PMID- 32921061
OWN - NLM
STAT- MEDLINE
DCOM- 20210621
LR  - 20210621
IS  - 1520-5827 (Electronic)
IS  - 0743-7463 (Linking)
VI  - 36
IP  - 39
DP  - 2020 Oct 6
TI  - How Nanotopography-Induced Conformational Changes of Fibrinogen Affect Platelet 
      Adhesion and Activation.
PG  - 11573-11580
LID - 10.1021/acs.langmuir.0c02094 [doi]
AB  - The conformational state of adsorbed human plasma fibrinogen (HPF) has been 
      recognized as the determinant factor in platelet adhesion and thrombus formation 
      on blood-contacting biomaterials. Studies have highlighted the ability to control 
      the HPF conformation merely by tailoring surface nanotopographical features. 
      However, a clear relationship between the conformational changes of adsorbed HPF 
      and the degree of platelet adhesion and activation achieved with different 
      surface nanotopographies is still unclear. Here, we examined HPF assembly 
      characteristics on nanostructured polybutene-1 (PB-1) surfaces with nanosized 
      lamellar crystals (LCs), needle-like crystals (NLCs), and a nanostructured 
      high-density polyethylene (HDPE) surface with shish-kebab crystals (SKCs), at a 
      biologically relevant HPF concentration. By exposing the nanostructured surfaces 
      with preadsorbed HPF to human platelets, significant differences in platelet 
      response on LCs/SKCs and NLCs were identified. The former presented a uniform 
      monolayer in the advanced stage of activation, whereas the latter exhibited 
      minimal adhesion and the early stage of activation. Distinct platelet response 
      was related to the postadsorption conformational changes in HPF, which were 
      confirmed by topography-dependent shifts of the amide I band in attenuated total 
      reflection-Fourier transform infrared (ATR-FTIR) analysis. Supported by atomic 
      force microscopy (AFM) characterization, we propose that the mechanism behind the 
      nanotopography-induced HPF conformation is driven by the interplay between the 
      aspect ratios of polymeric crystals and HPF. From the biomedical perspective, our 
      work reveals that surface structuring in a nanoscale size regime can provide a 
      fine-tuning mechanism to manipulate HPF conformation, which can be exploited for 
      the design of thromboresistant biomaterials surfaces.
FAU - Firkowska-Boden, Izabela
AU  - Firkowska-Boden I
AUID- ORCID: 0000-0001-9924-0247
AD  - Chair of Materials Science, Otto Schott Institute of Materials Research, Faculty 
      of Physics and Astronomy, Friedrich Schiller University Jena, Lobdergraben 32, 
      07743 Jena, Germany.
FAU - Helbing, Christian
AU  - Helbing C
AD  - Chair of Materials Science, Otto Schott Institute of Materials Research, Faculty 
      of Physics and Astronomy, Friedrich Schiller University Jena, Lobdergraben 32, 
      07743 Jena, Germany.
FAU - Dauben, Thomas J
AU  - Dauben TJ
AD  - Chair of Materials Science, Otto Schott Institute of Materials Research, Faculty 
      of Physics and Astronomy, Friedrich Schiller University Jena, Lobdergraben 32, 
      07743 Jena, Germany.
FAU - Pieper, Maja
AU  - Pieper M
AD  - Chair of Materials Science, Otto Schott Institute of Materials Research, Faculty 
      of Physics and Astronomy, Friedrich Schiller University Jena, Lobdergraben 32, 
      07743 Jena, Germany.
FAU - Jandt, Klaus D
AU  - Jandt KD
AUID- ORCID: 0000-0002-7537-5603
AD  - Chair of Materials Science, Otto Schott Institute of Materials Research, Faculty 
      of Physics and Astronomy, Friedrich Schiller University Jena, Lobdergraben 32, 
      07743 Jena, Germany.
AD  - Jena Center for Soft Matter, Friedrich Schiller University Jena, Philosophenweg 
      7, 07743 Jena, Germany.
AD  - Jena School for Microbial Communication (JSMC), Neugasse 23, 07743 Jena, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200925
PL  - United States
TA  - Langmuir
JT  - Langmuir : the ACS journal of surfaces and colloids
JID - 9882736
RN  - 0 (Biocompatible Materials)
RN  - 9001-32-5 (Fibrinogen)
SB  - IM
MH  - Adsorption
MH  - Biocompatible Materials
MH  - Blood Platelets
MH  - *Fibrinogen
MH  - Humans
MH  - Platelet Activation
MH  - *Platelet Adhesiveness
MH  - Surface Properties
EDAT- 2020/09/15 06:00
MHDA- 2021/06/22 06:00
CRDT- 2020/09/14 05:29
PHST- 2020/09/15 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2020/09/14 05:29 [entrez]
AID - 10.1021/acs.langmuir.0c02094 [doi]
PST - ppublish
SO  - Langmuir. 2020 Oct 6;36(39):11573-11580. doi: 10.1021/acs.langmuir.0c02094. Epub 
      2020 Sep 25.