PMID- 32922601
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200916
IS  - 1936-2625 (Electronic)
IS  - 1936-2625 (Linking)
VI  - 13
IP  - 8
DP  - 2020
TI  - Correlations of SDF-1 and CXCR4 levels with caspase-3 expression in the retina of 
      rats after optic nerve injury.
PG  - 2058-2064
AB  - OBJECTIVE: This study explores the correlations of stromal cell-derived factor-1 
      (SDF-1) and CXC chemokine receptor 4 (CXCR4) levels with caspase-3 expression in 
      the retina of rats after optic nerve injury. MATERIALS AND METHODS: A total of 24 
      adult healthy specific pathogen-free Sprague-Dawley rats were selected and 
      randomly divided into an optic nerve injury group (n=16) and an optic nerve 
      sham-injury group (n=8). The optic nerve injury group was further sub-divided 
      into a 3 d group (n=8) and a 7 d group (n=8) after their injuries. In the optic 
      nerve injury group, the left eye of each rat was removed and prepared for the 
      optic nerve injury model using the optic nerve clamping method. In the 
      sham-injury group, the optic nerve in the left eye was only exposed without being 
      clamped. The rats were sacrificed at 3 d and 7 d after their optic nerve 
      injuries, and the retina was isolated. The expressions of SDF-1, CXCR4, and 
      caspase-3 in the retina of the rats in each group were measured using an 
      immunohistochemical method. Messenger ribonucleic acid (mRNA) and the protein 
      expressions of SDF-1, CXCR4, and caspase-3 (cleaved caspase-3) in the retinas of 
      rats were measured using quantitative real-time polymerase chain reaction (qPCR) 
      and western blotting, respectively. Moreover, the correlations of the expression 
      of SDF-1 and CXCR4 with caspase-3 expression were analyzed using the Spearman 
      method. The apoptosis of retinal ganglion cells of rats in each group was 
      observed using terminal deoxynucleotidyl transferase-mediated dUTP nick end 
      labeling (TUNEL) staining. RESULTS: Immunohistochemistry of the retinas revealed 
      that, compared with those in the sham-injury group, the expressions of SDF-1 and 
      CXCR4 in the retina in the 3 d group and the 7 d group were gradually increased. 
      Caspase-3 expression was significantly elevated at 3 d after the injuries, but 
      obviously decreased at 7 d after the injuries. The results of qPCR showed that 
      the relative expression levels of SDF-1 and CXCR4 mRNA in the retina at 3 d and 7 
      d after optic nerve injuries were also significantly higher than those in the 
      sham-injury group (P<0.01), and the caspase-3 mRNA expression was initially 
      increased at 3 d but reduced at 7 d after the injuries (P<0.01). Western blotting 
      for the detection of the SDF-1, CXCR4 and caspase-3 proteins indicated changes 
      similar to those of the qPCR. Spearman analysis results demonstrated that there 
      was a positive correlation between the SDF-1 and CXCR4 expressions, but the 
      expressions of SDF-1 and CXCR4 had negative correlations with caspase-3 
      expression. TUNEL staining showed that apoptosis of the retinal cells was 
      increased in the 3 d group but significantly decreased in the 7 d group. 
      CONCLUSION: After optic nerve injury, the continuous increase of the SDF-1 and 
      CXCR4 levels suppresses the apoptosis of retinal cells and repairs the retina by 
      inhibiting the cleavage activation of caspase-3. This provides new insights for 
      the ongoing treatment of optic nerve injury.
CI  - IJCEP Copyright (c) 2020.
FAU - Zhang, Xi
AU  - Zhang X
AD  - Departmental Health Check Section, Ningbo Eye Hospital Ningbo, Zhejiang, China.
FAU - Liao, Yanhong
AU  - Liao Y
AD  - Departmental Health Check Section, Ningbo Eye Hospital Ningbo, Zhejiang, China.
FAU - Ye, Ting
AU  - Ye T
AD  - Departmental Health Check Section, Ningbo Eye Hospital Ningbo, Zhejiang, China.
LA  - eng
PT  - Journal Article
DEP - 20200801
PL  - United States
TA  - Int J Clin Exp Pathol
JT  - International journal of clinical and experimental pathology
JID - 101480565
PMC - PMC7476942
OTO - NOTNLM
OT  - CXCR4
OT  - Optic nerve injury
OT  - SDF-1
OT  - apoptosis
OT  - caspase-3
COIS- None.
EDAT- 2020/09/15 06:00
MHDA- 2020/09/15 06:01
PMCR- 2020/08/01
CRDT- 2020/09/14 05:51
PHST- 2020/02/23 00:00 [received]
PHST- 2020/03/27 00:00 [accepted]
PHST- 2020/09/14 05:51 [entrez]
PHST- 2020/09/15 06:00 [pubmed]
PHST- 2020/09/15 06:01 [medline]
PHST- 2020/08/01 00:00 [pmc-release]
PST - epublish
SO  - Int J Clin Exp Pathol. 2020 Aug 1;13(8):2058-2064. eCollection 2020.