PMID- 32922080
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220416
IS  - 1179-1322 (Print)
IS  - 1179-1322 (Electronic)
IS  - 1179-1322 (Linking)
VI  - 12
DP  - 2020
TI  - Long Non-Coding RNA TRPM2-AS Promotes Cell Migration and Invasion by Serving as a 
      ceRNA of miR-138 and Inducing SOX4-Mediated EMT in Laryngeal Squamous Cell 
      Carcinoma.
PG  - 7805-7812
LID - 10.2147/CMAR.S265412 [doi]
AB  - BACKGROUND: Laryngeal squamous cell carcinoma (LSCC) is a common type of 
      malignant tumors of larynx, and in this study, we aimed to evaluate the 
      functional role of long non-coding RNA TRPM2-AS in LSCC. METHODS: The expression 
      levels of TRPM2-AS in LSCC tissues and cell lines were detected by RT-qPCR 
      analysis. In vitro functional assays, including MTT assay and transwell assay, 
      were performed to explore the biological effects of TRPM2-AS on LSCC cells. The 
      expression levels of EMT-relevant proteins were detected by Western blot 
      analysis. The interaction between TRPM2-AS and miR-138 in LSCC, predicted by 
      bioinformatic method, was verified by dual-luciferase reporter assay. RESULTS: We 
      observed that TRPM2-AS was highly expressed in human LSCC tissues and cell lines. 
      LSCC patients with advanced clinical stage exhibited higher intratumoral TRPM2-AS 
      expression. The results of functional assays demonstrated that TRPM2-AS knockdown 
      remarkably inhibited the proliferation, migration and invasion of LSCC cells, 
      whereas TRPM2-AS overexpression showed opposite effects. In mechanism, we further 
      observed that TRPM2-AS directly bound to miR-138 and served as competing 
      endogenous RNA (ceRNA), thereby increasing SOX4 expression and promoting EMT in 
      LSCC. The oncogenic effects of TRPM2-AS in LSCC cells were partly diminished by 
      miR-138 restoration. CONCLUSION: In short, our findings provided first evidence 
      that TRPM2-AS is highly expressed and exerts its oncogenic role in LSCC partly by 
      miR-138/SOX4 axis.
CI  - (c) 2020 Wang et al.
FAU - Wang, Ning
AU  - Wang N
AD  - Department of Otorhinolaryngology, Qilu Hospital, Cheeloo College of Medicine, 
      Shandong University, Jinan, Shandong 250012, People's Republic of China.
AD  - Department of Otolaryngology, Qilu Hospital (Qingdao), Cheeloo College of 
      Medicine, Shandong University, Qingdao, Shandong 266035, People's Republic of 
      China.
FAU - Wang, Lei
AU  - Wang L
AD  - Department of Otolaryngology, Qilu Hospital (Qingdao), Cheeloo College of 
      Medicine, Shandong University, Qingdao, Shandong 266035, People's Republic of 
      China.
FAU - Pan, Xinliang
AU  - Pan X
AD  - Department of Otorhinolaryngology, Qilu Hospital, Cheeloo College of Medicine, 
      Shandong University, Jinan, Shandong 250012, People's Republic of China.
AD  - Department of Otolaryngology, Qilu Hospital (Qingdao), Cheeloo College of 
      Medicine, Shandong University, Qingdao, Shandong 266035, People's Republic of 
      China.
AD  - NHC Key Laboratory of Otorhinolaryngology (Shandong University), Jinan, Shandong 
      250012, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20200825
PL  - New Zealand
TA  - Cancer Manag Res
JT  - Cancer management and research
JID - 101512700
PMC - PMC7457742
OTO - NOTNLM
OT  - EMT
OT  - SOX4
OT  - laryngeal squamous cell carcinoma
OT  - long non-coding RNA TRPM2-AS
OT  - miR-138
COIS- The authors report no conflicts of interest for this work.
EDAT- 2020/09/15 06:00
MHDA- 2020/09/15 06:01
PMCR- 2020/08/25
CRDT- 2020/09/14 05:49
PHST- 2020/05/31 00:00 [received]
PHST- 2020/07/27 00:00 [accepted]
PHST- 2020/09/14 05:49 [entrez]
PHST- 2020/09/15 06:00 [pubmed]
PHST- 2020/09/15 06:01 [medline]
PHST- 2020/08/25 00:00 [pmc-release]
AID - 265412 [pii]
AID - 10.2147/CMAR.S265412 [doi]
PST - epublish
SO  - Cancer Manag Res. 2020 Aug 25;12:7805-7812. doi: 10.2147/CMAR.S265412. 
      eCollection 2020.