University, 
      Chongqing, 400715, China.
FAU - Tong, Xiao-Ling
AU  - Tong XL
AD  - State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural 
      Biology and Genetic Breeding, College of Biotechnology, Southwest University, 
      Chongqing, 400715, China.
FAU - Dai, Fang-Yin
AU  - Dai FY
AUID- ORCID: 0000-0002-0215-2177
AD  - State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural 
      Biology and Genetic Breeding, College of Biotechnology, Southwest University, 
      Chongqing, 400715, China.
LA  - eng
GR  - CARS-18-ZJ0102/Earmarked Fund for China Agriculture Research System/
GR  - 31830094/National Natural Science Foundation of China/
GR  - 2013AA102507/Hi-Tech Research and Development 863 Program of China Grant/
PT  - Journal Article
DEP - 20201013
PL  - Australia
TA  - Insect Sci
JT  - Insect science
JID - 101266965
RN  - 0 (Insect Proteins)
RN  - 0 (Semaphorins)
SB  - IM
MH  - Animals
MH  - *Bombyx/embryology/genetics
MH  - Embryo, Nonmammalian
MH  - Embryonic Development/genetics
MH  - *Insect Proteins/genetics
MH  - Ovum/*pathology
MH  - Phenotype
MH  - Pigmentation
MH  - Semaphorins/*genetics
OTO - NOTNLM
OT  - BmSema1a
OT  - CRISPR/Cas9
OT  - embryonic mortality
OT  - red egg
OT  - silkworm
EDAT- 2020/09/13 06:00
MHDA- 2021/11/30 06:00
CRDT- 2020/09/12 05:36
PHST- 2020/08/26 00:00 [revised]
PHST- 2020/05/21 00:00 [received]
PHST- 2020/09/01 00:00 [accepted]
PHST- 2020/09/13 06:00 [pubmed]
PHST- 2021/11/30 06:00 [medline]
PHST- 2020/09/12 05:36 [entrez]
AID - 10.1111/1744-7917.12871 [doi]
PST - ppublish
SO  - Insect Sci. 2021 Oct;28(5):1290-1299. doi: 10.1111/1744-7917.12871. Epub 2020 Oct 
      13.