PMID- 32930186
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20210405
LR  - 20210405
IS  - 1759-9679 (Electronic)
IS  - 1759-9660 (Linking)
VI  - 12
IP  - 25
DP  - 2020 Jul 2
TI  - Polypyrrole-coated needle as an electrospray emitter for ambient mass 
      spectrometry.
PG  - 3235-3241
LID - 10.1039/d0ay00652a [doi]
AB  - Polypyrrole (PPy) is a polymer widely used as an extraction phase due to its 
      ability to perform intermolecular interactions with the analyte, such as 
      acid-base, pi-pi, dipole-dipole, hydrophobic, and hydrogen bonding. In this 
      manuscript, we report the coating of a stainless steel needle with a PPy film for 
      analyte extraction and subsequent analysis by electrospray ionization mass 
      spectrometry (ESI-MS) under ambient and open-air conditions. The method, named 
      PPy-ESI-MS, was optimized for analysis of 3,4-methylenedioxyamphetamine (MDA) and 
      3,4-methylenedioxymethamphetamine (MDMA) in synthetic urine. Seven cycles of 
      electrodeposition of the PPy film onto the needle surface, sample at pH 8, and 40 
      min of extraction of analytes were determined as the best analysis conditions. 
      The analytical performance of PPy-ESI-MS was evaluated for MDA and MDMA 
      compounds. Analytical curves were obtained with R2 > 0.98. Limits of detection 
      (LODs) and limits of quantification (LOQs) were determined as 20 mug L-1 and 70 mug 
      L-1 for MDA and as 25 mug L-1 and 80 mug L-1 for MDMA, respectively. Values of 
      precision were below 17%, and values of accuracy below 5%. The apparent 
      recoveries ranged between 84.5% and 111.3%. In addition, the PPy-ESI-MS method 
      was applied for the analysis of sarcosine in synthetic urine in order to evaluate 
      the performance of the method for another class of compounds. The calibration 
      curve was obtained with R2 > 0.98, along with LOD and LOQ of 30 mug L-1 and 100 mug 
      L-1, respectively. The precision and accuracy values were below 5% and 8%, 
      respectively, and the apparent recoveries close to 100%. This work demonstrates 
      the usefulness of combining an extraction phase with ESI-MS analysis under 
      ambient conditions to determine different classes of small molecules in a complex 
      sample.
FAU - de Araujo, Giovanna L
AU  - de Araujo GL
AD  - Chemistry Institute, Federal University of Goias, Goiania, Goias 74690-900, 
      Brazil. boniek@ufg.br.
FAU - de Aguiar, Deborah V A
AU  - de Aguiar DVA
AUID- ORCID: 0000-0002-8769-1339
AD  - Chemistry Institute, Federal University of Goias, Goiania, Goias 74690-900, 
      Brazil. boniek@ufg.br.
FAU - Pereira, Igor
AU  - Pereira I
AD  - Chemistry Institute, Federal University of Goias, Goiania, Goias 74690-900, 
      Brazil. boniek@ufg.br.
FAU - da Silva, Lidya C
AU  - da Silva LC
AD  - Chemistry Institute, Federal University of Goias, Goiania, Goias 74690-900, 
      Brazil. boniek@ufg.br.
FAU - Chaves, AndrA A R
AU  - Chaves AAR
AD  - Chemistry Institute, Federal University of Goias, Goiania, Goias 74690-900, 
      Brazil. boniek@ufg.br.
FAU - Vaz, Boniek G
AU  - Vaz BG
AUID- ORCID: 0000-0003-1197-4284
AD  - Chemistry Institute, Federal University of Goias, Goiania, Goias 74690-900, 
      Brazil. boniek@ufg.br.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Anal Methods
JT  - Analytical methods : advancing methods and applications
JID - 101519733
SB  - IM
EDAT- 2020/09/16 06:00
MHDA- 2020/09/16 06:01
CRDT- 2020/09/15 08:52
PHST- 2020/09/15 08:52 [entrez]
PHST- 2020/09/16 06:00 [pubmed]
PHST- 2020/09/16 06:01 [medline]
AID - 10.1039/d0ay00652a [doi]
PST - ppublish
SO  - Anal Methods. 2020 Jul 2;12(25):3235-3241. doi: 10.1039/d0ay00652a.