PMID- 32931602
OWN - NLM
STAT- MEDLINE
DCOM- 20211229
LR  - 20211229
IS  - 1470-8744 (Electronic)
IS  - 0885-4513 (Linking)
VI  - 68
IP  - 5
DP  - 2021 Oct
TI  - The effects of high-fat diet and metformin on urinary metabolites in diabetes and 
      prediabetes rat models.
PG  - 1014-1026
LID - 10.1002/bab.2021 [doi]
AB  - High-fat diet (HFD) interferes with the dietary plan of patients with type 2 
      diabetes mellitus (T2DM). However, many diabetes patients consume food with 
      higher fat content for a better taste bud experience. In this study, we examined 
      the effect of HFD on rats at the early onset of diabetes and prediabetes by 
      supplementing their feed with palm olein oil to provide a fat content 
      representing 39% of total calorie intake. Urinary profile generated from liquid 
      chromatography-mass spectrometry analysis was used to construct the orthogonal 
      partial least squares discriminant analysis (OPLS-DA) score plots. The data 
      provide insights into the physiological state of an organism. Healthy rats fed 
      with normal chow (NC) and HFD cannot be distinguished by their urinary metabolite 
      profiles, whereas diabetic and prediabetic rats showed a clear separation in 
      OPLS-DA profile between the two diets, indicating a change in their physiological 
      state. Metformin treatment altered the metabolomics profiles of diabetic rats and 
      lowered their blood sugar levels. For prediabetic rats, metformin treatment on 
      both NC- and HFD-fed rats not only reduced their blood sugar levels to normal but 
      also altered the urinary metabolite profile to be more like healthy rats. The use 
      of metformin is therefore beneficial at the prediabetes stage.
CI  - (c) 2020 International Union of Biochemistry and Molecular Biology, Inc.
FAU - Lee, Yan-Fen
AU  - Lee YF
AD  - USM-RIKEN International Centre of Aging Science, USM, Minden, Penang, Malaysia.
AD  - School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden, Penang, 
      Malaysia.
FAU - Sim, Xuan-Yi
AU  - Sim XY
AD  - USM-RIKEN International Centre of Aging Science, USM, Minden, Penang, Malaysia.
AD  - School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden, Penang, 
      Malaysia.
FAU - Teh, Ying-Hui
AU  - Teh YH
AD  - School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden, Penang, 
      Malaysia.
FAU - Ismail, Mohd Nazri
AU  - Ismail MN
AD  - Analytical Biochemistry Research Centre (ABrC), USM, Minden, Penang, Malaysia.
FAU - Greimel, Peter
AU  - Greimel P
AD  - Laboratory for Cell Function Dynamics, RIKEN Centre for Brain Sciences, Wako, 
      Saitama, Japan.
FAU - Murugaiyah, Vikneswaran
AU  - Murugaiyah V
AD  - School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden, Penang, 
      Malaysia.
FAU - Ibrahim, Baharudin
AU  - Ibrahim B
AD  - School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden, Penang, 
      Malaysia.
FAU - Gam, Lay-Harn
AU  - Gam LH
AUID- ORCID: 0000-0002-9274-4394
AD  - USM-RIKEN International Centre of Aging Science, USM, Minden, Penang, Malaysia.
AD  - School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden, Penang, 
      Malaysia.
LA  - eng
PT  - Journal Article
DEP - 20200928
PL  - United States
TA  - Biotechnol Appl Biochem
JT  - Biotechnology and applied biochemistry
JID - 8609465
RN  - 0 (Blood Glucose)
RN  - 0 (Hypoglycemic Agents)
RN  - 9100L32L2N (Metformin)
SB  - IM
MH  - Animals
MH  - Blood Glucose/metabolism
MH  - Diabetes Mellitus, Experimental/drug therapy/*metabolism/urine
MH  - Diabetes Mellitus, Type 2/drug therapy/*metabolism/urine
MH  - Diet, High-Fat/*adverse effects
MH  - Discriminant Analysis
MH  - *Disease Models, Animal
MH  - Hypoglycemic Agents/*metabolism/therapeutic use/urine
MH  - Least-Squares Analysis
MH  - Male
MH  - Metabolomics
MH  - Metformin/*metabolism/therapeutic use/urine
MH  - Rats
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - diet
OT  - liquid chromatography-mass spectrometry
OT  - metabolomics
OT  - metformin
OT  - type 2 diabetes mellitus
EDAT- 2020/09/16 06:00
MHDA- 2021/12/30 06:00
CRDT- 2020/09/15 17:14
PHST- 2020/03/30 00:00 [received]
PHST- 2020/08/31 00:00 [accepted]
PHST- 2020/09/16 06:00 [pubmed]
PHST- 2021/12/30 06:00 [medline]
PHST- 2020/09/15 17:14 [entrez]
AID - 10.1002/bab.2021 [doi]
PST - ppublish
SO  - Biotechnol Appl Biochem. 2021 Oct;68(5):1014-1026. doi: 10.1002/bab.2021. Epub 
      2020 Sep 28.