PMID- 32925988
OWN - NLM
STAT- MEDLINE
DCOM- 20210707
LR  - 20210707
IS  - 1734-154X (Electronic)
IS  - 0001-527X (Linking)
VI  - 67
IP  - 3
DP  - 2020 Sep 14
TI  - The Caprine casein-alpha-S2 protein modulates the molecular mechanism of insulin 
      signal transduction in type 2 diabetes rat.
PG  - 401-408
LID - 10.18388/abp.2020_5357 [doi]
AB  - This study purpose was to investigate the association of casein-alpha-S2 protein 
      of Caprine milk and molecular mechanismofinsulin signaltransduction in type2 
      diabetes mellitus (T2DM). The Caprine milk CSN1S2 protein treatment of 0, 375, 
      750, and 1500mg/kg BW were conducted to the control and T2DM rats. We observed 
      several physiological parameters of all rats. The levels of insulin and TNF-alpha in 
      the plasma were measured using ELISA.The expressions of proteins and mRNA levels 
      of diabetes-related genes in the pancreas tissues were analyzed using Western 
      Blotting and Real-Time PCR, respectively. Our study found that diabetic rats had 
      lower body weight, food intake, and fecal weight compared with control rats. The 
      Caprine milk CSN1S2 protein consumption affected the body weight of diabetic rats 
      to increase, especially at the dose of 750mg/kg BW.Interestingly, the genes 
      associated with insulin signaling were improved by the CSN1S2 protein treatment 
      in diabetic rats, although their blood glucose and cholesterol level were not 
      affected. The diabetic rats showed an elevated insulin level and GLUT4 protein 
      expression after treatment. We also reported that the CSN1S2-treated diabetic 
      rats had a gradually reduced expression of TNF-alpha and VCAM-1 in dose-dependent. 
      Moreover, the 750mg/kg BW of CSN1S2 treatment enhanced the mRNA expressions of 
      INS-receptor, GLUT4, IGF-1, CAMKK, and CAMKIV in diabetic rats. The ability of 
      Caprine milk CSN1S2 protein to regulate the molecular mechanisms in the 
      diabetes-signaling pathway indicated its potential therapeutic effects on 
      diabetes management.
FAU - Fatchiyah, Fatchiyah
AU  - Fatchiyah F
AD  - 1) Research Center of Smart Molecule of Natural Genetics Resources, Brawijaya 
      University 2) Biology Department, Faculty of Science, Brawijaya University.
FAU - Rohmah, Rista Nikmatu
AU  - Rohmah RN
AD  - 1Research Center of Smart Molecule of Natural Genetics Resource, Brawijaya 
      University, Malang, East Java, Indonesia, 2) Biosains Institute, Brawijaya 
      University, Malang, East Java, Indonesia.
FAU - Triprisila, Lidwina Faraline
AU  - Triprisila LF
AD  - 1)Research Center of Smart Molecule of Natural Genetics Resource, Brawijaya 
      University, Malang, East Java, Indonesia, 2) Biosains Institute, Brawijaya 
      University, Malang, East Java, Indonesia.
FAU - Ohta, Takeshi
AU  - Ohta T
AD  - 1)Research Center of Smart Molecule of Natural Genetics Resource, Brawijaya 
      University, Malang, East Java, Indonesia, 2) Graduate School of Agriculture, 
      Kyoto University, Kyoto, Japan.
FAU - Meidinna, Hazna Noor
AU  - Meidinna HN
AD  - 1)Research Center of Smart Molecule of Natural Genetics Resource, Brawijaya 
      University, Malang, East Java, Indonesia, 2)DBT-AIST International Laboratory for 
      Advanced Biomedicine (DAILAB), National Institute of Advanced Industrial Science 
      and Technology (AIST), and Tsukuba Life Science Innovation, Graduate School of 
      Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Acta Biochim Pol
JT  - Acta biochimica Polonica
JID - 14520300R
RN  - 0 (Blood Glucose)
RN  - 0 (Caseins)
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (Insulin)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Blood Glucose/analysis/drug effects
MH  - Body Weight/drug effects
MH  - Caseins/*administration & dosage
MH  - Cholesterol/blood
MH  - Diabetes Mellitus, Experimental/*blood/*drug therapy
MH  - Diabetes Mellitus, Type 2/*blood/*drug therapy
MH  - Goats
MH  - Hypoglycemic Agents/*administration & dosage
MH  - Insulin/*blood
MH  - Male
MH  - Milk/chemistry
MH  - Rats
MH  - Rats, Wistar
MH  - Signal Transduction/*drug effects
MH  - Treatment Outcome
MH  - Tumor Necrosis Factor-alpha/blood
EDAT- 2020/09/15 06:00
MHDA- 2021/07/08 06:00
CRDT- 2020/09/14 15:46
PHST- 2020/04/27 00:00 [received]
PHST- 2020/06/16 00:00 [accepted]
PHST- 2020/09/15 06:00 [pubmed]
PHST- 2021/07/08 06:00 [medline]
PHST- 2020/09/14 15:46 [entrez]
AID - 5357 [pii]
AID - 10.18388/abp.2020_5357 [doi]
PST - ppublish
SO  - Acta Biochim Pol. 2020 Sep 14;67(3):401-408. doi: 10.18388/abp.2020_5357.