PMID- 32919703
OWN - NLM
STAT- MEDLINE
DCOM- 20210318
LR  - 20211204
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 533
IP  - 1
DP  - 2020 Nov 26
TI  - mTOR - Mediated protein synthesis by inhibiting protein catabolism in Chinese 
      perch (Siniperca chuatsi).
PG  - 23-29
LID - S0006-291X(20)31706-X [pii]
LID - 10.1016/j.bbrc.2020.08.107 [doi]
AB  - Activation of the mechanistic target of rapamycin (mTOR) pathway is known to 
      promote protein synthesis by enhancing mRNA translation. However, there have been 
      few literatures on the effect of mTOR on protein metabolism in non-mammals. The 
      main source of ammonia in fish comes from protein catabolism. The key step of 
      protein catabolism involves the deamination and/or transamination of amino acids. 
      This study is aimed to explore the mechanism underlying mTOR pathway influencing 
      protein retention from the perspective of protein catabolism. Chinese perch were 
      fasted for 24 h and divided into 4 groups randomly before intracerebroventricular 
      (ICV) injection: (1) control group for leucine; (2) leucine group; (3) control 
      group for leucine and rapamycin; (4) leucine and rapamycin group. Food intake was 
      equivalent between each control and treatment groups at each time point (0.5, 4, 
      12 and 24 h post-injection). Ammonia-N excretion rate, blood glucose, S6 
      phosphorylation level, and expression of relative genes of protein catabolism 
      (GDH, AMPD, AST, ALT) were determined. The results indicated that the pS6 level 
      was increased, and that the ammonia-N excretion rate, blood glucose, and mRNA 
      level of protein catabolism genes (GDH and AMPD) were significantly decreased 
      after injection with leucine, while those changes were reversed after injection 
      with leucine and rapamycin. Our study not only reveals the mechanism by which 
      mTOR mediates protein synthesis by inhibiting protein catabolism in Chinese 
      perch, but also provides reference for improving the utilization of feed protein.
CI  - Copyright (c) 2020 Elsevier Inc. All rights reserved.
FAU - Li, Jiao
AU  - Li J
AD  - College of Fisheries, Chinese Perch Research Center, Huazhong Agricultural 
      University, Wuhan, 430070, China; Key Lab of Freshwater Animal Breeding, Ministry 
      of Agriculture and Rural Affair/ Hubei Engineering Technology Research Center for 
      Fish Breeding and Healthy Aquaculture, Wuhan, 430070, China.
FAU - Zhang, Yanpeng
AU  - Zhang Y
AD  - College of Fisheries, Chinese Perch Research Center, Huazhong Agricultural 
      University, Wuhan, 430070, China; Key Lab of Freshwater Animal Breeding, Ministry 
      of Agriculture and Rural Affair/ Hubei Engineering Technology Research Center for 
      Fish Breeding and Healthy Aquaculture, Wuhan, 430070, China.
FAU - Liang, Xu-Fang
AU  - Liang XF
AD  - College of Fisheries, Chinese Perch Research Center, Huazhong Agricultural 
      University, Wuhan, 430070, China; Key Lab of Freshwater Animal Breeding, Ministry 
      of Agriculture and Rural Affair/ Hubei Engineering Technology Research Center for 
      Fish Breeding and Healthy Aquaculture, Wuhan, 430070, China. Electronic address: 
      xufang_liang@hotmail.com.
FAU - He, Shan
AU  - He S
AD  - College of Fisheries, Chinese Perch Research Center, Huazhong Agricultural 
      University, Wuhan, 430070, China; Key Lab of Freshwater Animal Breeding, Ministry 
      of Agriculture and Rural Affair/ Hubei Engineering Technology Research Center for 
      Fish Breeding and Healthy Aquaculture, Wuhan, 430070, China.
FAU - Tang, Shulin
AU  - Tang S
AD  - College of Fisheries, Chinese Perch Research Center, Huazhong Agricultural 
      University, Wuhan, 430070, China; Key Lab of Freshwater Animal Breeding, Ministry 
      of Agriculture and Rural Affair/ Hubei Engineering Technology Research Center for 
      Fish Breeding and Healthy Aquaculture, Wuhan, 430070, China.
FAU - Li, Ling
AU  - Li L
AD  - College of Fisheries, Chinese Perch Research Center, Huazhong Agricultural 
      University, Wuhan, 430070, China; Key Lab of Freshwater Animal Breeding, Ministry 
      of Agriculture and Rural Affair/ Hubei Engineering Technology Research Center for 
      Fish Breeding and Healthy Aquaculture, Wuhan, 430070, China.
FAU - Chen, Xu
AU  - Chen X
AD  - College of Fisheries, Chinese Perch Research Center, Huazhong Agricultural 
      University, Wuhan, 430070, China; Key Lab of Freshwater Animal Breeding, Ministry 
      of Agriculture and Rural Affair/ Hubei Engineering Technology Research Center for 
      Fish Breeding and Healthy Aquaculture, Wuhan, 430070, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200909
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Fish Proteins)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - GMW67QNF9C (Leucine)
SB  - IM
MH  - Animals
MH  - Fish Proteins/*metabolism
MH  - Leucine/metabolism
MH  - Perches/*metabolism
MH  - *Protein Biosynthesis
MH  - Proteolysis
MH  - TOR Serine-Threonine Kinases/*metabolism
OTO - NOTNLM
OT  - Leucine
OT  - Protein catabolism
OT  - Siniperca chuatsi
OT  - ammonia-N excretion Rate
OT  - mTOR
COIS- Declaration of competing interest The authors declare that the research was 
      conducted in the absence of any commercial or financial relationships that could 
      be construed as potential conflicts of interest.
EDAT- 2020/09/14 06:00
MHDA- 2021/03/19 06:00
CRDT- 2020/09/13 20:26
PHST- 2020/08/20 00:00 [received]
PHST- 2020/08/26 00:00 [accepted]
PHST- 2020/09/14 06:00 [pubmed]
PHST- 2021/03/19 06:00 [medline]
PHST- 2020/09/13 20:26 [entrez]
AID - S0006-291X(20)31706-X [pii]
AID - 10.1016/j.bbrc.2020.08.107 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2020 Nov 26;533(1):23-29. doi: 
      10.1016/j.bbrc.2020.08.107. Epub 2020 Sep 9.