PMID- 32920249
OWN - NLM
STAT- MEDLINE
DCOM- 20210730
LR  - 20210730
IS  - 1873-2232 (Electronic)
IS  - 0378-4320 (Linking)
VI  - 221
DP  - 2020 Oct
TI  - Associations between foetal size and ovarian development in the pig.
PG  - 106589
LID - S0378-4320(20)30461-9 [pii]
LID - 10.1016/j.anireprosci.2020.106589 [doi]
AB  - It is estimated that intra-uterine growth restricted piglets represent 25 % of 
      the total number of piglets born. Growth restricted female pigs have impaired 
      reproductive performance postnatally. HHowever, when during gestation this 
      phenotype arises is not known. With this study, the aim was to improve the 
      understanding of foetal ovarian development in normal and small foetuses 
      throughout gestation. Female Large White X Landrace foetuses were obtained at 
      gestational day (GD) 45, 60 and 90 (n = 5-6 litters/GD). Histological analysis of 
      GATA4 stained foetal ovaries at GD60 and 90 indicated there were fewer primary 
      follicles (P </= 0.05) in the foetuses weighing the least compared to those with a 
      weight similar to the mean for the litter (CTMLW) at GD90. Plasma oestradiol 
      concentrations were less in the foetuses with lesser weights compared with 
      greater weight foetuses at GD90 (P </= 0.05). The RNA was extracted from ovaries of 
      the lesser weight and CTMLW foetuses at GD45, 60 and 90 and qPCR was performed to 
      quantify relative abundance of 12 candidate mRNAs for which encoded proteins that 
      modulate ovarian function and development. Gestational changes in relative 
      abundances of CD31, PTGFR, SPP1 and VEGFA mRNA transcripts were observed. 
      Relative abundance of KI67 (P = 0.066) and P53 (P </= 0.05) was less in ovaries of 
      the lesser weight compared to CTMLW foetuses at GD60. There was a lesser relative 
      abundance of PTGFR mRNA transcript in ovaries from the foetuses with lesser 
      weight compared to CTMLW foetuses at GD45 and 60 (P </= 0.05). These findings 
      indicate that postnatal differences in the reproductive potential of growth 
      restricted females are programmed early in gestation. It is hoped that further 
      investigation will improve the understanding of the relationship between prenatal 
      reproductive development and postnatal reproductive performance.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Stenhouse, Claire
AU  - Stenhouse C
AD  - Functional Genetics and Development Division, The Roslin Institute and Royal 
      (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, UK. 
      Electronic address: clairestenhouse@tamu.edu.
FAU - Cortes-Araya, Yennifer
AU  - Cortes-Araya Y
AD  - Functional Genetics and Development Division, The Roslin Institute and Royal 
      (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, UK.
FAU - Hogg, Charis O
AU  - Hogg CO
AD  - Functional Genetics and Development Division, The Roslin Institute and Royal 
      (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, UK.
FAU - Donadeu, F Xavier
AU  - Donadeu FX
AD  - Functional Genetics and Development Division, The Roslin Institute and Royal 
      (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, UK.
FAU - Ashworth, Cheryl J
AU  - Ashworth CJ
AD  - Functional Genetics and Development Division, The Roslin Institute and Royal 
      (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, UK.
LA  - eng
PT  - Journal Article
DEP - 20200902
PL  - Netherlands
TA  - Anim Reprod Sci
JT  - Animal reproduction science
JID - 7807205
RN  - 0 (Platelet Endothelial Cell Adhesion Molecule-1)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Prostaglandin)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 106441-73-0 (Osteopontin)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Animals
MH  - Estradiol/genetics/metabolism
MH  - Female
MH  - *Fetal Development
MH  - Fetal Growth Retardation/*veterinary
MH  - Gene Expression Regulation, Developmental/physiology
MH  - Osteopontin/genetics/metabolism
MH  - Ovary/*growth & development
MH  - Platelet Endothelial Cell Adhesion Molecule-1/genetics/metabolism
MH  - Pregnancy
MH  - *Pregnancy, Animal
MH  - RNA, Messenger
MH  - Receptors, Prostaglandin/genetics/metabolism
MH  - Swine/*physiology
MH  - Vascular Endothelial Growth Factor A/genetics/metabolism
OTO - NOTNLM
OT  - Foetal growth
OT  - Intrauterine growth restriction (IUGR)
OT  - Ovary
OT  - Porcine
EDAT- 2020/09/14 06:00
MHDA- 2021/07/31 06:00
CRDT- 2020/09/13 20:31
PHST- 2020/06/24 00:00 [received]
PHST- 2020/08/25 00:00 [revised]
PHST- 2020/08/26 00:00 [accepted]
PHST- 2020/09/14 06:00 [pubmed]
PHST- 2021/07/31 06:00 [medline]
PHST- 2020/09/13 20:31 [entrez]
AID - S0378-4320(20)30461-9 [pii]
AID - 10.1016/j.anireprosci.2020.106589 [doi]
PST - ppublish
SO  - Anim Reprod Sci. 2020 Oct;221:106589. doi: 10.1016/j.anireprosci.2020.106589. 
      Epub 2020 Sep 2.