PMID- 32918493
OWN - NLM
STAT- MEDLINE
DCOM- 20210622
LR  - 20210622
IS  - 1348-0421 (Electronic)
IS  - 0385-5600 (Linking)
VI  - 64
IP  - 11
DP  - 2020 Nov
TI  - Abiotrophia defectiva adhere to saliva-coated hydroxyapatite beads via 
      interactions between salivary proline-rich-proteins and bacterial 
      glyceraldehyde-3-phosphate dehydrogenase.
PG  - 719-729
LID - 10.1111/1348-0421.12848 [doi]
AB  - Abiotrophia defectiva is a species of nutritionally variant streptococci that is 
      found in human saliva and dental plaques and that has been associated with 
      infective endocarditis. In our previous study, it was found that A. defectiva 
      could bind specifically to saliva-coated hydroxyapatite beads (SHA). This study 
      identified a cell surface component of A. defectiva that promotes adherence to 
      SHA beads. The binding of A. defectiva to SHA was reduced in the presence of 
      antibodies against human proline-rich protein (PRP); these results suggested that 
      PRP may be a critical component mediating interactions between A. defectiva and 
      the salivary pellicle. Two-dimensional gel electrophoresis of whole A. defectiva 
      cells followed by Far-Western blotting was conducted by probing with synthetic 
      peptides analogous to the binding region of PRP known as PRP-C. The results 
      indicate that an A. defectiva protein of 37 kDa interacts with PRP-C. The results 
      of amino-terminal sequencing of the adhesive A. defectiva protein revealed 
      significant similarity to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). 
      Recombinant GAPDH bound to immobilized PRP-C in a dose-dependent manner and 
      binding of A. defectiva to SHA or to PRP was reduced in the presence of 
      anti-GAPDH antiserum. Western blotting or electron immunomicroscopic observations 
      with anti-GAPDH antiserum revealed that this protein was expressed in both 
      cytosolic and cell wall fractions. These results suggest that A. defectiva could 
      specifically bind to PRP via interactions with cell surface GAPDH; the findings 
      suggest a mechanism underlying A. defectiva-mediated adherence to saliva-coated 
      tooth surfaces.
CI  - (c) 2020 The Societies and John Wiley & Sons Australia, Ltd.
FAU - Sasaki, Minoru
AU  - Sasaki M
AUID- ORCID: 0000-0002-6105-2424
AD  - Division of Molecular Microbiology, Department of Microbiology, Iwate Medical 
      University, Shiwa-gun, Iwate, Japan.
FAU - Kodama, Yoshitoyo
AU  - Kodama Y
AD  - Division of Molecular Microbiology, Department of Microbiology, Iwate Medical 
      University, Shiwa-gun, Iwate, Japan.
FAU - Shimoyama, Yu
AU  - Shimoyama Y
AD  - Division of Molecular Microbiology, Department of Microbiology, Iwate Medical 
      University, Shiwa-gun, Iwate, Japan.
FAU - Ishikawa, Taichi
AU  - Ishikawa T
AD  - Division of Molecular Microbiology, Department of Microbiology, Iwate Medical 
      University, Shiwa-gun, Iwate, Japan.
FAU - Tajika, Shihoko
AU  - Tajika S
AD  - Division of Molecular Microbiology, Department of Microbiology, Iwate Medical 
      University, Shiwa-gun, Iwate, Japan.
FAU - Kimura, Shigenobu
AU  - Kimura S
AD  - Division of Molecular Microbiology, Department of Microbiology, Iwate Medical 
      University, Shiwa-gun, Iwate, Japan.
LA  - eng
PT  - Journal Article
DEP - 20201008
PL  - Australia
TA  - Microbiol Immunol
JT  - Microbiology and immunology
JID - 7703966
RN  - 0 (Peptides)
RN  - 0 (Salivary Proline-Rich Proteins)
RN  - 91D9GV0Z28 (Durapatite)
RN  - 9DLQ4CIU6V (Proline)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
RN  - Abiotrophia defectiva
SB  - IM
MH  - Abiotrophia/genetics/*metabolism
MH  - Amino Acid Sequence
MH  - *Bacterial Adhesion
MH  - Durapatite/*metabolism
MH  - Escherichia coli/genetics
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/chemistry/genetics/*metabolism
MH  - Humans
MH  - Peptides
MH  - Proline
MH  - Saliva/*microbiology
MH  - Salivary Proline-Rich Proteins/*metabolism
MH  - Streptococcus/metabolism
OTO - NOTNLM
OT  - Abiotrophia defectiva
OT  - GAPDH
OT  - colonization
OT  - proline rich proteins
OT  - salivary pellicle
EDAT- 2020/09/13 06:00
MHDA- 2021/06/23 06:00
CRDT- 2020/09/12 08:34
PHST- 2020/05/31 00:00 [received]
PHST- 2020/09/02 00:00 [revised]
PHST- 2020/09/09 00:00 [accepted]
PHST- 2020/09/13 06:00 [pubmed]
PHST- 2021/06/23 06:00 [medline]
PHST- 2020/09/12 08:34 [entrez]
AID - 10.1111/1348-0421.12848 [doi]
PST - ppublish
SO  - Microbiol Immunol. 2020 Nov;64(11):719-729. doi: 10.1111/1348-0421.12848. Epub 
      2020 Oct 8.