PMID- 32929172
OWN - NLM
STAT- MEDLINE
DCOM- 20201208
LR  - 20211211
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 10
IP  - 1
DP  - 2020 Sep 14
TI  - Flexible ZnO-mAb nanoplatforms for selective peripheral blood mononuclear cell 
      immobilization.
PG  - 15018
LID - 10.1038/s41598-020-72133-0 [doi]
LID - 15018
AB  - Cancer is the second cause of death worldwide. This devastating disease requires 
      specific, fast, and affordable solutions to mitigate and reverse this trend. A 
      step towards cancer-fighting lies in the isolation of natural killer (NK) cells, 
      a set of innate immune cells, that can either be used as biomarkers of 
      tumorigenesis or, after autologous transplantation, to fight aggressive 
      metastatic cells. In order to specifically isolate NK cells (which express the 
      surface NKp30 receptor) from peripheral blood mononuclear cells, a ZnO 
      immunoaffinity-based platform was developed by electrodeposition of the metal 
      oxide on a flexible indium tin oxide (ITO)-coated polyethylene terephthalate 
      (PET) substrate. The resulting crystalline and well-aligned ZnO nanorods (NRs) 
      proved their efficiency in immobilizing monoclonal anti-human NKp30 antibodies 
      (mAb), obviating the need for additional procedures for mAb immobilization. The 
      presence of NK cells on the peripheral blood mononuclear cell (PBMCs) fraction 
      was evaluated by the response to their natural ligand (B7-H6) using an acridine 
      orange (AO)-based assay. The successful selection of NK cells from PBMCs by our 
      nanoplatform was assessed by the photoluminescent properties of AO. This easy and 
      straightforward ZnO-mAb nanoplatform paves the way for the design of biosensors 
      for clinic diagnosis, and, due to its inherent biocompatibility, for the initial 
      selection of NK cells for autotransplantation immunotherapies.
FAU - Babu, K Sowri
AU  - Babu KS
AD  - Division of Physics, Dept. Of Science and Humanities, Vignan's Foundation for 
      Science, Technology & Research (Deemed To Be University), Vadlamudi, Guntur, AP, 
      522213, India.
FAU - Pinheiro, Pedro F
AU  - Pinheiro PF
AD  - Centro de Quimica Estrutural, Instituto Superior Tecnico, Universidade de Lisboa, 
      Av. Rovisco Pais, 1049-001, Lisboa, Portugal.
FAU - Marques, Catia F
AU  - Marques CF
AD  - Centro de Quimica Estrutural, Instituto Superior Tecnico, Universidade de Lisboa, 
      Av. Rovisco Pais, 1049-001, Lisboa, Portugal.
FAU - Justino, Goncalo C
AU  - Justino GC
AD  - Centro de Quimica Estrutural, Instituto Superior Tecnico, Universidade de Lisboa, 
      Av. Rovisco Pais, 1049-001, Lisboa, Portugal. goncalo.justino@tecnico.ulisboa.pt.
FAU - Andrade, Suzana M
AU  - Andrade SM
AD  - Centro de Quimica Estrutural, Instituto Superior Tecnico, Universidade de Lisboa, 
      Av. Rovisco Pais, 1049-001, Lisboa, Portugal. suzana.andrade@tecnico.ulisboa.pt.
FAU - Alves, Marta M
AU  - Alves MM
AD  - Centro de Quimica Estrutural, Instituto Superior Tecnico, Universidade de Lisboa, 
      Av. Rovisco Pais, 1049-001, Lisboa, Portugal. martamalves@tecnico.ulisboa.pt.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200914
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Nanoconjugates)
RN  - 0 (Natural Cytotoxicity Triggering Receptor 3)
RN  - 0 (Polyethylene Terephthalates)
RN  - 0 (Tin Compounds)
RN  - 71243-84-0 (indium tin oxide)
RN  - SOI2LOH54Z (Zinc Oxide)
SB  - IM
EIN - Sci Rep. 2021 Dec 7;11(1):23900. PMID: 34876642
MH  - Antibodies, Monoclonal/*immunology
MH  - Biosensing Techniques/methods
MH  - Cell Separation/*methods
MH  - Cells, Immobilized/*cytology/immunology
MH  - Humans
MH  - Killer Cells, Natural/*cytology/immunology
MH  - Nanoconjugates/*chemistry
MH  - Natural Cytotoxicity Triggering Receptor 3/immunology
MH  - Polyethylene Terephthalates/chemistry
MH  - Tin Compounds/chemistry
MH  - Zinc Oxide/*chemistry
PMC - PMC7490409
COIS- The authors declare no competing interests.
EDAT- 2020/09/16 06:00
MHDA- 2020/12/15 06:00
PMCR- 2020/09/14
CRDT- 2020/09/15 05:45
PHST- 2020/01/29 00:00 [received]
PHST- 2020/08/25 00:00 [accepted]
PHST- 2020/09/15 05:45 [entrez]
PHST- 2020/09/16 06:00 [pubmed]
PHST- 2020/12/15 06:00 [medline]
PHST- 2020/09/14 00:00 [pmc-release]
AID - 10.1038/s41598-020-72133-0 [pii]
AID - 72133 [pii]
AID - 10.1038/s41598-020-72133-0 [doi]
PST - epublish
SO  - Sci Rep. 2020 Sep 14;10(1):15018. doi: 10.1038/s41598-020-72133-0.