PMID- 32928349
OWN - NLM
STAT- MEDLINE
DCOM- 20200916
LR  - 20200916
IS  - 1000-8020 (Print)
IS  - 1000-8020 (Linking)
VI  - 49
IP  - 4
DP  - 2020 Jul
TI  - [Determination of vitamin A and vitamin E in human serum by high performance 
      liquid chromatography with fluorescence detection].
PG  - 585-590
LID - 10.19813/j.cnki.weishengyanjiu.2020.04.011 [doi]
AB  - OBJECTIVE: To establish a method for the simultaneous and rapid determination of 
      vitamin A and vitamin E of different configurations in human serum by high 
      performance liquid chromatography(HPLC) with multi-wavelength fluorescence 
      detector. METHODS: The serum was mixed after adding internal standard, and 
      acetonitrile was added for protein precipitation. The mixture was centrifuged 
      after extraction with n-hexane. The n-hexane layer was dried by N_2 flow, the 
      residue was dissolved with methanol. The HPLC system was consisted of WATERS 
      Symmetry C_(18) column(4. 6 mm x 250 mm, 5 mum) and the mobile phase was methanol. 
      The column temperature was 30 ℃ and fluorescence detector with online wavelength 
      conversion method was carried out for the quantitative detection. RESULTS: The 
      liner range of determination of vitamin A, alpha-vitamin E, beta-vitamin E and delta-vitamin 
      E were 0. 050-2. 0 mug/mL, 0. 50-50 mug/mL, 0. 050-5. 0 mug/mL and 0. 050-5. 0 
      mug/mL, respectively(r>/=0. 996). The minimum detection limits of the method for 
      vitamin A and vitamin E were all 0. 02 mug/mL. The intraday and interday relative 
      standard deviations(RSDs) were less than 3% at high, medium and low 
      concentrations. The recoveries of the samples at the three concentrations were 
      91. 2%-107. 5%, and the RSDs were less than 10%. CONCLUSION: This method is 
      simple and accurate, with higher sensitivity than using UV detector and can be 
      used for the simultaneous determination of vitamin A and vitamin E of different 
      configurations in serum, and is suitable for rapid detection of batch serum 
      samples.
FAU - Zhao, Ying
AU  - Zhao Y
AD  - Center for Disease Control and Prevention of Shaanxi Province, Xi'an 710054, 
      China.
FAU - Hu, Jiawei
AU  - Hu J
AD  - Center for Disease Control and Prevention of Shaanxi Province, Xi'an 710054, 
      China.
FAU - Zhao, Jingjun
AU  - Zhao J
AD  - Center for Disease Control and Prevention of Shaanxi Province, Xi'an 710054, 
      China.
FAU - Yin, Danyang
AU  - Yin D
AD  - Center for Disease Control and Prevention of Shaanxi Province, Xi'an 710054, 
      China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Wei Sheng Yan Jiu
JT  - Wei sheng yan jiu = Journal of hygiene research
JID - 9426367
RN  - 11103-57-4 (Vitamin A)
RN  - 1406-18-4 (Vitamin E)
RN  - Y4S76JWI15 (Methanol)
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - Humans
MH  - Methanol
MH  - *Vitamin A
MH  - *Vitamin E
OTO - NOTNLM
OT  - fluorescence detector
OT  - high performance liquid chromatography
OT  - serum
OT  - vitamin A
OT  - vitamin E
EDAT- 2020/09/16 06:00
MHDA- 2020/09/17 06:00
CRDT- 2020/09/15 05:35
PHST- 2020/09/15 05:35 [entrez]
PHST- 2020/09/16 06:00 [pubmed]
PHST- 2020/09/17 06:00 [medline]
AID - 10.19813/j.cnki.weishengyanjiu.2020.04.011 [doi]
PST - ppublish
SO  - Wei Sheng Yan Jiu. 2020 Jul;49(4):585-590. doi: 
      10.19813/j.cnki.weishengyanjiu.2020.04.011.