PMID- 32921644
OWN - NLM
STAT- MEDLINE
DCOM- 20210712
LR  - 20210712
IS  - 1347-7439 (Electronic)
IS  - 0916-7250 (Print)
IS  - 0916-7250 (Linking)
VI  - 82
IP  - 11
DP  - 2020 Nov 12
TI  - Establishment and characterization of a canine sebaceous epithelial cell line 
      derived from an eyelid mass.
PG  - 1577-1584
LID - 10.1292/jvms.20-0179 [doi]
AB  - Little is known about the pathological roles of sebaceous glands in canine skin 
      diseases, as most examinations have been conducted with cultured human sebaceous 
      epithelial cell lines. To our knowledge, there is no available canine sebaceous 
      epithelial cell line. The purpose of this study was to establish a canine 
      sebaceous epithelial cell line and characterize it. An eyelid mass in a dog was 
      surgically resected for treatment, and it was histologically diagnosed as 
      sebaceous epithelioma. Collected tissue was conducted for culture, and the 
      growing epithelial-like cells were passaged. The cells showed continuous 
      proliferation for over 6 months. After 40 passages, the cells were named CMG-1. 
      Lipid droplets in the cytoplasm of CMG-1 cells were confirmed by Oil Red O 
      staining. As reported in studies with human sebaceous epithelial cell lines, 
      lipogenesis in CMG-1 cells was promoted by linoleic acid, whereas transforming 
      growth factor-beta (TGF-beta) suppressed it. Additionally, real-time PCR revealed that 
      the expression levels of chemokines and cytokines, including CC chemokine ligand 
      (CCL)-2, CCL-20, CXCL-10, Tumor necrosis factor-alpha (TNF-alpha), Interleukin (IL)-1alpha, 
      IL-1beta, and IL-8, were significantly increased in CMG-1 cells following treatment 
      with lipopolysaccharide. In conclusion, we successfully established a new canine 
      sebaceous epithelial cell line. Our data indicated that lipogenesis and 
      inflammatory responses were quantitatively evaluable in this cell line. CMG-1 
      cells could be useful for the pathological analysis of sebaceous gland diseases 
      in dogs.
FAU - Matsuda, Akira
AU  - Matsuda A
AD  - Faculty of Veterinary Medicine, Okayama University of Science, 1-3 Ikoinooka, 
      Imabari, Ehime 794-8555, Japan.
FAU - Mitsui, Ikki
AU  - Mitsui I
AD  - Faculty of Veterinary Medicine, Okayama University of Science, 1-3 Ikoinooka, 
      Imabari, Ehime 794-8555, Japan.
FAU - Shimizu, Yuki
AU  - Shimizu Y
AD  - Faculty of Veterinary Medicine, Okayama University of Science, 1-3 Ikoinooka, 
      Imabari, Ehime 794-8555, Japan.
FAU - Kanda, Teppei
AU  - Kanda T
AD  - Faculty of Veterinary Medicine, Okayama University of Science, 1-3 Ikoinooka, 
      Imabari, Ehime 794-8555, Japan.
FAU - Ohnishi, Akihiro
AU  - Ohnishi A
AD  - Faculty of Veterinary Medicine, Okayama University of Science, 1-3 Ikoinooka, 
      Imabari, Ehime 794-8555, Japan.
FAU - Miyabe, Masahiro
AU  - Miyabe M
AD  - Faculty of Veterinary Medicine, Okayama University of Science, 1-3 Ikoinooka, 
      Imabari, Ehime 794-8555, Japan.
FAU - Itoh, Yoshiki
AU  - Itoh Y
AD  - Faculty of Veterinary Medicine, Okayama University of Science, 1-3 Ikoinooka, 
      Imabari, Ehime 794-8555, Japan.
LA  - eng
PT  - Journal Article
DEP - 20200911
PL  - Japan
TA  - J Vet Med Sci
JT  - The Journal of veterinary medical science
JID - 9105360
RN  - 0 (Cytokines)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cytokines/genetics
MH  - Dogs
MH  - Epithelial Cells
MH  - Eyelids
MH  - *Sebaceous Glands
PMC - PMC7719885
OTO - NOTNLM
OT  - cell line
OT  - dog
OT  - meibomian gland
OT  - sebaceous epithelial cell
OT  - sebaceous gland
EDAT- 2020/09/15 06:00
MHDA- 2021/07/13 06:00
PMCR- 2020/11/01
CRDT- 2020/09/14 05:47
PHST- 2020/09/15 06:00 [pubmed]
PHST- 2021/07/13 06:00 [medline]
PHST- 2020/09/14 05:47 [entrez]
PHST- 2020/11/01 00:00 [pmc-release]
AID - 20-0179 [pii]
AID - 10.1292/jvms.20-0179 [doi]
PST - ppublish
SO  - J Vet Med Sci. 2020 Nov 12;82(11):1577-1584. doi: 10.1292/jvms.20-0179. Epub 2020 
      Sep 11.