PMID- 32922544
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240329
IS  - 1837-9664 (Print)
IS  - 1837-9664 (Electronic)
IS  - 1837-9664 (Linking)
VI  - 11
IP  - 20
DP  - 2020
TI  - Surfactin from Bacillus subtilis attenuates ambient air particulate 
      matter-promoted human oral cancer cells metastatic potential.
PG  - 6038-6049
LID - 10.7150/jca.48296 [doi]
AB  - Recently, many studies have indicated that ambient air particulate matter (PM) 
      can increase the risk of oral cancer. The most common malignant tumor in the oral 
      cavity is oral squamous cell carcinoma (OSCC). Usually, cancer cell 
      migration/invasion is the most important cause of cancer mortality. Matrix 
      metalloproteinase-2 (MMP-2) and MMP-9 have been shown to play important roles in 
      regulating metastasis and the tumor microenvironment. Here, we studied the 
      anti-cancer effects of surfactin, a cyclic lipopeptide generated by Bacillus 
      subtilis, on cancer cell migration and invasion. Surfactin suppressed PM-promoted 
      cell migration and invasion and colony formation of SCC4 and SCC25 human oral 
      squamous cell carcinoma cell lines. We observed that PM induced MMP-2 and MMP-9 
      expression, which was inhibited by surfactin. Transfection with p65, p50, c-Jun, 
      c-Fos, p85, p110, Akt, mammalian target of rapamycin (mTOR), or interleukin-6 
      (IL-6) siRNA markedly inhibited PM-induced MMP-2 and MMP-9 expression. Moreover, 
      surfactin could reduce Akt, mTOR, p65, and c-Jun activation and IL-6 secretion 
      induced by PM. Finally, we proved that transfection with Akt, p65, or c-Jun siRNA 
      significantly inhibited PM-induced IL-6 release. Taken together, these results 
      suggest that surfactin functions as a suppressor of PM-induced MMP2/9-dependent 
      oral cancer cell migration and invasion by inhibiting the activation of 
      phosphoinositide 3-kinase (PI3K)/Akt/mTOR and PI3K/Akt/nuclear factor-kappaB (NF-kappaB) 
      and activator protein-1 (AP-1)/IL-6 signaling pathways.
CI  - (c) The author(s).
FAU - Vo, Thi Thuy Tien
AU  - Vo TTT
AD  - School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, 
      Taiwan.
FAU - Lee, Chiang-Wen
AU  - Lee CW
AD  - Department of Orthopaedic Surgery, Chang Gung Memorial Hospital, Puzi City, 
      Chiayi County 61363, Taiwan.
AD  - Department of Nursing, Division of Basic Medical Sciences, and Chronic Diseases 
      and Health Promotion Research Center, and Research Center for Chinese Herbal 
      Medicine, Chang Gung University of Science and Technology, Puzi City, Chiayi 
      County 61363, Taiwan.
AD  - Department of Safety Health and Environmental Engineering, Ming Chi University of 
      Technology, New Taipei City 24301, Taiwan.
AD  - College of Medicine, Chang Gung University, Guishan Dist., Taoyuan City 33303, 
      Taiwan.
FAU - Wu, Ching-Zong
AU  - Wu CZ
AD  - School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, 
      Taiwan.
FAU - Liu, Ju-Fang
AU  - Liu JF
AD  - School of Oral Hygiene, College of Oral Medicine, Taipei Medical University, 
      Taipei, Taiwan.
FAU - Lin, Wei-Ning
AU  - Lin WN
AD  - Graduate Institute of Biomedical and Pharmaceutical Science, Fu Jen Catholic 
      University, New Taipei City, Taiwan.
FAU - Chen, Yuh-Lien
AU  - Chen YL
AD  - Department of Anatomy and Cell Biology, College of Medicine, National Taiwan 
      University, Taipei, Taiwan.
FAU - Hsu, Lee-Fen
AU  - Hsu LF
AD  - Department of Respiratory Care, Chang Gung University of Science and Technology, 
      Puzi City, Chiayi County 613, Taiwan.
AD  - Division of Neurosurgery, Department of Surgery, Chang Gung Memorial Hospital, 
      Puzi City, Chiayi County 613, Taiwan.
FAU - Tsai, Ming-Horng
AU  - Tsai MH
AD  - Department of Pediatrics, Division of Pediatric Hematology/Oncology and 
      Neonatology, Yunlin Chang Gung Memorial Hospital, Yunlin, Taiwan.
AD  - College of Medicine, Chang Gung University, Taoyuan, Taiwan.
FAU - Lee, I-Ta
AU  - Lee IT
AD  - School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, 
      Taiwan.
LA  - eng
PT  - Journal Article
DEP - 20200818
PL  - Australia
TA  - J Cancer
JT  - Journal of Cancer
JID - 101535920
PMC - PMC7477423
OTO - NOTNLM
OT  - Invasion
OT  - Matrix metalloproteinase
OT  - Oral cancer
OT  - Particulate matter
OT  - Surfactin
COIS- Competing Interests: The authors have declared that no competing interest exists.
EDAT- 2020/09/15 06:00
MHDA- 2020/09/15 06:01
PMCR- 2020/01/01
CRDT- 2020/09/14 05:51
PHST- 2020/05/17 00:00 [received]
PHST- 2020/08/06 00:00 [accepted]
PHST- 2020/09/14 05:51 [entrez]
PHST- 2020/09/15 06:00 [pubmed]
PHST- 2020/09/15 06:01 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - jcav11p6038 [pii]
AID - 10.7150/jca.48296 [doi]
PST - epublish
SO  - J Cancer. 2020 Aug 18;11(20):6038-6049. doi: 10.7150/jca.48296. eCollection 2020.