PMID- 32929652
OWN - NLM
STAT- MEDLINE
DCOM- 20210518
LR  - 20210518
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 47
IP  - 10
DP  - 2020 Oct
TI  - ATP-binding cassette g1 regulates osteogenesis via Wnt/beta-catenin and AMPK 
      signaling pathways.
PG  - 7439-7449
LID - 10.1007/s11033-020-05800-0 [doi]
AB  - The dysfunction of bone marrow mesenchymal stem cells (BMSCs) in balancing 
      osteogenesis and adipogenesis plays an important role in the occurrence and 
      development of osteoporosis. It's still unknown that whether ATP-binding cassette 
      g1 (Abcg1), a well-proved regulation gene of adipogenesis, regulates 
      osteogenesis. In our previous study, we identified 30 differentially expressed 
      genes in osteogenesis and found the expression level ofAbcg1 negatively related 
      to osteogenesis among these genes. Abcg1 is a well-proven adipogenesis regulator 
      and cholesterol transporter, but it's role in osteogenesis remained unknown. In 
      this study we found it may control osteogenesis, further elucidating the exact 
      role of Abcg1 in regulating osteoblast differentiation would help propose new 
      strategies to prevent and treat osteoporosis. Therefore, we established Abcg1 up- 
      or down-expressed C3H10T1/2 and C2C12 cell lines and verified that Abcg1 
      knockdown enhanced expression of osteogenic factors runt-related transcription 
      factor 2 (Runx2) and alkaline phosphatase (ALP), while Abcg1 overexpression 
      reversed the promoting effect. Furthermore, we confirmed that Abcg1 modulated 
      osteogenesis via the Wnt/beta-catenin and AMPK signaling pathways. taken together, 
      these results suggest that Abcg1 may have an important role in regulating 
      osteogenesis via Wnt/beta-catenin and AMPK signaling pathways, and expect to be a 
      potential therapeutic target for osteoporosis.
FAU - Zhou, Lei
AU  - Zhou L
AD  - Department of Orthopedics, Shanghai Fifth People's Hospital, Fudan University, 
      Shanghai, China.
AD  - Department of Orthopaedic Surgery, Zhongshan Hospital, Fudan University, 
      Shanghai, China.
FAU - Sun, Shiwei
AU  - Sun S
AD  - Department of Orthopedics, Shanghai Fifth People's Hospital, Fudan University, 
      Shanghai, China.
FAU - Zhang, Tieqi
AU  - Zhang T
AD  - Department of Orthopedics, Shanghai Fifth People's Hospital, Fudan University, 
      Shanghai, China.
FAU - Yu, Yueming
AU  - Yu Y
AD  - Department of Orthopedics, Shanghai Fifth People's Hospital, Fudan University, 
      Shanghai, China.
FAU - Xu, Liang
AU  - Xu L
AD  - Department of Orthopedics, Shanghai Fifth People's Hospital, Fudan University, 
      Shanghai, China.
FAU - Li, Haoran
AU  - Li H
AD  - Department of Orthopedics, Shanghai Fifth People's Hospital, Fudan University, 
      Shanghai, China.
FAU - Wang, Minghai
AU  - Wang M
AUID- ORCID: 0000-0001-7538-5230
AD  - Department of Orthopedics, Shanghai Fifth People's Hospital, Fudan University, 
      Shanghai, China. wangminghai@5thhospital.com.
FAU - Hong, Yang
AU  - Hong Y
AD  - Department of Orthopedics, Shanghai Fifth People's Hospital, Fudan University, 
      Shanghai, China. hongyang@5thhospital.com.
LA  - eng
GR  - 2017MWTZ02/the Key Department of Minhang District/
GR  - 2017WYZDZK02/the Key Department of the Fifth People's Hospital of Shanghai/
GR  - 2018WYZT01/the Fifth People's Hospital of Shanghai, Fudan University/
GR  - 201512/the Minhang District Leading Talent Development Funds/
PT  - Journal Article
DEP - 20200914
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (ABCG1 protein, mouse)
RN  - 0 (ATP Binding Cassette Transporter, Subfamily G, Member 1)
RN  - 0 (CTNNB1 protein, mouse)
RN  - 0 (beta Catenin)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
SB  - IM
MH  - AMP-Activated Protein Kinases/genetics/*metabolism
MH  - ATP Binding Cassette Transporter, Subfamily G, Member 1/genetics/*metabolism
MH  - Animals
MH  - Cell Line
MH  - Mice
MH  - *Osteogenesis
MH  - *Wnt Signaling Pathway
MH  - beta Catenin/genetics/*metabolism
OTO - NOTNLM
OT  - AMPK
OT  - Abcg1
OT  - Osteogenesis
OT  - Osteoporosis
OT  - Wnt /beta-catenin
EDAT- 2020/09/16 06:00
MHDA- 2021/05/19 06:00
CRDT- 2020/09/15 05:49
PHST- 2020/06/07 00:00 [received]
PHST- 2020/08/29 00:00 [accepted]
PHST- 2020/09/16 06:00 [pubmed]
PHST- 2021/05/19 06:00 [medline]
PHST- 2020/09/15 05:49 [entrez]
AID - 10.1007/s11033-020-05800-0 [pii]
AID - 10.1007/s11033-020-05800-0 [doi]
PST - ppublish
SO  - Mol Biol Rep. 2020 Oct;47(10):7439-7449. doi: 10.1007/s11033-020-05800-0. Epub 
      2020 Sep 14.