PMID- 32920086 OWN - NLM STAT- MEDLINE DCOM- 20210615 LR - 20210615 IS - 1873-4847 (Electronic) IS - 0955-2863 (Linking) VI - 86 DP - 2020 Dec TI - Erica multiflora extract rich in quercetin-3-O-glucoside and kaempferol-3-O-glucoside alleviates high fat and fructose diet-induced fatty liver disease by modulating metabolic and inflammatory pathways in Wistar rats. PG - 108490 LID - S0955-2863(20)30522-2 [pii] LID - 10.1016/j.jnutbio.2020.108490 [doi] AB - The wide morbidity of obesity has heightened interest in providing natural and safe compounds to maintain optimal health. The present study was designed to determine the chemical constituents and the effects of methanol leaf extract from Erica multiflora (M-EML) on mitigating high-fat and high-fructose diet (HFFD)-induced metabolic syndrome (MS). LC-MS/MS characterization of M-EML allowed the identification of 14 secondary metabolites and showed that quercetin-3-O-glucoside and kaempferol-3-O-glucoside were the main compounds of our extract. In the in vivo study, the oral administration of M-EML (250 mg/kg) during the last 4 weeks of the experimentation alleviated HFFD-induced obesity, insulin resistance (IR) and cardiovascular diseases. Thus, M-EML treatment significantly normalized body and liver weight, allowed to a sharp decline in plasma levels of TC, TG and LDL-c by 32%, 35% and 66%, respectively. Moreover, hepatic enzymes, total and direct bilirubin, lipase and uric acid levels have been diminished in treated group. Histopathology of the liver confirmed the changes induced by HFFD and the hepatoprotective effect of M-EML. The supply of M-EML reduced NO production and cellular lysosomal enzyme activity by 44% and 60%, respectively compared to HFFD. Besides, M-EML showed decreased pro-inflammatory cytokines levels (259.5+/-47.35 pg/ml and 56.08+/-1.56 pg/ml) of TNF-alpha and IL-6, respectively. In addition, M-EML reduced liver malondialdehyde (MDA) content and enhanced superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities. In contrast, these enzymatic activities have been disrupted in HFFD rats. Overall, M-EML prevented obesity through the modulation of metabolic syndrome, reducing inflammation and promoting antioxidant enzymes activities. CI - Copyright (c) 2020 Elsevier Inc. All rights reserved. FAU - Khlifi, Rihab AU - Khlifi R AD - Unity of Bioactive and Natural Substances and Biotechnology UR17ES49, Faculty of Dental Medicine, University of Monastir, Avicenna Street, 5000 Monastir, Tunisia; Higher Institute of Biotechnology of Monastir, Avenue Tahar Hadded, BP 74, 5000 Monastir, Tunisia. Electronic address: rihabkhlifi@gmail.com. FAU - Dhaouefi, Zaineb AU - Dhaouefi Z AD - Unity of Bioactive and Natural Substances and Biotechnology UR17ES49, Faculty of Dental Medicine, University of Monastir, Avicenna Street, 5000 Monastir, Tunisia. FAU - Toumia, Imene Ben AU - Toumia IB AD - Unity of Bioactive and Natural Substances and Biotechnology UR17ES49, Faculty of Dental Medicine, University of Monastir, Avicenna Street, 5000 Monastir, Tunisia; Faculty of Pharmacy, University of Monastir, Avicenna Street, 5000 Monastir, Tunisia. FAU - Lahmar, Aida AU - Lahmar A AD - Unity of Bioactive and Natural Substances and Biotechnology UR17ES49, Faculty of Dental Medicine, University of Monastir, Avicenna Street, 5000 Monastir, Tunisia. FAU - Sioud, Fairouz AU - Sioud F AD - Unity of Bioactive and Natural Substances and Biotechnology UR17ES49, Faculty of Dental Medicine, University of Monastir, Avicenna Street, 5000 Monastir, Tunisia; Faculty of Pharmacy, University of Monastir, Avicenna Street, 5000 Monastir, Tunisia. FAU - Bouhajeb, Rim AU - Bouhajeb R AD - Unity of Bioactive and Natural Substances and Biotechnology UR17ES49, Faculty of Dental Medicine, University of Monastir, Avicenna Street, 5000 Monastir, Tunisia; Faculty of Pharmacy, University of Monastir, Avicenna Street, 5000 Monastir, Tunisia. FAU - Bellalah, Ahlem AU - Bellalah A AD - Department of Pathology, Fattouma Bourguiba University Hospital, Monastir, Tunisia. FAU - Chekir-Ghedira, Leila AU - Chekir-Ghedira L AD - Unity of Bioactive and Natural Substances and Biotechnology UR17ES49, Faculty of Dental Medicine, University of Monastir, Avicenna Street, 5000 Monastir, Tunisia. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20200911 PL - United States TA - J Nutr Biochem JT - The Journal of nutritional biochemistry JID - 9010081 RN - 0 (Dietary Fats) RN - 0 (Glucosides) RN - 0 (Kaempferols) RN - 0 (Monosaccharides) RN - 0 (Plant Extracts) RN - 0 (Reactive Oxygen Species) RN - 0 (kaempferol-3-O-glucoside) RN - 0 (quercetin 3'-O-glucoside) RN - 30237-26-4 (Fructose) RN - 9IKM0I5T1E (Quercetin) RN - Y4S76JWI15 (Methanol) SB - IM MH - Animals MH - Diet, High-Fat/adverse effects MH - Dietary Fats MH - Ericales/*chemistry MH - Fatty Liver/*metabolism MH - Fructose/adverse effects MH - Glucosides/chemistry/*pharmacology MH - Inflammation/metabolism MH - Insulin Resistance MH - Kaempferols/*pharmacology MH - Liver/metabolism MH - Male MH - Metabolic Syndrome/metabolism MH - Methanol/chemistry MH - Monosaccharides/*pharmacology MH - Oxidative Stress MH - Plant Extracts/*pharmacology MH - Quercetin/*analogs & derivatives/pharmacology MH - Rats MH - Rats, Wistar MH - Reactive Oxygen Species/metabolism MH - Tandem Mass Spectrometry OTO - NOTNLM OT - Erica multiflora OT - High-fat-fructose diet OT - Inflammation OT - LC-MS/MS OT - Metabolic syndrome OT - Oxidative stress COIS- Declaration of competing interest The authors declare that they have no competing interests. EDAT- 2020/09/14 06:00 MHDA- 2021/06/16 06:00 CRDT- 2020/09/13 20:29 PHST- 2020/03/09 00:00 [received] PHST- 2020/06/28 00:00 [revised] PHST- 2020/08/13 00:00 [accepted] PHST- 2020/09/14 06:00 [pubmed] PHST- 2021/06/16 06:00 [medline] PHST- 2020/09/13 20:29 [entrez] AID - S0955-2863(20)30522-2 [pii] AID - 10.1016/j.jnutbio.2020.108490 [doi] PST - ppublish SO - J Nutr Biochem. 2020 Dec;86:108490. doi: 10.1016/j.jnutbio.2020.108490. Epub 2020 Sep 11.