PMID- 32929912
OWN - NLM
STAT- MEDLINE
DCOM- 20200916
LR  - 20220531
IS  - 1002-1892 (Print)
IS  - 1002-1892 (Linking)
VI  - 34
IP  - 9
DP  - 2020 Sep 15
TI  - [Three-dimensional printed Ti6Al4V-4Cu alloy promotes osteogenic gene expression 
      through bone immune regulation].
PG  - 1170-1176
LID - 10.7507/1002-1892.201912139 [doi]
AB  - OBJECTIVE: To investigate the effects of three-dimensional (3D) printed 
      Ti6Al4V-4Cu alloy on inflammation and osteogenic gene expression in mouse bone 
      marrow mesenchymal stem cells (BMSCs) and mouse mononuclear macrophage line 
      RAW264.7. METHODS: Ti6Al4V and Ti6Al4V-4Cu alloys were prepared by selective 
      laser melting, and the extracts of the two materials were prepared according to 
      the biological evaluation standard of medical devices. The effects of two kinds 
      of extracts on the proliferation of mouse BMSCs and mouse RAW264.7 cells were 
      detected by cell counting kit 8 method. After co-cultured with mouse BMSCs for 3 
      days, the expression of osteogenesis- related genes [collagen type Ⅰ (Col-Ⅰ), 
      alkaline phosphatase (ALP), Runx family transcription factor 2 (Runx-2), 
      osteoprotegerin (OPG), and osteopontin (OPN)] were detected by real-time 
      fluorescence quantitative PCR. After co-cultured with mouse RAW264.7 cells for 1 
      day, the expressions of inflammation-related genes [interleukin 4 (IL-4) and 
      nitric oxide synthase 2 (iNOS)] were detected by real-time fluorescence 
      quantitative PCR, and the supernatants of the two groups were collected to detect 
      the secretion of vascular endothelial growth factor a (VEGF-a) and bone 
      morphogenetic protein 2 (BMP-2) by ELISA. The osteogenic conditioned medium were 
      prepared with the supernatants of the two groups and co-cultured with BMSCs for 3 
      days. The expressions of osteogenesis-related genes (Col-Ⅰ, ALP, Runx-2, OPG, and 
      OPN) were detected by real-time fluorescence quantitative PCR. RESULTS: Compared 
      with Ti6Al4V alloy extract, Ti6Al4V-4Cu alloy extract had no obvious effect on 
      the proliferation of BMSCs and RAW264.7 cells, but it could promote the 
      expression of OPG mRNA in BMSCs, reduce the expression of iNOS mRNA in RAW264.7 
      cells, and promote the expression of IL-4 mRNA. It could also promote the 
      secretions of VEGF-a and BMP-2 in RAW264.7 cells. Ti6Al4V-4Cu osteogenic 
      conditioned medium could promote the expressions of Col-Ⅰ, ALP, Runx-2, OPG, and 
      OPN mRNAs in BMSCs. The differences were all significant ( P<0.05). CONCLUSION: 
      3D printed Ti6Al4V-4Cu alloy can promote RAW264.7 cells to secret VEGF-a and 
      BMP-2 by releasing copper ions, thus promoting osteogenesis through bone immune 
      regulation, which lays a theoretical foundation for the application of metal 
      prosthesis.
FAU - Zhang, Chenke
AU  - Zhang C
AD  - Department of Orthopedics, the First Affiliated Hospital of Army Medical 
      University of Chinese PLA, Chongqing, 400038, P.R.China;Department of 
      Orthopedics/Sports Medicine Center, the First Affiliated Hospital of Army Medical 
      University of Chinese PLA, Chongqing, 400038, P.R.China.
FAU - Lu, Yanjin
AU  - Lu Y
AD  - Fujian Institute of Research on the Structure of Matter, Fuzhou Fujian, 350002, 
      P.R.China.
FAU - Guo, Yupeng
AU  - Guo Y
AD  - Department of Orthopedics/Sports Medicine Center, the First Affiliated Hospital 
      of Army Medical University of Chinese PLA, Chongqing, 400038, P.R.China.
FAU - Chen, Wan
AU  - Chen W
AD  - Department of Orthopedics/Sports Medicine Center, the First Affiliated Hospital 
      of Army Medical University of Chinese PLA, Chongqing, 400038, P.R.China.
FAU - Tang, Hong
AU  - Tang H
AD  - Department of Orthopedics/Sports Medicine Center, the First Affiliated Hospital 
      of Army Medical University of Chinese PLA, Chongqing, 400038, P.R.China.
FAU - Li, Huaisheng
AU  - Li H
AD  - Department of Orthopedics/Sports Medicine Center, the First Affiliated Hospital 
      of Army Medical University of Chinese PLA, Chongqing, 400038, P.R.China.
FAU - Tang, Kanglai
AU  - Tang K
AD  - Department of Orthopedics/Sports Medicine Center, the First Affiliated Hospital 
      of Army Medical University of Chinese PLA, Chongqing, 400038, P.R.China.
FAU - He, Qingyi
AU  - He Q
AD  - Department of Orthopedics, the First Affiliated Hospital of Army Medical 
      University of Chinese PLA, Chongqing, 400038, P.R.China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
JT  - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = 
      Chinese journal of reparative and reconstructive surgery
JID - 9425194
RN  - 0 (Alloys)
RN  - 0 (BMP2 protein, human)
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 0 (VEGFA protein, human)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 12743-70-3 (titanium alloy (TiAl6V4))
RN  - D1JT611TNE (Titanium)
SB  - IM
MH  - *Alloys
MH  - Animals
MH  - Bone Marrow Cells
MH  - Bone Morphogenetic Protein 2/metabolism
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Gene Expression Regulation
MH  - Mice
MH  - *Osteogenesis
MH  - Titanium
MH  - Vascular Endothelial Growth Factor A/metabolism
PMC - PMC8171737
OTO - NOTNLM
OT  - Three-dimensional printing
OT  - Ti6Al4V-4Cu alloy
OT  - bone immune regulation
OT  - osteogenic microenvironment
COIS- 利益冲突：所有作者声明，在课题研究和文章撰写过程中不存在利益冲突。课题经费支持没有影响文章观点和对研究数据客观结果的统计分析及其报道。
EDAT- 2020/09/16 06:00
MHDA- 2020/09/17 06:00
PMCR- 2020/09/01
CRDT- 2020/09/15 05:53
PHST- 2020/09/15 05:53 [entrez]
PHST- 2020/09/16 06:00 [pubmed]
PHST- 2020/09/17 06:00 [medline]
PHST- 2020/09/01 00:00 [pmc-release]
AID - zgxfcjwkzz-34-9-1170 [pii]
AID - 10.7507/1002-1892.201912139 [doi]
PST - ppublish
SO  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2020 Sep 15;34(9):1170-1176. doi: 
      10.7507/1002-1892.201912139.